Detailed information NTG0627318

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: N1705_RS04640
• Genome accession: NZ_CP104123
• Coordinates: 940714..941694 (+)
• Length: 326 a.a.
• NCBI ID: WP_001055631.1
• Uniprot ID: -
• Organism: Escherichia coli strain USDA-ARS-USMARC-49610
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 935714..946694

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N1705_RS04610 (N1705_04610)	-	936087..937073 (-)	987	WP_000784004.1	TRAP transporter substrate-binding protein	-
N1705_RS04615 (N1705_04615)	hemW	937391..938527 (-)	1137	WP_000239917.1	radical SAM family heme chaperone HemW	-
N1705_RS04620 (N1705_04620)	rdgB	938520..939113 (-)	594	WP_001174735.1	XTP/dITP diphosphatase	-
N1705_RS04625 (N1705_04625)	yggU	939121..939411 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
N1705_RS04630 (N1705_04630)	yggT	939408..939974 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
N1705_RS04635 (N1705_04635)	yggS	939992..940696 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
N1705_RS04640 (N1705_04640)	pilT	940714..941694 (+)	981	WP_001055631.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
N1705_RS04645 (N1705_04645)	ruvX	941883..942299 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
N1705_RS04650 (N1705_04650)	yqgE	942299..942862 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
N1705_RS04655 (N1705_04655)	gshB	942971..943921 (-)	951	WP_000593273.1	glutathione synthase	-
N1705_RS04660 (N1705_04660)	rsmE	943934..944665 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
N1705_RS04665 (N1705_04665)	endA	944745..945452 (-)	708	WP_194875445.1	deoxyribonuclease I	-
N1705_RS04670 (N1705_04670)	yggI	945547..946044 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35946.11 Da
• Isoelectric Point: 5.7980

>NTDB_id=627318 N1705_RS04640 WP_001055631.1 940714..941694(+) (pilT) [Escherichia coli strain USDA-ARS-USMARC-49610]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=627318 N1705_RS04640 WP_001055631.1 940714..941694(+) (pilT) [Escherichia coli strain USDA-ARS-USMARC-49610]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	39.514	100	0.399
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	36.646	98.773	0.362
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362