Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LMH63_RS01720 Genome accession   NZ_CP086615
Coordinates   373868..374905 (+) Length   345 a.a.
NCBI ID   WP_109679457.1    Uniprot ID   A0A2U2MZ23
Organism   Spiribacter halobius strain E85     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 368868..379905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH63_RS01690 (LMH63_01690) - 369197..369628 (-) 432 WP_109679452.1 DUF4426 domain-containing protein -
  LMH63_RS01695 (LMH63_01695) metW 369702..370295 (-) 594 WP_109679477.1 methionine biosynthesis protein MetW -
  LMH63_RS01700 (LMH63_01700) - 370301..371455 (-) 1155 WP_109679453.1 homoserine O-acetyltransferase -
  LMH63_RS01705 (LMH63_01705) - 371653..372231 (-) 579 WP_109679454.1 YggT family protein -
  LMH63_RS01710 (LMH63_01710) proC 372234..373061 (-) 828 WP_109679455.1 pyrroline-5-carboxylate reductase -
  LMH63_RS01715 (LMH63_01715) - 373089..373778 (-) 690 WP_109679456.1 YggS family pyridoxal phosphate-dependent enzyme -
  LMH63_RS01720 (LMH63_01720) pilT 373868..374905 (+) 1038 WP_109679457.1 type IV pilus twitching motility protein PilT Machinery gene
  LMH63_RS01725 (LMH63_01725) pilU 374938..376056 (+) 1119 WP_109679458.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LMH63_RS01730 (LMH63_01730) - 376350..377042 (-) 693 WP_109679459.1 hypothetical protein -
  LMH63_RS01735 (LMH63_01735) - 377143..378432 (-) 1290 WP_109679460.1 dihydroorotase -
  LMH63_RS01740 (LMH63_01740) - 378429..379397 (-) 969 WP_109679461.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38300.81 Da        Isoelectric Point: 6.7299

>NTDB_id=625703 LMH63_RS01720 WP_109679457.1 373868..374905(+) (pilT) [Spiribacter halobius strain E85]
MDIADLLAFGVKNNASDLHISAGLPPMIRVDGDVRRINLPAMEHKEVHGLIYDIMNDKQRKDYEEYLETDFSFEIKGLAR
FRVNAFNHNRGAGAVFRTIPSTVLTLEELGAPDVFKTISDNPRGIVLVTGPTGSGKSTTLAAMIDYKNENYHEHILTIED
PIEFVHESKKSLINQREVHRDTLGFSEALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKSMVRSMLSESLRAVISQTLLKKVGGGRIAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGQSTGMQTLDQCL
QNLIRKGQITKQDARLKAVNREQFS

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=625703 LMH63_RS01720 WP_109679457.1 373868..374905(+) (pilT) [Spiribacter halobius strain E85]
ATGGATATCGCGGACCTGCTCGCCTTCGGCGTCAAGAACAACGCCTCCGATCTGCACATCTCCGCGGGGCTGCCGCCGAT
GATACGGGTGGACGGCGATGTGCGTCGAATCAACCTGCCGGCCATGGAGCACAAGGAGGTGCACGGGCTCATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAAGAGTACCTGGAGACGGACTTCTCGTTCGAGATCAAGGGACTTGCGCGC
TTCCGCGTCAATGCGTTCAACCACAACCGCGGCGCCGGCGCAGTATTCCGGACCATCCCGTCCACCGTGCTCACCCTCGA
GGAGCTGGGGGCACCCGATGTCTTCAAGACCATCTCCGACAACCCCCGCGGCATCGTGCTGGTCACCGGGCCCACCGGCT
CCGGCAAGTCCACCACGCTGGCGGCGATGATCGACTACAAGAACGAGAACTACCACGAGCACATCCTCACCATCGAGGAT
CCGATCGAGTTCGTGCATGAGTCGAAGAAGAGCCTGATCAATCAGCGCGAGGTCCACCGCGACACTCTCGGCTTCTCCGA
GGCGCTGCGCTCGGCCCTGCGCGAGGACCCGGACGTGGTGCTGGTCGGCGAGCTGCGCGATCTCGAGACCATCCGCCTGG
CGCTCACCGCAGCGGAGACCGGGCACCTGGTGTTCGGCACGCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTCTTCCCCGCCGCGGAGAAGTCCATGGTGCGCTCCATGCTCTCCGAGTCCCTGCGTGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGGTCGGCGGCGGGCGCATCGCCGCCCACGAGATCATGATCGGCACCCCCGCCATCCGGAACCTCATCC
GCGAGGACAAGGTGGCGCAGATGTACTCCGCCATCCAGACCGGTCAGTCCACCGGCATGCAGACCCTCGATCAGTGCCTG
CAGAACCTCATCCGCAAGGGCCAGATCACCAAGCAGGACGCCCGCCTGAAGGCGGTCAACCGCGAGCAGTTCAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U2MZ23

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.105

99.71

0.809
  pilT Pseudomonas stutzeri DSM 10701

81.105

99.71

0.809
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

79.07

99.71

0.788
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Neisseria meningitidis 8013

69.653

100

0.699
  pilT Neisseria gonorrhoeae MS11

69.364

100

0.696
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.681

0.684
  pilT Vibrio cholerae strain A1552

70.03

97.681

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.289

100

0.504
  pilU Pseudomonas stutzeri DSM 10701

43.149

99.42

0.429
  pilU Vibrio cholerae strain A1552

40.299

97.101

0.391
  pilU Acinetobacter baylyi ADP1

39.881

97.391

0.388