Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LMH63_RS01725 Genome accession   NZ_CP086615
Coordinates   374938..376056 (+) Length   372 a.a.
NCBI ID   WP_109679458.1    Uniprot ID   A0A2U2MYM2
Organism   Spiribacter halobius strain E85     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 369938..381056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH63_RS01700 (LMH63_01700) - 370301..371455 (-) 1155 WP_109679453.1 homoserine O-acetyltransferase -
  LMH63_RS01705 (LMH63_01705) - 371653..372231 (-) 579 WP_109679454.1 YggT family protein -
  LMH63_RS01710 (LMH63_01710) proC 372234..373061 (-) 828 WP_109679455.1 pyrroline-5-carboxylate reductase -
  LMH63_RS01715 (LMH63_01715) - 373089..373778 (-) 690 WP_109679456.1 YggS family pyridoxal phosphate-dependent enzyme -
  LMH63_RS01720 (LMH63_01720) pilT 373868..374905 (+) 1038 WP_109679457.1 type IV pilus twitching motility protein PilT Machinery gene
  LMH63_RS01725 (LMH63_01725) pilU 374938..376056 (+) 1119 WP_109679458.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LMH63_RS01730 (LMH63_01730) - 376350..377042 (-) 693 WP_109679459.1 hypothetical protein -
  LMH63_RS01735 (LMH63_01735) - 377143..378432 (-) 1290 WP_109679460.1 dihydroorotase -
  LMH63_RS01740 (LMH63_01740) - 378429..379397 (-) 969 WP_109679461.1 aspartate carbamoyltransferase catalytic subunit -
  LMH63_RS01745 (LMH63_01745) pyrR 379394..379906 (-) 513 WP_109679462.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LMH63_RS01750 (LMH63_01750) ruvX 379903..380319 (-) 417 WP_109679463.1 Holliday junction resolvase RuvX -
  LMH63_RS01755 (LMH63_01755) - 380326..380886 (-) 561 WP_109679464.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41346.44 Da        Isoelectric Point: 6.4334

>NTDB_id=625704 LMH63_RS01725 WP_109679458.1 374938..376056(+) (pilU) [Spiribacter halobius strain E85]
MDFNALLELLVNKKGSDLFITAGLPPSMKVAGRIMPITKNPLGAEQARQVVYSIMSERQREEFDATHECNFAISAKGLGR
FRVSAFYQRNNVGMVLRRIETEIPSIEALRLPPILKDLSMTKRGLIIFVGATGTGKSTSLASMIGYRNHNSTGHIITIED
PIEYMHAHQGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEIRARETMDYAIAFAETGHLCLATLHANNANQAMDRI
INFFPSDRRDQLFTDLSLNLKAVIAQQLIPTPDGKGRVPAVEILIGTPLVADKIHNGEVHELKEIMKRSGEQGMQTFDRH
LYQLYAEGSITYEDALRFADSANEVRLMAKLGDSHAEARMQQATEGIKLVDE

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=625704 LMH63_RS01725 WP_109679458.1 374938..376056(+) (pilU) [Spiribacter halobius strain E85]
ATGGATTTCAATGCCCTGCTCGAGCTACTGGTCAACAAGAAGGGCTCGGACCTGTTCATCACCGCGGGCCTGCCGCCGAG
CATGAAGGTGGCCGGGCGGATCATGCCCATCACCAAGAACCCCCTCGGCGCCGAGCAGGCCCGGCAGGTGGTCTACTCGA
TCATGAGCGAGCGCCAGCGCGAGGAGTTCGACGCCACCCACGAGTGCAACTTTGCCATCAGCGCGAAGGGCCTCGGGCGC
TTCCGCGTCAGCGCCTTCTACCAGCGCAACAACGTGGGCATGGTGCTGCGCCGGATCGAGACCGAGATCCCGAGCATCGA
GGCGCTGCGGCTGCCGCCGATCCTGAAGGATCTGTCCATGACCAAGCGCGGGCTGATCATCTTCGTCGGCGCCACGGGCA
CCGGCAAGTCCACCTCGCTTGCCTCGATGATCGGCTACCGCAACCACAACTCCACCGGACACATCATCACCATCGAGGAC
CCCATCGAGTACATGCACGCCCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGCATCGATACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCCTGATCGGCGAGATCCGCGCCCGCGAGACCATGGACTACG
CCATCGCCTTCGCCGAGACCGGTCACCTGTGCCTGGCGACGCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATC
ATCAACTTCTTCCCCTCGGACCGCCGCGATCAGCTCTTCACCGACCTCTCGCTGAACCTGAAGGCGGTGATTGCCCAGCA
GCTCATCCCGACCCCGGACGGCAAGGGCCGCGTGCCGGCGGTGGAGATTCTCATCGGCACCCCGCTGGTCGCGGACAAGA
TCCACAACGGCGAGGTGCACGAGCTGAAGGAGATCATGAAGCGTTCCGGCGAGCAGGGCATGCAGACCTTCGACCGCCAT
CTCTATCAGCTCTACGCCGAGGGCTCCATCACCTACGAGGACGCCCTGCGCTTCGCCGACTCCGCCAACGAGGTCCGCCT
GATGGCCAAGCTCGGCGACTCCCACGCCGAGGCGCGCATGCAGCAGGCGACGGAGGGGATCAAGCTCGTGGACGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U2MYM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

70.19

99.194

0.696
  pilU Acinetobacter baylyi ADP1

65.537

95.161

0.624
  pilU Vibrio cholerae strain A1552

54.821

97.581

0.535
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.553

93.817

0.409
  pilT Legionella pneumophila strain Lp02

41.791

90.054

0.376
  pilT Legionella pneumophila strain ERS1305867

41.791

90.054

0.376
  pilT Pseudomonas aeruginosa PAK

41.194

90.054

0.371
  pilT Acinetobacter baylyi ADP1

39.883

91.667

0.366