Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KT882_RS06715 Genome accession   NZ_CP086370
Coordinates   1532412..1533455 (-) Length   347 a.a.
NCBI ID   WP_161071632.1    Uniprot ID   A0A7X4HAH1
Organism   Pseudoduganella aquatica strain HC52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1527412..1538455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KT882_RS06705 - 1530779..1531264 (-) 486 WP_161071634.1 glutathione peroxidase -
  KT882_RS06710 pilU 1531273..1532409 (-) 1137 WP_161071633.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KT882_RS06715 pilT 1532412..1533455 (-) 1044 WP_161071632.1 type IV pilus twitching motility protein PilT Machinery gene
  KT882_RS06720 - 1533524..1534237 (+) 714 WP_228895044.1 YggS family pyridoxal phosphate-dependent enzyme -
  KT882_RS06725 proC 1534305..1535174 (+) 870 WP_228895046.1 pyrroline-5-carboxylate reductase -
  KT882_RS06730 - 1535273..1535620 (-) 348 WP_161071629.1 YqjD family protein -
  KT882_RS06735 ubiA 1535662..1536516 (-) 855 WP_161071628.1 4-hydroxybenzoate octaprenyltransferase -
  KT882_RS06740 - 1536571..1537515 (-) 945 WP_161071627.1 hydrogen peroxide-inducible genes activator -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38017.68 Da        Isoelectric Point: 6.7267

>NTDB_id=624556 KT882_RS06715 WP_161071632.1 1532412..1533455(-) (pilT) [Pseudoduganella aquatica strain HC52]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHGMIYDIMNDGQRKAYEEMLECDFSFAIPGLAR
FRVNAYNQERGAAAVLRTIPSKILTLEELNAPKIFAEFALKPRGLVLVTGPTGSGKSTTLAAMVNHLNENEYGHILTIED
PIEFVHDSKKCLINQREVGPHTLSFNNALRSALREDPDAILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMVGTPAIRNLIREAKIAQMYSAIQTGSNVGMQTLDQN
LTELVRRNVISSAAARNAAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=624556 KT882_RS06715 WP_161071632.1 1532412..1533455(-) (pilT) [Pseudoduganella aquatica strain HC52]
ATGGACATCTCCGAATTACTCGCATTCTCCGTCAAGAACAAAGCCTCCGATTTGCACCTGTCCGCCGGCCTGCCGCCCAT
GATCCGCGTGCACGGCGACGTGCGCCGCATCAATCTGCCGCCGCTCGAGCACAAGGACGTGCACGGCATGATCTATGACA
TCATGAATGACGGCCAGCGCAAGGCCTACGAGGAAATGCTGGAGTGCGACTTCTCCTTCGCCATTCCGGGCCTGGCGCGC
TTCCGCGTCAACGCCTACAACCAGGAGCGCGGCGCGGCCGCCGTGCTGCGCACCATTCCGTCCAAGATCCTCACGCTGGA
GGAGCTGAACGCGCCCAAGATCTTCGCCGAATTCGCGCTCAAGCCGCGCGGCCTGGTGCTGGTGACGGGGCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGGTCAACCACCTCAATGAAAACGAATACGGCCACATCCTCACCATCGAGGAC
CCGATCGAGTTCGTGCACGACTCCAAGAAATGCCTGATCAACCAGCGCGAAGTGGGGCCGCACACGCTGTCGTTCAACAA
CGCGCTGCGCTCGGCGCTGCGGGAAGACCCGGACGCCATCCTGGTCGGCGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGTCGGCGGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCCTCCGCCGCCAAGACCATCGACCGTATC
GTGGACGTATTCCCGGCCGAGGAAAAAGAGATGGTGCGGGCCATGCTGTCCGAATCGCTGCAGGCGGTCATCTCGCAGAC
CCTGCTCAAGACCAAGGACGGCTCCGGCCGCGTGGCCGCGCACGAGATCATGGTCGGCACGCCGGCCATCCGCAACCTGA
TACGCGAAGCCAAGATCGCGCAGATGTACTCGGCCATCCAGACCGGCAGCAATGTCGGCATGCAGACGCTGGACCAGAAC
CTGACCGAGCTGGTGCGCCGCAACGTGATCTCCTCGGCCGCCGCCCGCAACGCCGCCAAGATCCCCGACAACTTCCCGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X4HAH1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.101

99.424

0.767
  pilT Acinetobacter baumannii D1279779

77.101

99.424

0.767
  pilT Acinetobacter baumannii strain A118

77.101

99.424

0.767
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

75.516

97.695

0.738
  pilT Legionella pneumophila strain ERS1305867

75.516

97.695

0.738
  pilT Neisseria meningitidis 8013

69.706

97.983

0.683
  pilT Neisseria gonorrhoeae MS11

69.706

97.983

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.277

95.677

0.663
  pilT Vibrio cholerae strain A1552

69.277

95.677

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401
  pilU Vibrio cholerae strain A1552

41.003

97.695

0.401