Detailed information NTG0624555

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KT882_RS06710
• Genome accession: NZ_CP086370
• Coordinates: 1531273..1532409 (-)
• Length: 378 a.a.
• NCBI ID: WP_161071633.1
• Uniprot ID: A0A7X4KKM6
• Organism: Pseudoduganella aquatica strain HC52
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1526273..1537409

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KT882_RS06700	-	1527281..1530730 (-)	3450	WP_161071635.1	MHYT domain-containing protein	-
KT882_RS06705	-	1530779..1531264 (-)	486	WP_161071634.1	glutathione peroxidase	-
KT882_RS06710	pilU	1531273..1532409 (-)	1137	WP_161071633.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KT882_RS06715	pilT	1532412..1533455 (-)	1044	WP_161071632.1	type IV pilus twitching motility protein PilT	Machinery gene
KT882_RS06720	-	1533524..1534237 (+)	714	WP_228895044.1	YggS family pyridoxal phosphate-dependent enzyme	-
KT882_RS06725	proC	1534305..1535174 (+)	870	WP_228895046.1	pyrroline-5-carboxylate reductase	-
KT882_RS06730	-	1535273..1535620 (-)	348	WP_161071629.1	YqjD family protein	-
KT882_RS06735	ubiA	1535662..1536516 (-)	855	WP_161071628.1	4-hydroxybenzoate octaprenyltransferase	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 41848.20 Da
• Isoelectric Point: 6.4213

>NTDB_id=624555 KT882_RS06710 WP_161071633.1 1531273..1532409(-) (pilU) [Pseudoduganella aquatica strain HC52]
MERDQASKFMFDLLRLMVSKNGSDLFITAGFPPAMKIDGKMTPVSAQPLTAAHTSDLARSIMNDKQTAGFELTKEANFAI
SPGDLGRFRVSAFVQMSAVGMVLRTINSAIPKLEDLGLPEVLKDVVMSKRGLVIMVGATGSGKSTTLAAMVGYRNENSYG
HIITIEDPVEFVHPHRNCVITQREVGVDTDDWEVALKNTLRQAPDVIQIGEIRDRQTMDHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLVPRKETKGRAVAIEILLNSPLISDLIFKGQVHEIKELMKKSRELG
MQTFDQSLFDLYEADAISYEDALRNADSVNDLRLAIKLEGKAAKTRDLSAGTEHLNII

Nucleotide

• Length: 1137 bp

>NTDB_id=624555 KT882_RS06710 WP_161071633.1 1531273..1532409(-) (pilU) [Pseudoduganella aquatica strain HC52]
ATGGAACGCGACCAGGCATCCAAATTCATGTTCGACCTCTTGCGCCTGATGGTCAGCAAGAACGGTTCGGACTTGTTCAT
TACCGCCGGCTTCCCGCCGGCCATGAAGATAGACGGCAAGATGACGCCGGTGTCGGCCCAGCCGCTGACGGCGGCCCACA
CGTCGGATCTGGCCCGCTCCATCATGAACGACAAGCAGACCGCCGGCTTCGAGCTGACCAAGGAGGCCAACTTCGCCATC
AGCCCCGGCGACCTGGGCCGCTTCCGCGTGTCCGCCTTCGTGCAGATGAGCGCGGTCGGCATGGTGCTGCGCACGATTAA
TTCGGCCATCCCCAAGCTGGAGGACCTGGGCCTGCCCGAAGTGCTCAAGGACGTGGTGATGTCCAAGCGCGGCCTGGTCA
TCATGGTGGGCGCCACCGGCTCGGGCAAGTCCACCACGCTGGCGGCCATGGTGGGCTACCGCAACGAGAACAGCTACGGC
CACATCATCACCATCGAGGACCCGGTGGAGTTCGTGCACCCGCACCGCAACTGCGTGATCACCCAGCGCGAAGTGGGCGT
CGATACCGACGACTGGGAAGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAGATCCGCG
ACCGCCAGACCATGGACCACGCCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAGGAGCGCCGCCAGCAGCTGCTGATGGACTTGTCGCTCAACCT
GAAGGGCATGATTTCGCAGCGCCTGGTGCCGCGCAAGGAGACCAAGGGCCGCGCCGTGGCCATCGAGATCCTGCTCAATT
CGCCGCTGATCTCGGACCTGATCTTCAAGGGCCAGGTGCACGAGATCAAGGAGCTGATGAAAAAATCCCGCGAGCTGGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTACGAGGCCGACGCCATCAGCTATGAGGACGCGCTGCGCAACGCCGA
TTCCGTCAACGACCTGCGGCTGGCCATCAAGCTGGAAGGCAAGGCCGCCAAGACGCGCGACCTGTCGGCCGGCACCGAAC
ACCTGAACATTATTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (70-277)

3D structure

• AlphaFold DB: A0A7X4KKM6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	65.318	91.534	0.598
pilU	Acinetobacter baylyi ADP1	59.104	94.444	0.558
pilU	Vibrio cholerae strain A1552	56.891	90.212	0.513
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.582	88.624	0.386
pilT	Pseudomonas aeruginosa PAK	42.388	88.624	0.376
pilT	Acinetobacter baumannii D1279779	42.598	87.566	0.373
pilT	Acinetobacter nosocomialis M2	42.598	87.566	0.373
pilT	Acinetobacter baumannii strain A118	42.598	87.566	0.373
pilT	Acinetobacter baylyi ADP1	43.125	84.656	0.365
pilT	Pseudomonas stutzeri DSM 10701	40.896	88.624	0.362