Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KFS85_RS13955 Genome accession   NZ_CP086332
Coordinates   3393408..3394445 (-) Length   345 a.a.
NCBI ID   WP_003471666.1    Uniprot ID   A0A0K2ZRY0
Organism   Xanthomonas graminis pv. phlei strain LMG 843     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3388408..3399445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFS85_RS13935 (KFS85_13955) - 3388836..3389402 (-) 567 WP_009599956.1 YqgE/AlgH family protein -
  KFS85_RS13940 (KFS85_13960) - 3389510..3391318 (+) 1809 WP_237651995.1 DUF4153 domain-containing protein -
  KFS85_RS13945 (KFS85_13965) - 3391334..3391873 (+) 540 WP_237651996.1 DNA-3-methyladenine glycosylase I -
  KFS85_RS13950 (KFS85_13970) pilU 3392129..3393259 (-) 1131 WP_053839050.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KFS85_RS13955 (KFS85_13975) pilT 3393408..3394445 (-) 1038 WP_003471666.1 type IV pilus twitching motility protein PilT Machinery gene
  KFS85_RS13960 (KFS85_13980) - 3394570..3395220 (+) 651 WP_053839061.1 YggS family pyridoxal phosphate-dependent enzyme -
  KFS85_RS13965 (KFS85_13985) - 3395615..3396661 (+) 1047 WP_237651997.1 hypothetical protein -
  KFS85_RS13970 (KFS85_13990) proC 3396791..3397633 (+) 843 WP_053839052.1 pyrroline-5-carboxylate reductase -
  KFS85_RS13975 (KFS85_13995) - 3397648..3398103 (+) 456 WP_237651998.1 DUF4426 domain-containing protein -
  KFS85_RS13980 (KFS85_14000) - 3398269..3399081 (-) 813 WP_212590187.1 IS110 family transposase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38599.24 Da        Isoelectric Point: 6.6650

>NTDB_id=624345 KFS85_RS13955 WP_003471666.1 3393408..3394445(-) (pilT) [Xanthomonas graminis pv. phlei strain LMG 843]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMQTLDQHL
QDLVKRSLITRNQARDYAKDKRVFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=624345 KFS85_RS13955 WP_003471666.1 3393408..3394445(-) (pilT) [Xanthomonas graminis pv. phlei strain LMG 843]
ATGGATATCGCTGAACTATTGGCGTTCTCGGTCAAGAACAAGGCGTCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTGCGCCGCATCAATATCCCTGCACTGGACCACAAGCAGGTGCATGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAAGAGTTCCTCGAAGTGGACTTCTCGTTCGAGATTCCCAGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACCCTGGA
AGACCTCGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGATTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCAATCGAATTCGTGCACACCTCGCAGAAATGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
AGCGTTGCGCTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCGTGGGAGATCATGGTCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGTACGGCATGCAGACCCTGGACCAGCACCTG
CAGGATCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCGCGCGACTACGCCAAGGACAAGCGGGTCTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K2ZRY0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

78.779

99.71

0.786
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

40.23

100

0.406
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397