Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KFS85_RS13950 Genome accession   NZ_CP086332
Coordinates   3392129..3393259 (-) Length   376 a.a.
NCBI ID   WP_053839050.1    Uniprot ID   A0A0K3A206
Organism   Xanthomonas graminis pv. phlei strain LMG 843     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3387129..3398259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFS85_RS13925 (KFS85_13945) - 3387379..3388326 (-) 948 WP_053834932.1 aspartate carbamoyltransferase catalytic subunit -
  KFS85_RS13930 (KFS85_13950) ruvX 3388346..3388843 (-) 498 WP_053839048.1 Holliday junction resolvase RuvX -
  KFS85_RS13935 (KFS85_13955) - 3388836..3389402 (-) 567 WP_009599956.1 YqgE/AlgH family protein -
  KFS85_RS13940 (KFS85_13960) - 3389510..3391318 (+) 1809 WP_237651995.1 DUF4153 domain-containing protein -
  KFS85_RS13945 (KFS85_13965) - 3391334..3391873 (+) 540 WP_237651996.1 DNA-3-methyladenine glycosylase I -
  KFS85_RS13950 (KFS85_13970) pilU 3392129..3393259 (-) 1131 WP_053839050.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KFS85_RS13955 (KFS85_13975) pilT 3393408..3394445 (-) 1038 WP_003471666.1 type IV pilus twitching motility protein PilT Machinery gene
  KFS85_RS13960 (KFS85_13980) - 3394570..3395220 (+) 651 WP_053839061.1 YggS family pyridoxal phosphate-dependent enzyme -
  KFS85_RS13965 (KFS85_13985) - 3395615..3396661 (+) 1047 WP_237651997.1 hypothetical protein -
  KFS85_RS13970 (KFS85_13990) proC 3396791..3397633 (+) 843 WP_053839052.1 pyrroline-5-carboxylate reductase -
  KFS85_RS13975 (KFS85_13995) - 3397648..3398103 (+) 456 WP_237651998.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41974.30 Da        Isoelectric Point: 6.8811

>NTDB_id=624344 KFS85_RS13950 WP_053839050.1 3392129..3393259(-) (pilU) [Xanthomonas graminis pv. phlei strain LMG 843]
MSTIDFTSFLKLMAHQKASDLFITAGMPPAIKVHGKITPITQTPLTSQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNMRGVVAQQLIPTPDGKSRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=624344 KFS85_RS13950 WP_053839050.1 3392129..3393259(-) (pilU) [Xanthomonas graminis pv. phlei strain LMG 843]
ATGAGCACCATCGACTTCACCTCGTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCGCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCTCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATCCC
CACCGTCGATGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCGATCTCCTTCGCCGAGACCGGCCACCTGGTGTTGTGCACCCTGCATGCCAACAACGCCAATCAGGCGATG
GACCGGATCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACCTGTCGCTGAACATGCGCGGCGTGGT
CGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGAGCCGCCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAATCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCAGGACCCTGGCGCAGGGCATGGACGGGGTGGAAATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K3A206

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.583

98.138

0.644
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.286

93.085

0.394
  pilT Pseudomonas aeruginosa PAK

40.708

90.16

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367