Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LLY41_RS05795 Genome accession   NZ_CP086235
Coordinates   1165420..1166475 (+) Length   351 a.a.
NCBI ID   WP_304587143.1    Uniprot ID   -
Organism   Cytobacillus firmus strain T8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1160420..1171475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLY41_RS05780 (LLY41_05790) - 1161601..1162335 (+) 735 WP_304587141.1 PRC-barrel domain-containing protein -
  LLY41_RS05785 (LLY41_05795) - 1162355..1163728 (+) 1374 WP_304587142.1 VanW family protein -
  LLY41_RS05790 (LLY41_05800) - 1163750..1165410 (+) 1661 Protein_1101 GspE/PulE family protein -
  LLY41_RS05795 (LLY41_05805) pilT 1165420..1166475 (+) 1056 WP_304587143.1 type IV pilus twitching motility protein PilT Machinery gene
  LLY41_RS05800 (LLY41_05810) - 1166462..1167667 (+) 1206 WP_304587144.1 type II secretion system F family protein -
  LLY41_RS05805 (LLY41_05815) - 1167773..1168192 (+) 420 WP_304587145.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LLY41_RS05810 (LLY41_05820) comC 1168207..1168950 (+) 744 WP_304587146.1 A24 family peptidase Machinery gene
  LLY41_RS05815 (LLY41_05825) pilM 1169039..1170034 (+) 996 WP_304587147.1 type IV pilus biogenesis protein PilM -
  LLY41_RS05820 (LLY41_05830) - 1170038..1170697 (+) 660 WP_304587148.1 fimbrial assembly protein -
  LLY41_RS05825 (LLY41_05835) - 1170694..1171473 (+) 780 WP_304587149.1 pilus assembly protein PilO -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38842.03 Da        Isoelectric Point: 9.3870

>NTDB_id=623892 LLY41_RS05795 WP_304587143.1 1165420..1166475(+) (pilT) [Cytobacillus firmus strain T8]
MKTKIDYLLRAAYELKASDLHLTVGVPPVIRINGDLKKYGKDSLRPEDTEGMAKAIIPANIWKKFEENGELDFSYGIPGL
SRFRVNAYFQRSCISLAIRVVPTRIPALEELGLPPVLKKVAEKPQGLVLVTGPTGSGKSTTLASMIHYMNQTMRKHIITL
EDPIEYLHKHGNCIIDQREVGFDTKHFANGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTSSAPATIN
RIIDVFPPSQQAQIRIQLASVLVSIISQRLFLTADRKGRHAATEVLMNNSAVANLIRNEKIYQIINVMQTSRAQGMHTLE
TSITSLLESGKIAKESALPYIQEKVTDHAPL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=623892 LLY41_RS05795 WP_304587143.1 1165420..1166475(+) (pilT) [Cytobacillus firmus strain T8]
ATGAAGACAAAAATAGATTATCTGTTGAGGGCAGCGTATGAGCTGAAGGCTTCGGATCTGCATTTGACCGTTGGGGTTCC
TCCTGTCATACGGATTAATGGTGATTTAAAAAAGTATGGGAAAGATAGTTTGCGTCCGGAAGATACCGAGGGTATGGCTA
AAGCCATCATTCCTGCGAACATATGGAAGAAGTTTGAGGAGAACGGCGAGCTGGATTTTTCTTATGGAATACCCGGGTTA
TCCCGTTTCAGAGTCAACGCTTATTTTCAGCGGTCATGCATCTCACTGGCCATCCGTGTTGTTCCTACACGAATTCCTGC
TTTAGAAGAGCTTGGGCTTCCTCCTGTCTTAAAGAAAGTGGCGGAAAAGCCTCAGGGACTGGTCCTGGTGACAGGACCGA
CGGGAAGCGGAAAATCAACGACATTGGCTTCAATGATTCATTACATGAACCAGACGATGAGAAAGCACATTATTACGCTC
GAGGACCCGATAGAATATCTTCATAAGCATGGAAATTGTATTATTGACCAGCGGGAAGTTGGGTTTGATACAAAGCATTT
TGCAAATGGATTAAGAGCTGCTTTGCGCCAGGACCCGGATGTGATTCTTGTAGGGGAAATGCGTGACCTGGAAACCATCC
AGACAGCCATAACAGCGGCCGAAACTGGCCATTTGGTGCTTGGCACACTGCATACCTCAAGTGCTCCAGCTACGATTAAC
CGAATTATCGATGTATTTCCGCCCTCCCAGCAGGCGCAAATCAGAATACAGCTTGCTTCTGTATTAGTGTCGATTATATC
GCAGCGGCTTTTTCTCACAGCAGACAGGAAGGGCCGTCACGCAGCTACAGAAGTGCTTATGAATAATTCTGCTGTGGCAA
ACCTGATCAGAAATGAAAAGATCTACCAGATCATCAATGTGATGCAAACGTCACGTGCCCAGGGAATGCATACACTCGAG
ACAAGTATTACTTCTCTATTGGAGAGCGGAAAAATCGCCAAAGAATCAGCATTGCCGTATATACAGGAAAAGGTGACTGA
TCATGCCCCGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

96.866

0.484
  pilT Neisseria meningitidis 8013

49.102

95.157

0.467
  pilT Legionella pneumophila strain Lp02

48.949

94.872

0.464
  pilT Legionella pneumophila strain ERS1305867

48.949

94.872

0.464
  pilT Neisseria gonorrhoeae MS11

48.802

95.157

0.464
  pilT Vibrio cholerae strain A1552

49.085

93.447

0.459
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.085

93.447

0.459
  pilT Pseudomonas aeruginosa PAK

48.048

94.872

0.456
  pilT Pseudomonas stutzeri DSM 10701

47.147

94.872

0.447
  pilT Acinetobacter baylyi ADP1

47.256

93.447

0.442
  pilT Acinetobacter baumannii strain A118

46.246

94.872

0.439
  pilT Acinetobacter nosocomialis M2

46.246

94.872

0.439
  pilT Acinetobacter baumannii D1279779

46.246

94.872

0.439
  pilU Pseudomonas stutzeri DSM 10701

43.284

95.442

0.413
  pilU Acinetobacter baylyi ADP1

41.317

95.157

0.393
  pilU Vibrio cholerae strain A1552

42.105

92.023

0.387