Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LJY22_RS12210 Genome accession   NZ_CP085843
Coordinates   2670415..2671455 (-) Length   346 a.a.
NCBI ID   WP_005381015.1    Uniprot ID   A0A2I3C9Z7
Organism   Vibrio diabolicus strain NV27     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2665415..2676455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJY22_RS12180 (LJY22_12170) endA 2665568..2666263 (+) 696 WP_257896963.1 deoxyribonuclease I -
  LJY22_RS12185 (LJY22_12175) rsmE 2666380..2667111 (+) 732 WP_005393970.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LJY22_RS12190 (LJY22_12180) gshB 2667125..2668075 (+) 951 WP_006743090.1 glutathione synthase -
  LJY22_RS12195 (LJY22_12185) - 2668203..2668766 (+) 564 WP_005393973.1 YqgE/AlgH family protein -
  LJY22_RS12200 (LJY22_12190) ruvX 2668801..2669226 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  LJY22_RS12205 (LJY22_12195) pilU 2669288..2670394 (-) 1107 WP_006743091.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LJY22_RS12210 (LJY22_12200) pilT 2670415..2671455 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  LJY22_RS12215 (LJY22_12205) - 2671484..2672185 (+) 702 WP_134648191.1 YggS family pyridoxal phosphate-dependent enzyme -
  LJY22_RS12220 (LJY22_12210) proC 2672343..2673161 (+) 819 WP_047008834.1 pyrroline-5-carboxylate reductase -
  LJY22_RS12225 (LJY22_12215) - 2673214..2673771 (+) 558 WP_152811541.1 YggT family protein -
  LJY22_RS12230 (LJY22_12220) yggU 2673771..2674061 (+) 291 WP_005393980.1 DUF167 family protein YggU -
  LJY22_RS12235 (LJY22_12225) - 2674189..2674620 (+) 432 WP_005393982.1 DUF4426 domain-containing protein -
  LJY22_RS12240 (LJY22_12230) - 2674739..2675341 (+) 603 WP_046875112.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38532.02 Da        Isoelectric Point: 6.5039

>NTDB_id=620818 LJY22_RS12210 WP_005381015.1 2670415..2671455(-) (pilT) [Vibrio diabolicus strain NV27]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=620818 LJY22_RS12210 WP_005381015.1 2670415..2671455(-) (pilT) [Vibrio diabolicus strain NV27]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCAGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTGAGAAAACTAGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGACTTCTCTTTTGAGTTGCATAACGTTGGTCGT
TTCCGTGTCAACGCTTTCAACCAATCTCGTGGCTGTGCGGCAGTATTCCGTACCATTCCAAGTAGCATTCCAACTTTGGA
AGAGCTGGAAGCTCCCGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTGGTTACTGGCCCAACAGGTT
CTGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAATCGTAATCACAACAAGCACGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATCAACCAGCGTGAAGTACACCGTGATACGCACAGTTTCCAAAA
CGCACTGCGCAGCGCATTACGTGAAGACCCGGATGTCATTCTGGTTGGTGAGATGCGTGACAAAGAAACCATCAGCCTTG
CCTTAACGGCGGCAGAAACGGGGCACTTAGTTTTCGGGACGTTGCACACTAGCTCGGCGGCAAAAACCATTGACCGTATT
ATCGATGTGTTTCCTGGTAGTGATAAAGATATGGTCCGCTCGATGCTATCTGAGTCCCTTCGTTCGGTTATCGCGCAAAA
GCTTCTGAAGCGTAATGGTGGTGGCCGTATTGCATGCCATGAAATTATGATGGCAACGCCTGCGATTCGTAACTTGATTC
GCGAAGACAAAGTCGCACAGATGTATTCGATCATTCAAACAGGTGCGGCTCACGGCATGCAAACCATGGAGCAAAATGCT
CGTCAGTTAATGGCACAAGGTATGGTCGCTCGTGAAGAGGTCGATAGTAAGATCGGGTTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3C9Z7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

99.711

0.867
  pilT Vibrio cholerae strain A1552

86.957

99.711

0.867
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.429

95.087

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.399

0.671
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

91.908

0.486
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361