Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LJY22_RS12205 Genome accession   NZ_CP085843
Coordinates   2669288..2670394 (-) Length   368 a.a.
NCBI ID   WP_006743091.1    Uniprot ID   A0AAE4M7Q6
Organism   Vibrio diabolicus strain NV27     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2664288..2675394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJY22_RS12175 (LJY22_12165) - 2664920..2665417 (+) 498 WP_237312399.1 SprT family zinc-dependent metalloprotease -
  LJY22_RS12180 (LJY22_12170) endA 2665568..2666263 (+) 696 WP_257896963.1 deoxyribonuclease I -
  LJY22_RS12185 (LJY22_12175) rsmE 2666380..2667111 (+) 732 WP_005393970.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LJY22_RS12190 (LJY22_12180) gshB 2667125..2668075 (+) 951 WP_006743090.1 glutathione synthase -
  LJY22_RS12195 (LJY22_12185) - 2668203..2668766 (+) 564 WP_005393973.1 YqgE/AlgH family protein -
  LJY22_RS12200 (LJY22_12190) ruvX 2668801..2669226 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  LJY22_RS12205 (LJY22_12195) pilU 2669288..2670394 (-) 1107 WP_006743091.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LJY22_RS12210 (LJY22_12200) pilT 2670415..2671455 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  LJY22_RS12215 (LJY22_12205) - 2671484..2672185 (+) 702 WP_134648191.1 YggS family pyridoxal phosphate-dependent enzyme -
  LJY22_RS12220 (LJY22_12210) proC 2672343..2673161 (+) 819 WP_047008834.1 pyrroline-5-carboxylate reductase -
  LJY22_RS12225 (LJY22_12215) - 2673214..2673771 (+) 558 WP_152811541.1 YggT family protein -
  LJY22_RS12230 (LJY22_12220) yggU 2673771..2674061 (+) 291 WP_005393980.1 DUF167 family protein YggU -
  LJY22_RS12235 (LJY22_12225) - 2674189..2674620 (+) 432 WP_005393982.1 DUF4426 domain-containing protein -
  LJY22_RS12240 (LJY22_12230) - 2674739..2675341 (+) 603 WP_046875112.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41253.38 Da        Isoelectric Point: 6.7734

>NTDB_id=620817 LJY22_RS12205 WP_006743091.1 2669288..2670394(-) (pilU) [Vibrio diabolicus strain NV27]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=620817 LJY22_RS12205 WP_006743091.1 2669288..2670394(-) (pilU) [Vibrio diabolicus strain NV27]
ATGGATCTAAATAAATTTCTCGAAGGTATGCTGGCGCTGAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTTT
GTTTCGCGTGGATGGCGAATTGCGACCACAGGGTGAAAAACTTACCGAGAGTGATGTCGCGGCATTACTCGACAGCGCTA
TGGAGCCAGATCGGCGTCAGGAATTTCGTAAAAGTCGTGAGTCGAATTTTGCCATTGTGAGAGATTGTGGCCGCTTCCGT
GTTAGTGCCTTTTTTCAACGGGAATTACCGGGTGCGGTGATTCGTCGTATTGAAACCAATATTCCCACCTTTGAGCAATT
AAAGTTACCGTTAGTGTTGCAAGATTTAGCGATAGCCAAGCGTGGTTTGGTGCTGGTGGTTGGTGCTACTGGGTCAGGTA
AATCCACCACAATGGCGGCAATGACGGGCTATCGAAACAGCAACAAGACGGGACACATTTTGACGGTCGAAGATCCGATC
GAATTCGTGCATGAGCACAAGCGCTGTATCGTGACTCAACGCGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGTCAAGCGCCAGATATGATTTTGATTGGCGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CATTCGCTGAAACAGGTCACCTGTGTATGGCAACGTTGCACGCCAACAACGCCAACCAAGCCTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTTGATTTGTCGATGAACCTAAAAGGTGTGGTTGGTCAGCAGCTGAT
CCGAGATAAGAATGGGCAGGGGCGTCATGGTGTGTTTGAGATCTTGCTAAATAGCCCACGTGTGTCGGATTTGATCCGTC
GCGGAGATTTGCATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTTTAT
AAGTTGGTGATGCAAGGTAAGATCAGCGAAGAGGATGCGCTGCACAGTGCCGATTCGGCGAATGATTTGCGCTTGATGTT
GAAAACACAGCGTGGCGAAGCATTCTCTACGGGCAGTCTTGCTAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.609

100

0.826
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.692

89.946

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361