Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LIS82_RS20230 Genome accession   NZ_CP085712
Coordinates   3880127..3881167 (-) Length   346 a.a.
NCBI ID   WP_053477178.1    Uniprot ID   A0A0Q3TBV5
Organism   Cytobacillus solani strain F27     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3875127..3886167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS82_RS20205 (LIS82_20260) - 3875840..3876490 (-) 651 WP_252239243.1 hypothetical protein -
  LIS82_RS20210 (LIS82_20265) pilM 3876493..3877482 (-) 990 WP_252239244.1 pilus assembly protein PilM -
  LIS82_RS20215 (LIS82_20270) comC 3877497..3878258 (-) 762 WP_252239245.1 A24 family peptidase Machinery gene
  LIS82_RS20220 (LIS82_20275) - 3878322..3878789 (-) 468 WP_252239246.1 type II secretion system protein -
  LIS82_RS20225 (LIS82_20280) - 3878920..3880125 (-) 1206 WP_252239247.1 type II secretion system F family protein -
  LIS82_RS20230 (LIS82_20285) pilT 3880127..3881167 (-) 1041 WP_053477178.1 type IV pilus twitching motility protein PilT Machinery gene
  LIS82_RS20235 (LIS82_20290) - 3881179..3882840 (-) 1662 WP_056687258.1 ATPase, T2SS/T4P/T4SS family -
  LIS82_RS20240 (LIS82_20295) - 3882867..3884204 (-) 1338 WP_252239248.1 G5 domain-containing protein -
  LIS82_RS20245 (LIS82_20300) - 3884210..3885175 (-) 966 WP_252239249.1 hypothetical protein -
  LIS82_RS20250 (LIS82_20305) - 3885448..3885876 (+) 429 WP_056685883.1 type II secretion system protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38700.80 Da        Isoelectric Point: 7.6727

>NTDB_id=619499 LIS82_RS20230 WP_053477178.1 3880127..3881167(-) (pilT) [Cytobacillus solani strain F27]
MKEMIDHLLHAAFELKASDIHLTVGVPPIMRINGDLKKYGKESLKPEDTEGMARAIIPDHMWEQFKEKGELDFSYGIPKV
SRFRVNTYFQRSCIALAIRIVPTRIPTIEELELPNVLKRIAEKPQGLVLVTGPTGSGKSTTLASMIQYMNHHMRKHIITL
EDPIEYLHKHGNSIIDQREVGFDTNNFANGLRASLRQDPDVILVGEMRDLETIQTAITAAETGHLVFGTLHTSSAPATIN
RIIDVFPPSQQAQVRIQLASVLVSVISQRLFPTIDKKGRRAATEILINNSAVANLIRNEKIYQIVNIMQTSRASGMHTLE
TSIKELVQLGVISKEAAEPFIQEMLS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=619499 LIS82_RS20230 WP_053477178.1 3880127..3881167(-) (pilT) [Cytobacillus solani strain F27]
ATGAAAGAAATGATTGACCATTTGCTTCATGCAGCCTTTGAGCTGAAAGCATCAGATATTCATTTAACGGTGGGGGTACC
GCCGATTATGAGGATAAATGGGGATTTGAAGAAATATGGGAAGGAATCGCTGAAGCCGGAAGATACGGAAGGAATGGCAA
GAGCGATCATTCCTGATCATATGTGGGAGCAATTTAAAGAAAAAGGTGAATTAGATTTTTCCTATGGGATTCCGAAAGTA
TCCAGATTTCGCGTGAATACGTATTTCCAGCGGTCATGTATTGCCCTGGCAATTAGAATCGTTCCGACAAGAATACCGAC
AATAGAGGAATTGGAATTGCCGAATGTATTAAAGCGAATTGCTGAAAAACCACAAGGGCTTGTGTTGGTAACAGGCCCAA
CCGGGAGCGGAAAATCAACAACCTTGGCATCTATGATTCAATATATGAACCATCATATGAGAAAGCATATTATTACGCTG
GAAGATCCAATTGAGTATCTTCATAAACATGGAAATTCAATTATCGATCAGCGAGAAGTTGGTTTTGATACGAATAATTT
TGCCAATGGACTTAGAGCATCCTTAAGACAAGATCCTGATGTCATACTTGTTGGGGAAATGAGAGATTTAGAGACGATCC
AGACGGCGATCACTGCAGCCGAAACGGGGCATTTAGTTTTTGGAACCTTGCATACGTCAAGTGCGCCAGCAACAATCAAC
CGAATTATTGATGTTTTTCCGCCGTCTCAGCAGGCACAGGTTAGAATTCAATTGGCTTCTGTACTTGTATCCGTTATTTC
GCAGCGCTTATTTCCAACGATTGATAAAAAAGGGCGGAGAGCTGCAACTGAGATTTTGATTAACAACTCGGCTGTTGCTA
ATTTGATTCGCAATGAAAAAATCTATCAGATTGTTAATATTATGCAGACTTCAAGGGCATCTGGCATGCATACGCTTGAA
ACAAGCATCAAAGAACTTGTTCAATTAGGAGTTATTTCAAAAGAAGCAGCAGAACCATTCATACAGGAGATGCTGAGTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q3TBV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.853

97.977

0.488
  pilT Vibrio cholerae strain A1552

49.853

97.977

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.755

95.665

0.486
  pilT Neisseria meningitidis 8013

49.55

96.243

0.477
  pilT Neisseria gonorrhoeae MS11

49.55

96.243

0.477
  pilT Pseudomonas aeruginosa PAK

48.649

96.243

0.468
  pilT Legionella pneumophila strain Lp02

48.048

96.243

0.462
  pilT Legionella pneumophila strain ERS1305867

48.048

96.243

0.462
  pilT Acinetobacter baumannii strain A118

48.78

94.798

0.462
  pilT Acinetobacter nosocomialis M2

48.78

94.798

0.462
  pilT Acinetobacter baumannii D1279779

48.78

94.798

0.462
  pilT Acinetobacter baylyi ADP1

47.416

95.087

0.451
  pilT Pseudomonas stutzeri DSM 10701

46.108

96.532

0.445
  pilU Pseudomonas stutzeri DSM 10701

42.342

96.243

0.408
  pilU Vibrio cholerae strain A1552

42.547

93.064

0.396
  pilU Acinetobacter baylyi ADP1

42.5

92.486

0.393