Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VAPA_RS24445 Genome accession   NC_022247
Coordinates   5257066..5258109 (+) Length   347 a.a.
NCBI ID   WP_021012685.1    Uniprot ID   T1XIU5
Organism   Variovorax paradoxus B4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5252066..5263109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAPA_RS24425 (VAPA_1c49760) - 5252314..5252778 (+) 465 WP_021012681.1 hypothetical protein -
  VAPA_RS24430 (VAPA_1c49770) - 5252797..5255769 (-) 2973 WP_021012682.1 L-glutamate gamma-semialdehyde dehydrogenase -
  VAPA_RS24435 (VAPA_1c49780) - 5255858..5256331 (+) 474 WP_021012683.1 Lrp/AsnC ligand binding domain-containing protein -
  VAPA_RS24440 (VAPA_1c49790) - 5256328..5257014 (-) 687 WP_021012684.1 YggS family pyridoxal phosphate-dependent enzyme -
  VAPA_RS24445 (VAPA_1c49800) pilT 5257066..5258109 (+) 1044 WP_021012685.1 type IV pilus twitching motility protein PilT Machinery gene
  VAPA_RS24450 (VAPA_1c49810) pilU 5258137..5259273 (+) 1137 WP_021012686.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VAPA_RS24455 (VAPA_1c49820) - 5259317..5260225 (+) 909 WP_021012687.1 NAD(P)-dependent oxidoreductase -
  VAPA_RS24460 (VAPA_1c49830) - 5260263..5261024 (-) 762 WP_021012688.1 BON domain-containing protein -
  VAPA_RS24465 (VAPA_1c49840) - 5261021..5261665 (-) 645 WP_041946232.1 SIS domain-containing protein -
  VAPA_RS24470 (VAPA_1c49850) - 5261741..5262113 (-) 373 Protein_4940 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38127.82 Da        Isoelectric Point: 6.8885

>NTDB_id=61938 VAPA_RS24445 WP_021012685.1 5257066..5258109(+) (pilT) [Variovorax paradoxus B4]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKSVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFAELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSAIQTGQGSGMQTLDQN
LTDLVRRNAISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=61938 VAPA_RS24445 WP_021012685.1 5257066..5258109(+) (pilT) [Variovorax paradoxus B4]
GTGGACATTACCCAACTGCTGGCATTCAGCGTCAAGAACAAAGCCTCCGACCTGCATCTGTCGGCCGGCCTGCCGCCGAT
GATTCGCGTCCACGGCGACGTGCGGCGCATCAACGTCGATGCGCTCGACCACAAGTCGGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTATGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAATGCCTTCAACCAGGCGCGCGGCGCGGCGGCGGTGTTCCGGACCATTCCTTCGAAGATCCTCACGCTGGA
GCAGCTCAACGCGCCGAAGATTTTCGCGGAACTGGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACGGGGCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCCGCGATGGTCAACTACCTGAACGAAAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGTCCGATGACGCTCTCGTTCTCCAA
CGCCCTGCGCTCGGCGCTGCGCGAAGACCCGGACGCCATCCTGGTGGGCGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CCATGACCGCGGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAAAAGGAAATGATCCGCGCCATGCTGTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTGGACCAGAAC
CTGACCGACCTCGTGCGCCGCAATGCCATTTCGGCGGCGGAAGCACGGGGCAAGGCCAAGATCCCCGAGAATTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB T1XIU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395

Multiple sequence alignment