Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VAPA_RS24450 Genome accession   NC_022247
Coordinates   5258137..5259273 (+) Length   378 a.a.
NCBI ID   WP_021012686.1    Uniprot ID   T1XHN9
Organism   Variovorax paradoxus B4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5253137..5264273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAPA_RS24435 (VAPA_1c49780) - 5255858..5256331 (+) 474 WP_021012683.1 Lrp/AsnC ligand binding domain-containing protein -
  VAPA_RS24440 (VAPA_1c49790) - 5256328..5257014 (-) 687 WP_021012684.1 YggS family pyridoxal phosphate-dependent enzyme -
  VAPA_RS24445 (VAPA_1c49800) pilT 5257066..5258109 (+) 1044 WP_021012685.1 type IV pilus twitching motility protein PilT Machinery gene
  VAPA_RS24450 (VAPA_1c49810) pilU 5258137..5259273 (+) 1137 WP_021012686.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VAPA_RS24455 (VAPA_1c49820) - 5259317..5260225 (+) 909 WP_021012687.1 NAD(P)-dependent oxidoreductase -
  VAPA_RS24460 (VAPA_1c49830) - 5260263..5261024 (-) 762 WP_021012688.1 BON domain-containing protein -
  VAPA_RS24465 (VAPA_1c49840) - 5261021..5261665 (-) 645 WP_041946232.1 SIS domain-containing protein -
  VAPA_RS24470 (VAPA_1c49850) - 5261741..5262113 (-) 373 Protein_4940 YraN family protein -
  VAPA_RS24475 (VAPA_1c49860) rsmI 5262160..5263116 (+) 957 WP_021012691.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  VAPA_RS24480 (VAPA_1c49870) - 5263126..5263965 (-) 840 WP_021012692.1 IclR family transcriptional regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41884.90 Da        Isoelectric Point: 6.4189

>NTDB_id=61939 VAPA_RS24450 WP_021012686.1 5258137..5259273(+) (pilU) [Variovorax paradoxus B4]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVSMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLISQRLVPTEDGQGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSITFEDAIRNADSANDLRLQIKLNSQRARSTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=61939 VAPA_RS24450 WP_021012686.1 5258137..5259273(+) (pilU) [Variovorax paradoxus B4]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACTTGTTCAT
CACCGCCGACTTTCCGCCCGCGATCAAGGTCGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGGGCGCAGCACA
CGCTGGCGCTCACGCGCTCGGTCATGAACGACCGCCAGACGGCGGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCGACGGGCATCGGCCGCTTCCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATCCC
GGCCAAGCTGCCGACCATCGACGGGCTGGGCATGCCGCAGGTGCTGAAGGACGTGTCGATGACCAAGCGCGGCCTCACCA
TCCTGGTGGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCCATGATCGACTGGCGCAACGAAAACTCCTACGGC
CACATCGTGACGGTGGAAGATCCGGTCGAGTTCGTGCATCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCGGTGGCCTTTGCCGAAACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAGGAGCGCCGCGCCCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGTTCGCTGATCTCCCAGCGCCTGGTGCCCACCGAGGACGGCCAGGGCCGCGTGGCGGCAGTGGAAGTGCTGCTGAACA
CGCCGCTCATTTCGGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGAGGAAGAGCCGCAACCTTGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAGCCACTCGATCACCTTCGAGGACGCCATCCGCAACGCCGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGTCGGCCGGCACGGAGC
ATTTCGCGATTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB T1XHN9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter baumannii D1279779

44.828

84.392

0.378
  pilT Acinetobacter baumannii strain A118

44.828

84.392

0.378
  pilT Acinetobacter baylyi ADP1

42.643

88.095

0.376
  pilT Legionella pneumophila strain Lp02

43.161

87.037

0.376
  pilT Legionella pneumophila strain ERS1305867

43.161

87.037

0.376

Multiple sequence alignment