Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HWQ47_RS07970 Genome accession   NZ_CP085636
Coordinates   1899744..1900781 (+) Length   345 a.a.
NCBI ID   WP_269970625.1    Uniprot ID   -
Organism   Shewanella sp. MTB7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1894744..1905781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ47_RS07935 (HWQ47_07940) hemW 1894761..1895897 (-) 1137 WP_269970619.1 radical SAM family heme chaperone HemW -
  HWQ47_RS07940 (HWQ47_07945) rdgB 1895897..1896499 (-) 603 WP_269970620.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  HWQ47_RS07945 (HWQ47_07950) - 1896640..1897074 (-) 435 WP_269970621.1 DUF4426 domain-containing protein -
  HWQ47_RS07950 (HWQ47_07955) yggU 1897147..1897434 (-) 288 WP_269970622.1 DUF167 family protein YggU -
  HWQ47_RS07955 (HWQ47_07960) - 1897434..1897982 (-) 549 WP_269970623.1 YggT family protein -
  HWQ47_RS07960 (HWQ47_07965) proC 1898042..1898860 (-) 819 WP_269970624.1 pyrroline-5-carboxylate reductase -
  HWQ47_RS07965 (HWQ47_07970) - 1899006..1899707 (-) 702 WP_269971695.1 YggS family pyridoxal phosphate-dependent enzyme -
  HWQ47_RS07970 (HWQ47_07975) pilT 1899744..1900781 (+) 1038 WP_269970625.1 type IV pilus twitching motility protein PilT Machinery gene
  HWQ47_RS07975 (HWQ47_07980) pilU 1900810..1901922 (+) 1113 WP_269970626.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HWQ47_RS07980 (HWQ47_07985) hemH 1902156..1903187 (+) 1032 WP_269970627.1 ferrochelatase -
  HWQ47_RS07985 (HWQ47_07990) ruvX 1903232..1903651 (-) 420 WP_269970628.1 Holliday junction resolvase RuvX -
  HWQ47_RS07990 (HWQ47_07995) - 1903735..1904295 (-) 561 WP_269970629.1 YqgE/AlgH family protein -
  HWQ47_RS07995 (HWQ47_08000) yciH 1904333..1904662 (-) 330 WP_269970630.1 stress response translation initiation inhibitor YciH -
  HWQ47_RS08000 (HWQ47_08005) - 1904826..1905140 (-) 315 WP_269970631.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38418.94 Da        Isoelectric Point: 6.8235

>NTDB_id=618998 HWQ47_RS07970 WP_269970625.1 1899744..1900781(+) (pilT) [Shewanella sp. MTB7]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGDVRKINLPALDHQAVHGLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQARGAAAVFRTIPSDILSLEQLGAPEIFKRISEFPRGLVLVTGPTGSGKSTTLAAMVDYINESRHEHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGQITREDAQHKSSNKQTTF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=618998 HWQ47_RS07970 WP_269970625.1 1899744..1900781(+) (pilT) [Shewanella sp. MTB7]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAACACAAAGCGTCAGATTTACATCTCTCTGCAGGTGTGTCTCCTAT
GATACGTGTTGATGGCGATGTCAGAAAGATCAATTTGCCCGCTTTAGATCATCAGGCAGTGCATGGACTTGTCTATGACA
TCATGAATGATAAGCAGCGTAAAGATTATGAAGAGCATTTAGAGATAGATTTTTCATTTGAAGTGCCTAACCTTGCACGC
TTTCGAGTCAACGCTTTTAACCAAGCCAGAGGAGCAGCTGCAGTATTTCGTACCATTCCCAGCGACATCTTGAGCCTTGA
GCAACTTGGCGCACCGGAAATATTTAAGAGAATATCTGAATTCCCCCGTGGTTTGGTGCTGGTAACCGGCCCGACAGGTT
CAGGTAAAAGTACCACGCTTGCGGCCATGGTTGATTATATCAATGAAAGCCGGCATGAACACATTCTCACCATCGAAGAC
CCGATAGAATTTGTGCATCAGAATAAGCAGTGTTTGATTAACCAACGAGAGGTTCATCGTCATACTCATAGCTTTAATGC
TGCACTTAGAAGTGCACTTCGTGAAGACCCTGATGTGATCTTAGTGGGTGAGATGCGAGACTTGGAAACCATACGTTTGG
CAATGACGGCGGCAGAAACGGGTCATTTAGTTTTTGGTACCTTACATACCACCTCTGCAGCTAAAACCATTGACCGTGTT
GTGGATGTATTTCCTGAAGGAGAAAAAGGAATGGTACGTACTATGTTATCTGAGTCACTTCAGGCGGTAATTTCACAAAC
CTTGATCAAAAAGGTTGGTGGTGGTCGAGTGGCAGCTCATGAGATCATGATGGGCACGCCTGCAATTCGAAACTTAATTC
GCGAAGACAAAGTGGCACAGATGTATTCAGCAATTCAAACTGGTATGGCTCACGGTATGCAAACATTAGATCAATGTCTG
CAAAATTTGGTTAATCGAGGCCAAATCACCCGTGAAGATGCTCAACATAAGAGCTCCAATAAGCAAACTACGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

78.717

99.42

0.783
  pilT Acinetobacter nosocomialis M2

78.717

99.42

0.783
  pilT Acinetobacter baumannii strain A118

78.717

99.42

0.783
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.193

99.13

0.765
  pilT Acinetobacter baylyi ADP1

79.635

95.362

0.759
  pilT Legionella pneumophila strain Lp02

75.367

98.841

0.745
  pilT Legionella pneumophila strain ERS1305867

75.367

98.841

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Vibrio cholerae strain A1552

40.762

98.841

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

39.583

97.391

0.386