Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HWQ47_RS07975 Genome accession   NZ_CP085636
Coordinates   1900810..1901922 (+) Length   370 a.a.
NCBI ID   WP_269970626.1    Uniprot ID   -
Organism   Shewanella sp. MTB7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1895810..1906922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ47_RS07940 (HWQ47_07945) rdgB 1895897..1896499 (-) 603 WP_269970620.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  HWQ47_RS07945 (HWQ47_07950) - 1896640..1897074 (-) 435 WP_269970621.1 DUF4426 domain-containing protein -
  HWQ47_RS07950 (HWQ47_07955) yggU 1897147..1897434 (-) 288 WP_269970622.1 DUF167 family protein YggU -
  HWQ47_RS07955 (HWQ47_07960) - 1897434..1897982 (-) 549 WP_269970623.1 YggT family protein -
  HWQ47_RS07960 (HWQ47_07965) proC 1898042..1898860 (-) 819 WP_269970624.1 pyrroline-5-carboxylate reductase -
  HWQ47_RS07965 (HWQ47_07970) - 1899006..1899707 (-) 702 WP_269971695.1 YggS family pyridoxal phosphate-dependent enzyme -
  HWQ47_RS07970 (HWQ47_07975) pilT 1899744..1900781 (+) 1038 WP_269970625.1 type IV pilus twitching motility protein PilT Machinery gene
  HWQ47_RS07975 (HWQ47_07980) pilU 1900810..1901922 (+) 1113 WP_269970626.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HWQ47_RS07980 (HWQ47_07985) hemH 1902156..1903187 (+) 1032 WP_269970627.1 ferrochelatase -
  HWQ47_RS07985 (HWQ47_07990) ruvX 1903232..1903651 (-) 420 WP_269970628.1 Holliday junction resolvase RuvX -
  HWQ47_RS07990 (HWQ47_07995) - 1903735..1904295 (-) 561 WP_269970629.1 YqgE/AlgH family protein -
  HWQ47_RS07995 (HWQ47_08000) yciH 1904333..1904662 (-) 330 WP_269970630.1 stress response translation initiation inhibitor YciH -
  HWQ47_RS08000 (HWQ47_08005) - 1904826..1905140 (-) 315 WP_269970631.1 hypothetical protein -
  HWQ47_RS08005 (HWQ47_08010) - 1905312..1905905 (+) 594 WP_269970632.1 DUF4136 domain-containing protein -
  HWQ47_RS08010 (HWQ47_08015) - 1906152..1906604 (+) 453 WP_269970633.1 DUF924 family protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40986.90 Da        Isoelectric Point: 6.0089

>NTDB_id=618999 HWQ47_RS07975 WP_269970626.1 1900810..1901922(+) (pilU) [Shewanella sp. MTB7]
MDVRPFLKIMVERKASDLFITAGFPPSAKIDGELRPLGENSFTPAQSLDFVESLMSDVQKKEFHETRECNFAFAVKDLGR
FRVSAFWQRESPGCVMRRIETNIPDVDDLNLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNANSRGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALAFAETGHLCMATLHANNANQALDRI
MNLVPQNKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEILINTPRISSLIAKNELHSLKETMSKSNEQGMQTFDQA
LLKLYVEGEISYADALHHADSPNDLRLMIKLQSSDTGGSGFMEGVTLDLN

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=618999 HWQ47_RS07975 WP_269970626.1 1900810..1901922(+) (pilU) [Shewanella sp. MTB7]
ATGGATGTTCGTCCCTTTTTAAAAATAATGGTGGAACGCAAAGCGTCGGATCTGTTTATTACGGCTGGTTTTCCACCCAG
TGCAAAGATAGACGGTGAGTTAAGGCCTTTAGGTGAAAACTCTTTTACGCCAGCGCAATCGTTAGATTTCGTCGAGTCGT
TAATGAGCGATGTGCAAAAAAAAGAGTTCCACGAGACCCGTGAGTGTAACTTTGCTTTTGCGGTAAAAGATTTGGGACGT
TTTCGTGTGAGTGCATTTTGGCAAAGGGAATCTCCTGGTTGCGTTATGCGTCGTATCGAAACCAATATTCCCGATGTCGA
TGACTTAAATCTTCCTCCTATTTTGAAAGATTTGGTAATGAGTAAGCGTGGACTCATCATCATGGTCGGAGGAACCGGCA
CCGGTAAATCAACCTCATTAGCTGCTCTTGTTGGTTATCGCAATGCCAATTCTCGCGGACATATTCTTACCATTGAAGAT
CCAGTCGAGTTTGTTCATGATCATCGTAAGAGTATTATTACTCAGCGAGAAGTGGGGATCGATACCGAATCCTTTGATGC
GGCACTTAAAAGTTCTCTGCGTCAGGCTCCTGATGTGATTTTAATTGGTGAGATTCGAAGCCAAGAGACGATGGAGTTTG
CATTGGCTTTCGCGGAAACTGGGCACCTATGTATGGCGACGCTTCATGCCAATAATGCAAACCAGGCTCTGGATCGTATT
ATGAACTTAGTGCCTCAAAATAAGCATCAACAATTACTATTTGACCTTTCGCTTAACTTGCGTGGTATTGTCGCTCAGCA
ACTTGTTCCTAAAGCCGATGGTACTGGGCGTCGAGCGGCGATTGAGATTTTAATTAACACGCCAAGGATCAGTAGTCTTA
TCGCTAAGAATGAACTTCATTCTCTTAAAGAGACCATGTCTAAGTCAAATGAACAGGGGATGCAAACTTTCGATCAGGCA
TTATTGAAGCTCTATGTTGAAGGCGAGATTAGTTATGCAGATGCTTTGCATCATGCTGATTCACCAAATGATCTGCGTTT
GATGATCAAACTGCAAAGTTCAGACACTGGTGGTTCAGGCTTTATGGAGGGAGTCACGTTAGATCTTAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.215

100

0.605
  pilU Vibrio cholerae strain A1552

58.266

99.73

0.581
  pilU Acinetobacter baylyi ADP1

60.511

95.135

0.576
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.39

97.027

0.392
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

89.189

0.381
  pilT Vibrio cholerae strain A1552

42.727

89.189

0.381
  pilT Legionella pneumophila strain Lp02

41.994

89.459

0.376
  pilT Legionella pneumophila strain ERS1305867

41.994

89.459

0.376
  pilT Acinetobacter baumannii D1279779

40.351

92.432

0.373
  pilT Acinetobacter baumannii strain A118

40.351

92.432

0.373
  pilT Acinetobacter nosocomialis M2

40.351

92.432

0.373
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362