Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LCH97_RS13365 Genome accession   NZ_CP085530
Coordinates   2790998..2792035 (-) Length   345 a.a.
NCBI ID   WP_017507798.1    Uniprot ID   -
Organism   Vogesella sp. XCS3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2785998..2797035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH97_RS13340 (LCH97_13340) - 2786076..2786819 (+) 744 WP_017507793.1 amino acid ABC transporter permease -
  LCH97_RS13345 (LCH97_13345) - 2786819..2787484 (+) 666 WP_370630698.1 amino acid ABC transporter permease -
  LCH97_RS13350 (LCH97_13350) - 2787487..2788221 (+) 735 WP_026107760.1 amino acid ABC transporter ATP-binding protein -
  LCH97_RS13355 (LCH97_13355) - 2788569..2789780 (+) 1212 WP_227302123.1 EAL and HDOD domain-containing protein -
  LCH97_RS13360 (LCH97_13360) pilU 2789849..2790985 (-) 1137 WP_017507797.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LCH97_RS13365 (LCH97_13365) pilT 2790998..2792035 (-) 1038 WP_017507798.1 type IV pilus twitching motility protein PilT Machinery gene
  LCH97_RS13370 (LCH97_13370) - 2792154..2792846 (+) 693 WP_227302124.1 YggS family pyridoxal phosphate-dependent enzyme -
  LCH97_RS13375 (LCH97_13375) proC 2792898..2793698 (+) 801 WP_227302125.1 pyrroline-5-carboxylate reductase -
  LCH97_RS13380 (LCH97_13380) - 2793701..2794282 (+) 582 WP_227302126.1 YggT family protein -
  LCH97_RS13385 (LCH97_13385) - 2794349..2794654 (+) 306 WP_227302127.1 c-type cytochrome -
  LCH97_RS13390 (LCH97_13390) dksA 2794870..2795280 (+) 411 WP_026107763.1 RNA polymerase-binding protein DksA -
  LCH97_RS13395 (LCH97_13395) aroG 2795413..2796486 (+) 1074 WP_017507804.1 3-deoxy-7-phosphoheptulonate synthase AroG -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38400.95 Da        Isoelectric Point: 6.8739

>NTDB_id=618867 LCH97_RS13365 WP_017507798.1 2790998..2792035(-) (pilT) [Vogesella sp. XCS3]
MEISDLLAFTVKNKASDLHLSAGLPPMIRVHGDIRRINLPPLAHHDVHDMVYDIMNDYQRKIFEDTYEADFSFEVPGVAR
FRVNVFVQNRGIGAVFRVIPSRVLTLEDLGAPRIFKEISDYPRGIVLVTGPTGSGKSTTLAAMIDYINANHYSHILTVED
PIEFVHESKKSLVNQRELGSQTRSFSAALRSALREDPDVILIGEMRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKEMVRSMLSESLRAVISQSLLKTKDESARIAAHEIMIGTPAIRNLIRENKIAQINSMIQTGQQHGMQTLDQC
LQELVRHGHVSVQEARSKASNKDAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=618867 LCH97_RS13365 WP_017507798.1 2790998..2792035(-) (pilT) [Vogesella sp. XCS3]
ATGGAAATATCTGATCTTCTGGCCTTTACGGTCAAGAACAAAGCCTCTGACCTGCATTTATCGGCCGGCCTGCCGCCGAT
GATACGCGTGCATGGCGATATCCGCCGCATCAACCTGCCACCGCTGGCGCACCACGATGTGCACGACATGGTGTACGACA
TCATGAACGACTACCAGCGCAAGATTTTTGAAGACACCTACGAAGCAGACTTCTCTTTCGAAGTGCCGGGCGTGGCGCGT
TTCCGGGTAAACGTCTTCGTGCAGAACCGTGGTATCGGCGCGGTATTCCGCGTGATTCCCTCCCGCGTACTGACGCTGGA
AGACCTGGGCGCGCCGCGCATCTTCAAGGAAATTTCCGACTACCCGCGCGGCATCGTGCTGGTGACCGGCCCCACCGGCT
CGGGTAAATCCACCACGCTGGCGGCGATGATCGACTACATCAACGCCAACCACTACTCGCACATCCTGACGGTAGAAGAC
CCGATCGAATTCGTGCACGAGAGCAAGAAGTCGCTGGTAAACCAGCGTGAACTGGGCTCGCAGACGCGCAGCTTTTCGGC
GGCGCTGCGTAGCGCACTGCGCGAAGACCCGGACGTGATCCTGATCGGTGAAATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGTCGGCCGCAGAAACCGGCCACCTGGTATTCGGCACCCTGCACACCAGCAGTGCGGCCAAAACCATTGACCGTATC
GTGGACGTGTTCCCGGCCGGCGAAAAGGAAATGGTGCGCTCCATGCTGTCGGAAAGCCTGCGCGCGGTGATTTCGCAAAG
CCTGCTGAAAACCAAGGACGAAAGCGCCCGTATTGCCGCGCACGAGATCATGATCGGTACCCCGGCCATCCGTAACCTGA
TCCGCGAAAACAAGATCGCGCAGATCAACTCCATGATCCAGACCGGCCAGCAGCACGGCATGCAGACGCTGGACCAGTGC
CTGCAGGAGCTGGTACGCCACGGCCATGTTTCGGTGCAGGAAGCACGAAGCAAAGCCAGTAACAAGGACGCTTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

73.913

100

0.739
  pilT Acinetobacter baumannii D1279779

73.913

100

0.739
  pilT Acinetobacter baumannii strain A118

73.913

100

0.739
  pilT Pseudomonas aeruginosa PAK

73.333

100

0.733
  pilT Pseudomonas stutzeri DSM 10701

72.464

100

0.725
  pilT Acinetobacter baylyi ADP1

71.014

100

0.71
  pilT Neisseria meningitidis 8013

70.435

100

0.704
  pilT Neisseria gonorrhoeae MS11

70.145

100

0.701
  pilT Legionella pneumophila strain Lp02

69.855

100

0.699
  pilT Legionella pneumophila strain ERS1305867

69.855

100

0.699
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.793

97.971

0.635
  pilT Vibrio cholerae strain A1552

64.793

97.971

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.504
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Acinetobacter baylyi ADP1

41.176

98.551

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4