Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LCH97_RS13360 Genome accession   NZ_CP085530
Coordinates   2789849..2790985 (-) Length   378 a.a.
NCBI ID   WP_017507797.1    Uniprot ID   -
Organism   Vogesella sp. XCS3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2784849..2795985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH97_RS13335 (LCH97_13335) - 2785058..2785954 (+) 897 WP_227302122.1 glutamate/aspartate ABC transporter substrate-binding protein -
  LCH97_RS13340 (LCH97_13340) - 2786076..2786819 (+) 744 WP_017507793.1 amino acid ABC transporter permease -
  LCH97_RS13345 (LCH97_13345) - 2786819..2787484 (+) 666 WP_370630698.1 amino acid ABC transporter permease -
  LCH97_RS13350 (LCH97_13350) - 2787487..2788221 (+) 735 WP_026107760.1 amino acid ABC transporter ATP-binding protein -
  LCH97_RS13355 (LCH97_13355) - 2788569..2789780 (+) 1212 WP_227302123.1 EAL and HDOD domain-containing protein -
  LCH97_RS13360 (LCH97_13360) pilU 2789849..2790985 (-) 1137 WP_017507797.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LCH97_RS13365 (LCH97_13365) pilT 2790998..2792035 (-) 1038 WP_017507798.1 type IV pilus twitching motility protein PilT Machinery gene
  LCH97_RS13370 (LCH97_13370) - 2792154..2792846 (+) 693 WP_227302124.1 YggS family pyridoxal phosphate-dependent enzyme -
  LCH97_RS13375 (LCH97_13375) proC 2792898..2793698 (+) 801 WP_227302125.1 pyrroline-5-carboxylate reductase -
  LCH97_RS13380 (LCH97_13380) - 2793701..2794282 (+) 582 WP_227302126.1 YggT family protein -
  LCH97_RS13385 (LCH97_13385) - 2794349..2794654 (+) 306 WP_227302127.1 c-type cytochrome -
  LCH97_RS13390 (LCH97_13390) dksA 2794870..2795280 (+) 411 WP_026107763.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42006.19 Da        Isoelectric Point: 6.2894

>NTDB_id=618866 LCH97_RS13360 WP_017507797.1 2789849..2790985(-) (pilU) [Vogesella sp. XCS3]
MEKDQASKFIHDLLKHAISKNASDIFISADFPPAMKIDGKITPVAPQPLTAQHTKELVRSVMNDRQTEEFESTREANFAI
SPPGIGRFRVSAYVQQGSAGMVLRKINTEIPTLDGLGLPDVLKDIALIKRGLVIFVGGTGSGKSTSLAALVDWRNSTTAD
HIITIEDPIEYVHNHKKSIITQREIGVDTESWEIALKNTLRQAPDVILMGEIRDRESMMYGLQFAETGHLCLATLHANNA
NQALDRILNFFPEERHQQVLMDLSLNMRAIISQRLVPLKGSKGRTAAIEILLNSPLISDMIFKGEISGIKEVMGRSKESG
MQTFDQALFQLFEAGLVSYEDALRNADSINDLRLQIKLYSEKAKQHNPLDGLDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=618866 LCH97_RS13360 WP_017507797.1 2789849..2790985(-) (pilU) [Vogesella sp. XCS3]
ATGGAAAAAGACCAGGCATCAAAATTCATTCACGACCTGCTGAAGCACGCCATCAGCAAGAACGCTTCCGATATTTTCAT
CAGCGCCGACTTCCCGCCGGCCATGAAGATAGACGGCAAGATTACCCCGGTAGCGCCCCAGCCACTGACTGCCCAGCACA
CCAAAGAGCTGGTGCGCTCGGTGATGAACGACCGCCAGACCGAAGAGTTTGAATCCACCCGCGAAGCCAACTTTGCCATC
AGCCCGCCGGGTATCGGCCGCTTCCGCGTCAGCGCCTACGTGCAGCAAGGCAGTGCCGGCATGGTGCTGCGCAAGATCAA
CACCGAAATCCCCACCCTGGACGGCCTGGGCCTGCCGGACGTGCTGAAAGACATCGCACTGATCAAACGCGGCCTGGTGA
TCTTTGTGGGCGGTACCGGCTCGGGTAAATCCACCTCGCTGGCCGCCCTGGTGGACTGGCGCAACAGCACCACAGCCGAC
CACATCATCACCATCGAAGACCCGATCGAGTACGTCCACAACCACAAGAAGAGCATCATTACGCAGCGCGAGATCGGCGT
GGATACCGAAAGCTGGGAAATAGCGCTGAAAAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAATCCATGATGTACGGCCTGCAGTTTGCCGAAACCGGCCACCTGTGCCTGGCCACGCTACACGCCAACAACGCC
AACCAGGCGCTGGACCGCATCCTGAACTTCTTCCCGGAAGAGCGTCACCAGCAAGTGCTGATGGACTTGTCGCTGAACAT
GCGCGCCATCATCTCGCAGCGCCTGGTTCCGCTCAAAGGCAGCAAGGGCCGCACGGCTGCCATCGAAATCCTGCTGAACA
GCCCGCTGATCTCCGACATGATCTTCAAAGGCGAAATCAGCGGCATCAAGGAAGTCATGGGCCGCTCGAAGGAGTCTGGT
ATGCAGACATTCGACCAGGCGCTATTCCAGCTGTTCGAAGCCGGCCTGGTGAGCTACGAAGACGCGCTGCGCAACGCCGA
CTCCATCAACGACCTGCGCCTGCAGATCAAGCTGTACAGCGAAAAAGCCAAGCAGCACAACCCGCTGGACGGGCTGGACC
ATCTGGACATCGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.017

93.651

0.571
  pilU Acinetobacter baylyi ADP1

56.131

97.09

0.545
  pilU Vibrio cholerae strain A1552

52.422

92.857

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

89.153

0.399