Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LEO80_RS18650 Genome accession   NZ_CP085468
Coordinates   4007950..4008984 (+) Length   344 a.a.
NCBI ID   WP_010673050.1    Uniprot ID   A0A081LS93
Organism   Aeromonas caviae strain 71485     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4002950..4013984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO80_RS18610 (LEO80_18610) - 4003167..4003541 (+) 375 WP_039039964.1 YacL family protein -
  LEO80_RS18615 (LEO80_18615) - 4003591..4003881 (+) 291 WP_039039963.1 hypothetical protein -
  LEO80_RS18620 (LEO80_18620) - 4004043..4004873 (+) 831 WP_042864458.1 CPBP family intramembrane glutamic endopeptidase -
  LEO80_RS18625 (LEO80_18625) - 4004949..4005368 (-) 420 WP_227263065.1 DUF4426 domain-containing protein -
  LEO80_RS18630 (LEO80_18630) yggU 4005387..4005686 (-) 300 WP_227263066.1 DUF167 family protein YggU -
  LEO80_RS18635 (LEO80_18635) - 4005686..4006237 (-) 552 WP_010673053.1 YggT family protein -
  LEO80_RS18640 (LEO80_18640) proC 4006261..4007085 (-) 825 WP_109112045.1 pyrroline-5-carboxylate reductase -
  LEO80_RS18645 (LEO80_18645) - 4007209..4007910 (-) 702 WP_042014924.1 YggS family pyridoxal phosphate-dependent enzyme -
  LEO80_RS18650 (LEO80_18650) pilT 4007950..4008984 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  LEO80_RS18655 (LEO80_18655) pilU 4009008..4010117 (+) 1110 WP_201926733.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LEO80_RS18660 (LEO80_18660) yaaA 4010150..4010923 (+) 774 WP_201926731.1 peroxide stress protein YaaA -
  LEO80_RS18665 (LEO80_18665) srmB 4011112..4012332 (-) 1221 WP_227263067.1 ATP-dependent RNA helicase SrmB -
  LEO80_RS18670 (LEO80_18670) - 4012460..4013170 (+) 711 WP_227263068.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38189.90 Da        Isoelectric Point: 6.7436

>NTDB_id=618215 LEO80_RS18650 WP_010673050.1 4007950..4008984(+) (pilT) [Aeromonas caviae strain 71485]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLDDLGAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGVVASLDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=618215 LEO80_RS18650 WP_010673050.1 4007950..4008984(+) (pilT) [Aeromonas caviae strain 71485]
ATGGATATCACAGAGTTATTGGCATTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCGGCCGGGGTCCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGGGAAGTCCATGCCCTCATTTACGACA
TCATGAATGACCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTGGACTTCTCCTTCGAAGTACCCGGCATGGCACGC
TTTCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCCGTGTTTCGTACCATCCCGAGCACCGTTCTGACCCTGGA
CGATCTGGGGGCGCCGGAGATATTTCGCAAGATAGCGGAGTACCCTCGCGGCCTGGTGCTGGTGACTGGGCCCACCGGCT
CGGGCAAATCCACCACCCTGGCCGCCATGGTCGATTACATCAATGACAACTTCCACCACCACATCCTCACCATCGAGGAC
CCCATTGAATTCGTGCACGAGAACAAGCGCTGCTTGGTGAACCAGCGGGAAGTACACCGCGACACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGATATCATCCTGGTAGGTGAAATGCGGGACCTCGAAACCATCCGTCTCG
CCATGACCGCCGCCGAAACCGGTCACCTGGTGTTTGGCACCCTGCACACCTCCTCGGCCGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGCTGGGGATCCCGGCGGTGCGAAACCTCATCC
GGGAAGACAAGGTGGCCCAGCTCTATTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGACCAGAGCCTC
AAGCAGCTGGTCAACCGGGGGGTCGTCGCCTCCCTTGATGCCAAGGCCAAGGCGGTCGATCCCAATACCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081LS93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Pseudomonas stutzeri DSM 10701

78.299

99.128

0.776
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Pseudomonas aeruginosa PAK

76.833

99.128

0.762
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

71.471

98.837

0.706
  pilT Legionella pneumophila strain ERS1305867

71.471

98.837

0.706
  pilT Neisseria meningitidis 8013

69.027

98.547

0.68
  pilT Neisseria gonorrhoeae MS11

68.732

98.547

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.924

99.419

0.526
  pilU Vibrio cholerae strain A1552

43.188

100

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407