Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LEO80_RS18655 Genome accession   NZ_CP085468
Coordinates   4009008..4010117 (+) Length   369 a.a.
NCBI ID   WP_201926733.1    Uniprot ID   -
Organism   Aeromonas caviae strain 71485     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4004008..4015117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO80_RS18620 (LEO80_18620) - 4004043..4004873 (+) 831 WP_042864458.1 CPBP family intramembrane glutamic endopeptidase -
  LEO80_RS18625 (LEO80_18625) - 4004949..4005368 (-) 420 WP_227263065.1 DUF4426 domain-containing protein -
  LEO80_RS18630 (LEO80_18630) yggU 4005387..4005686 (-) 300 WP_227263066.1 DUF167 family protein YggU -
  LEO80_RS18635 (LEO80_18635) - 4005686..4006237 (-) 552 WP_010673053.1 YggT family protein -
  LEO80_RS18640 (LEO80_18640) proC 4006261..4007085 (-) 825 WP_109112045.1 pyrroline-5-carboxylate reductase -
  LEO80_RS18645 (LEO80_18645) - 4007209..4007910 (-) 702 WP_042014924.1 YggS family pyridoxal phosphate-dependent enzyme -
  LEO80_RS18650 (LEO80_18650) pilT 4007950..4008984 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  LEO80_RS18655 (LEO80_18655) pilU 4009008..4010117 (+) 1110 WP_201926733.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LEO80_RS18660 (LEO80_18660) yaaA 4010150..4010923 (+) 774 WP_201926731.1 peroxide stress protein YaaA -
  LEO80_RS18665 (LEO80_18665) srmB 4011112..4012332 (-) 1221 WP_227263067.1 ATP-dependent RNA helicase SrmB -
  LEO80_RS18670 (LEO80_18670) - 4012460..4013170 (+) 711 WP_227263068.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  LEO80_RS18675 (LEO80_18675) brnQ 4013406..4014668 (+) 1263 WP_050485178.1 branched-chain amino acid transport system II carrier protein -
  LEO80_RS18680 (LEO80_18680) - 4014759..4014986 (-) 228 WP_128344179.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40901.00 Da        Isoelectric Point: 6.1841

>NTDB_id=618216 LEO80_RS18655 WP_201926733.1 4009008..4010117(+) (pilU) [Aeromonas caviae strain 71485]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPVPLDNTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=618216 LEO80_RS18655 WP_201926733.1 4009008..4010117(+) (pilU) [Aeromonas caviae strain 71485]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCTCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGACCAGTCCCCCTCGACAATACGACGGCGCTGGCGTTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAGCGTTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACCGGTG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTATCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379