Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LEO77_RS02680 Genome accession   NZ_CP085465
Coordinates   565839..566873 (+) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain 71506     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 560839..571873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO77_RS02650 (LEO77_02650) - 561506..562702 (+) 1197 WP_227341896.1 NupC/NupG family nucleoside CNT transporter -
  LEO77_RS02655 (LEO77_02655) - 562804..563223 (-) 420 WP_005340339.1 DUF4426 domain-containing protein -
  LEO77_RS02660 (LEO77_02660) yggU 563267..563569 (-) 303 WP_005340341.1 DUF167 family protein YggU -
  LEO77_RS02665 (LEO77_02665) - 563569..564120 (-) 552 WP_047436998.1 YggT family protein -
  LEO77_RS02670 (LEO77_02670) proC 564132..564956 (-) 825 WP_201990756.1 pyrroline-5-carboxylate reductase -
  LEO77_RS02675 (LEO77_02675) - 565098..565799 (-) 702 WP_100654584.1 YggS family pyridoxal phosphate-dependent enzyme -
  LEO77_RS02680 (LEO77_02680) pilT 565839..566873 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  LEO77_RS02685 (LEO77_02685) pilU 566902..568011 (+) 1110 WP_227342977.1 type IVa pilus ATPase TapU Machinery gene
  LEO77_RS02690 (LEO77_02690) yaaA 568059..568832 (+) 774 WP_201937767.1 peroxide stress protein YaaA -
  LEO77_RS02695 (LEO77_02695) - 569027..569452 (+) 426 WP_005340360.1 hypothetical protein -
  LEO77_RS02700 (LEO77_02700) - 569812..570303 (-) 492 WP_227341897.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  LEO77_RS02705 (LEO77_02705) arfB 570329..570745 (-) 417 WP_139430011.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  LEO77_RS02710 (LEO77_02710) glnK 570947..571285 (+) 339 WP_005341406.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=618177 LEO77_RS02680 WP_005340354.1 565839..566873(+) (pilT) [Aeromonas veronii strain 71506]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=618177 LEO77_RS02680 WP_005340354.1 565839..566873(+) (pilT) [Aeromonas veronii strain 71506]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGATTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGTTCGGGCGCGGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTCCGCAAGATTGCCGAGTTCCCGCGGGGTCTGGTGTTGGTGACCGGCCCGACCGGTT
CCGGTAAGTCGACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATCCTCACCATCGAGGAC
CCCATCGAATTCGTCCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTACACCGTGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTCTGG
CCATGACTGCCGCCGAAACCGGCCACTTGGTGTTTGGCACTCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCTGGTGCGGAGAAGGACATGGTACGCTCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATTGGTGGCGGTCGGGTGGCAGCGCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTCATTC
GGGAGGACAAGATTGCCCAGCTTTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGTGGCGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCCGTTGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407