Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LEO77_RS02685 Genome accession   NZ_CP085465
Coordinates   566902..568011 (+) Length   369 a.a.
NCBI ID   WP_227342977.1    Uniprot ID   -
Organism   Aeromonas veronii strain 71506     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 561902..573011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO77_RS02655 (LEO77_02655) - 562804..563223 (-) 420 WP_005340339.1 DUF4426 domain-containing protein -
  LEO77_RS02660 (LEO77_02660) yggU 563267..563569 (-) 303 WP_005340341.1 DUF167 family protein YggU -
  LEO77_RS02665 (LEO77_02665) - 563569..564120 (-) 552 WP_047436998.1 YggT family protein -
  LEO77_RS02670 (LEO77_02670) proC 564132..564956 (-) 825 WP_201990756.1 pyrroline-5-carboxylate reductase -
  LEO77_RS02675 (LEO77_02675) - 565098..565799 (-) 702 WP_100654584.1 YggS family pyridoxal phosphate-dependent enzyme -
  LEO77_RS02680 (LEO77_02680) pilT 565839..566873 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  LEO77_RS02685 (LEO77_02685) pilU 566902..568011 (+) 1110 WP_227342977.1 type IVa pilus ATPase TapU Machinery gene
  LEO77_RS02690 (LEO77_02690) yaaA 568059..568832 (+) 774 WP_201937767.1 peroxide stress protein YaaA -
  LEO77_RS02695 (LEO77_02695) - 569027..569452 (+) 426 WP_005340360.1 hypothetical protein -
  LEO77_RS02700 (LEO77_02700) - 569812..570303 (-) 492 WP_227341897.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  LEO77_RS02705 (LEO77_02705) arfB 570329..570745 (-) 417 WP_139430011.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  LEO77_RS02710 (LEO77_02710) glnK 570947..571285 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  LEO77_RS02715 (LEO77_02715) - 571544..572551 (+) 1008 WP_021230948.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41010.92 Da        Isoelectric Point: 6.2350

>NTDB_id=618178 LEO77_RS02685 WP_227342977.1 566902..568011(+) (pilU) [Aeromonas veronii strain 71506]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGDALDKKGALTLVRDTLSSDHFERYVRTKEANYAIYREALGR
FRVSAFWQQELPGMVIRRIETRIPTFEDLQLPRILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLVPSVDGLRRSAAFEILLNTPLITDIIRKGEMHRLKEVMTKSAELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLENVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=618178 LEO77_RS02685 WP_227342977.1 566902..568011(+) (pilU) [Aeromonas veronii strain 71506]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTAAATGGCCATCTGGTGTCGCTGGGGGGAGACGCGCTCGACAAGAAAGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATGTCCGCACCAAGGAGGCCAACTACGCGATTTACCGCGAGGCGCTGGGTCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGTATGGTGATTCGTCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTCCCCAGGATCCTGCAGGAGGTGGCGATGGCCAAGCGCGGGCTGGTGCTGTTTGTCGGTGCCACCGGGG
CGGGTAAATCGACCACTCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCATATCCTGACGGTGGAAGAC
CCGGTGGAGTTCGTCCATCAGCATGGCCGTAGCCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGAGTCGTTCGATGT
AGCACTGAAAAGCTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTTCAGTTCGCCGAGACTGGCCATCTCTGTCTCGCCACTTTGCATGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGAGAAACATCGCCAGTTTCTGTTCGATCTCTCCTTCAACCTCAGAGCCATTGTCGCCCAGCA
ACTGGTGCCGAGTGTCGATGGTTTGCGGCGCAGTGCGGCATTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATCA
TCCGCAAGGGAGAGATGCACCGTCTCAAGGAGGTGATGACCAAATCTGCCGAACTGGGCATGCAGACCTTCGATCAGGCG
CTTTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCCCATGCCGACTCGGCCAACGACCTGCGGCT
GCTGATCAAGCTCTCCGGTCGCGAGCAGCTGGGGTCCGGCACACTGGAAAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Legionella pneumophila strain ERS1305867

41.108

92.954

0.382
  pilT Legionella pneumophila strain Lp02

41.108

92.954

0.382
  pilT Vibrio cholerae strain A1552

40.719

90.515

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.515

0.369
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.118

91.87

0.369
  pilT Acinetobacter baylyi ADP1

39.589

92.412

0.366