Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LIN44_RS04325 Genome accession   NZ_CP085344
Coordinates   914198..915241 (+) Length   347 a.a.
NCBI ID   WP_025583874.1    Uniprot ID   A0A375BRE3
Organism   Cupriavidus sp. MP-37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 909198..920241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIN44_RS04300 (LIN44_04300) - 909297..910739 (+) 1443 WP_227313648.1 catalase -
  LIN44_RS04305 (LIN44_04305) - 910884..911369 (+) 486 WP_012353810.1 Dps family protein -
  LIN44_RS04310 (LIN44_04310) ubiA 911511..912368 (+) 858 WP_227313649.1 4-hydroxybenzoate octaprenyltransferase -
  LIN44_RS04315 (LIN44_04315) proC 912562..913398 (-) 837 WP_227313650.1 pyrroline-5-carboxylate reductase -
  LIN44_RS04320 (LIN44_04320) - 913442..914131 (-) 690 WP_227313651.1 YggS family pyridoxal phosphate-dependent enzyme -
  LIN44_RS04325 (LIN44_04325) pilT 914198..915241 (+) 1044 WP_025583874.1 type IV pilus twitching motility protein PilT Machinery gene
  LIN44_RS04330 (LIN44_04330) pilU 915277..916416 (+) 1140 WP_092306471.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LIN44_RS04335 (LIN44_04335) - 916471..916965 (+) 495 WP_112774296.1 glutathione peroxidase -
  LIN44_RS04340 (LIN44_04340) - 916969..917658 (-) 690 WP_227313652.1 YafY family protein -
  LIN44_RS04345 (LIN44_04345) - 917681..918052 (-) 372 WP_227313653.1 VOC family protein -
  LIN44_RS04350 (LIN44_04350) - 918177..918902 (-) 726 WP_082819011.1 LrgB family protein -
  LIN44_RS04355 (LIN44_04355) - 918899..919282 (-) 384 WP_227313654.1 CidA/LrgA family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38394.18 Da        Isoelectric Point: 6.9772

>NTDB_id=617226 LIN44_RS04325 WP_025583874.1 914198..915241(+) (pilT) [Cupriavidus sp. MP-37]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVASMTHKDVHAMVYDIMSDTQRKAYEERLEIDFSFEIAGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNENDMGHILTVED
PIEFVHSSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRSVISYNDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=617226 LIN44_RS04325 WP_025583874.1 914198..915241(+) (pilT) [Cupriavidus sp. MP-37]
ATGGACATCGCGCAGCTATTGGCTTTCGCCGTCAAGAACAAGGCGTCCGATCTTCATCTGTCCGCGGACATGCCGCCGAT
GGTGCGGATCCACGGCGACATGCGCCGCATCAACGTCGCCTCGATGACGCACAAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACACCCAGCGCAAGGCCTACGAGGAACGCCTCGAAATCGATTTCTCGTTCGAGATCGCCGGCCTGTCGCGC
TTCCGCGTCAATGCCTATAACACCCAGCGCGGCGCCGCCGCGGTGTTCCGTACCATTCCGTCCAAGGTGCTGACGCTGGA
AGAGCTGCGCGCGCCGGCGGTATTTGCCGACCTGTGCATGAAGCCGCGCGGGCTGGTGCTGGTAACGGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGGTCGACCATCGCAATGAAAACGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACAGCTCCAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGGCCGCATACGCATTCGTTCGCCAA
CGCGCTGCGCTCGGCGCTGCGCGAAGACCCTGACGTGATCCTGGTGGGTGAACTGCGCGACCTCGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTCTTCGGCACGCTGCACACCAGTTCGGCGGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGCCCGAAGAGAAGGACATGGTGCGCACCATGCTGTCGGAATCGCTCGAGGCGGTGATCTCGCAGAC
GCTGCTGAAGACCCGCGACGGCAACGGCCGCACCGCCGCGCACGAGATCATGATCGCCACCCCGGCGATCCGCCACCTGA
TCCGCGAAAACAAGATCGCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGTCGGACCTGATCAAGCGCAGCGTCATCAGCTACAACGACGCGCGCGCGATTGCCAAGAATCCGGACGCGTTCATGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A375BRE3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.965

99.712

0.718
  pilT Acinetobacter baumannii D1279779

71.965

99.712

0.718
  pilT Acinetobacter baumannii strain A118

71.965

99.712

0.718
  pilT Pseudomonas stutzeri DSM 10701

69.855

99.424

0.695
  pilT Pseudomonas aeruginosa PAK

69.855

99.424

0.695
  pilT Acinetobacter baylyi ADP1

69.565

99.424

0.692
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Legionella pneumophila strain Lp02

66.957

99.424

0.666
  pilT Legionella pneumophila strain ERS1305867

66.957

99.424

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.172

97.118

0.643
  pilT Vibrio cholerae strain A1552

66.172

97.118

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

99.424

0.493
  pilU Acinetobacter baylyi ADP1

40.116

99.135

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392