Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LIN44_RS04330 Genome accession   NZ_CP085344
Coordinates   915277..916416 (+) Length   379 a.a.
NCBI ID   WP_092306471.1    Uniprot ID   A0A375E0P6
Organism   Cupriavidus sp. MP-37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 910277..921416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIN44_RS04305 (LIN44_04305) - 910884..911369 (+) 486 WP_012353810.1 Dps family protein -
  LIN44_RS04310 (LIN44_04310) ubiA 911511..912368 (+) 858 WP_227313649.1 4-hydroxybenzoate octaprenyltransferase -
  LIN44_RS04315 (LIN44_04315) proC 912562..913398 (-) 837 WP_227313650.1 pyrroline-5-carboxylate reductase -
  LIN44_RS04320 (LIN44_04320) - 913442..914131 (-) 690 WP_227313651.1 YggS family pyridoxal phosphate-dependent enzyme -
  LIN44_RS04325 (LIN44_04325) pilT 914198..915241 (+) 1044 WP_025583874.1 type IV pilus twitching motility protein PilT Machinery gene
  LIN44_RS04330 (LIN44_04330) pilU 915277..916416 (+) 1140 WP_092306471.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LIN44_RS04335 (LIN44_04335) - 916471..916965 (+) 495 WP_112774296.1 glutathione peroxidase -
  LIN44_RS04340 (LIN44_04340) - 916969..917658 (-) 690 WP_227313652.1 YafY family protein -
  LIN44_RS04345 (LIN44_04345) - 917681..918052 (-) 372 WP_227313653.1 VOC family protein -
  LIN44_RS04350 (LIN44_04350) - 918177..918902 (-) 726 WP_082819011.1 LrgB family protein -
  LIN44_RS04355 (LIN44_04355) - 918899..919282 (-) 384 WP_227313654.1 CidA/LrgA family protein -
  LIN44_RS04360 (LIN44_04360) glcF 919444..920697 (-) 1254 WP_227313655.1 glycolate oxidase subunit GlcF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42178.38 Da        Isoelectric Point: 6.6918

>NTDB_id=617227 LIN44_RS04330 WP_092306471.1 915277..916416(+) (pilU) [Cupriavidus sp. MP-37]
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKITPVSQQPLNPTQALGLVRSVMNERQVQDFDTSRECNFA
ISAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVADLDLPQTLHDVVMAKRGLVIVTGATGSGKSTTLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFDLYEQGKITYEDALRNADSLNDLRLMIKLHSTHAKDSDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=617227 LIN44_RS04330 WP_092306471.1 915277..916416(+) (pilU) [Cupriavidus sp. MP-37]
ATGCTCGACCGCGAATCCGCCGCCAAGTACATCAACGACCTGCTCGAGCTGATGGTCAGCAACCGCGGCTCGGACCTGTT
CATCACTTCGGACTTTCCGCCGGCGATCAAGGTCGATGGCAAGATCACGCCGGTGTCGCAGCAGCCGCTGAACCCGACCC
AGGCGCTGGGCCTGGTGCGCTCGGTGATGAACGAGCGCCAGGTGCAGGACTTCGACACCAGCCGCGAATGCAATTTCGCG
ATCAGCGCGCCCAAGGCCGGGCGCTTCCGCGTCTCGGCCTTTATCCAGCAGGGCAAGGCCGGCATGGTGGTGCGCACCAT
CAATACGCGCATCCCGTCGGTGGCCGACCTGGACCTGCCGCAGACGCTGCATGACGTGGTGATGGCCAAGCGCGGGCTGG
TGATCGTCACCGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCAATGCTCGACCACCGCAACGCGCATTCCTAC
GGCCATATCATCACCATCGAGGATCCGATCGAATACGTGCACGCGCACCAGAACTGCATCGTCACGCAGCGCGAGGTCGG
CATCGATACCGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAGATCC
GCGACCGCGAGACCATGGAGTACGCGATGCAGTACGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCCAACCAGGCGATCGACCGCGTGGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAGCTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGATCTCGCAGCGCCTGCTGCCGCGCGCGGGCCGCAAGGGCCGGGTGCCGGCGGTCGAGATCATGATCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCACGAGCTCAAGGAAGTCATCAAGAAGTCGCGCGAGCAG
GGCATGATCTCGTTCGACCAGGCGCTGTTCGACCTGTACGAGCAGGGCAAGATCACCTACGAGGACGCGCTGCGCAACGC
CGACTCGCTCAACGACCTGCGCCTGATGATCAAGCTGCACAGCACGCATGCCAAGGACTCGGACCTGGGCGCCGGCACCG
AGCATCTCAACGTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A375E0P6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.506

91.821

0.583
  pilU Acinetobacter baylyi ADP1

57.714

92.348

0.533
  pilU Vibrio cholerae strain A1552

53.444

95.778

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

88.918

0.404
  pilT Pseudomonas stutzeri DSM 10701

43.323

88.918

0.385
  pilT Pseudomonas aeruginosa PAK

43.323

88.918

0.385
  pilT Acinetobacter baumannii D1279779

43.544

87.863

0.383
  pilT Acinetobacter nosocomialis M2

43.544

87.863

0.383
  pilT Acinetobacter baumannii strain A118

43.544

87.863

0.383
  pilT Acinetobacter baylyi ADP1

42.773

89.446

0.383
  pilT Legionella pneumophila strain Lp02

42.042

87.863

0.369
  pilT Legionella pneumophila strain ERS1305867

42.042

87.863

0.369
  pilT Neisseria meningitidis 8013

40.588

89.71

0.364