Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG313_RS01325 Genome accession   NZ_CP085273
Coordinates   301953..302993 (-) Length   346 a.a.
NCBI ID   WP_404341032.1    Uniprot ID   -
Organism   Pseudoalteromonas mariniglutinosa strain ABH-469     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 296953..307993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG313_RS01300 (LG313_01300) gshB 297731..298684 (+) 954 WP_404341026.1 glutathione synthase -
  LG313_RS01305 (LG313_01305) - 298901..299458 (+) 558 WP_404341028.1 YqgE/AlgH family protein -
  LG313_RS01310 (LG313_01310) ruvX 299479..299928 (+) 450 WP_404341029.1 Holliday junction resolvase RuvX -
  LG313_RS01315 (LG313_01315) - 299925..300800 (-) 876 WP_404341030.1 hypothetical protein -
  LG313_RS01320 (LG313_01320) pilU 300802..301932 (-) 1131 WP_404341031.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG313_RS01325 (LG313_01325) pilT 301953..302993 (-) 1041 WP_404341032.1 type IV pilus twitching motility protein PilT Machinery gene
  LG313_RS01330 (LG313_01330) - 302996..303709 (+) 714 WP_404341034.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG313_RS01335 (LG313_01335) proC 303725..304546 (+) 822 WP_404341035.1 pyrroline-5-carboxylate reductase -
  LG313_RS01340 (LG313_01340) - 304573..305106 (+) 534 WP_404341036.1 YggT family protein -
  LG313_RS01345 (LG313_01345) - 305160..305603 (+) 444 WP_404341037.1 DUF4426 domain-containing protein -
  LG313_RS01350 (LG313_01350) - 305613..306221 (+) 609 WP_404341038.1 XTP/dITP diphosphatase -
  LG313_RS01355 (LG313_01355) hemW 306218..307354 (+) 1137 WP_404341039.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38280.57 Da        Isoelectric Point: 5.9604

>NTDB_id=616795 LG313_RS01325 WP_404341032.1 301953..302993(-) (pilT) [Pseudoalteromonas mariniglutinosa strain ABH-469]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALEQKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSDVLSLDDLGAPDIFKTIADNPRGLVLVTGPTGSGKSTTLAAMVDYINQNKHHHILTIED
PIEFVHENKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMVRSMLSESLRAVISQSLLKKISGGRIAAHEIMLGIPAIRNLIREDKIAQMYSAIQTGASIGMQTMDQCL
TSLINRGIVTTDEARTKAQDKSQFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=616795 LG313_RS01325 WP_404341032.1 301953..302993(-) (pilT) [Pseudoalteromonas mariniglutinosa strain ABH-469]
ATGGATATTACCGAATTATTGGCGTTTAGTGTGCAACACAAAGCGTCAGATTTACATTTATCATCGGGCGTTTCTCCGAT
GATCCGCGTTGATGGCGATGTGCGTCGTATTAATATTCCAGCATTAGAACAAAAAGATGTAAATAGTCTGGTCTACGATA
TTATGAATGATAATCAACGAAAGGACTACGAACAAAACTTAGAAGTTGACTTTTCATTCGAAGTCCCCAATCTTGCACGT
TTCCGTGTTAATGCATTTAATTCAAATCGTGGCCCTGCGGCAGTGTTTCGGACAATTCCCAGTGATGTACTGAGTTTAGA
TGATTTAGGAGCACCTGATATATTTAAAACTATTGCCGACAATCCCCGAGGTTTAGTGCTTGTTACGGGCCCTACTGGTT
CGGGTAAGTCTACGACTCTTGCTGCTATGGTTGATTATATTAATCAAAATAAGCATCACCATATTTTAACGATTGAAGAT
CCGATAGAGTTTGTGCATGAAAACAAACTGAGTTTAATTAATCAGCGTGAGGTACATCGCGATACACACAGTTTCTCTAA
CGCGCTTCGAAGTGCATTACGTGAGGATCCTGATGTAATTTTGGTTGGTGAGTTGCGCGATCTAGAAACAATTCGTCTTG
CGATGACTGCTGCTGAAACGGGACATCTGGTATTTGGCACTTTGCATACTACTTCAGCACCAAAAACCATCGACCGTATT
ATTGATGTATTTCCTGGTGAAGAAAAAGACATGGTACGCTCGATGTTATCTGAATCGCTTCGTGCCGTTATTTCACAAAG
CCTGCTAAAAAAAATCAGTGGCGGACGTATTGCGGCTCATGAAATCATGTTGGGTATTCCTGCAATTAGAAATTTGATCC
GTGAAGATAAAATTGCGCAGATGTACTCAGCCATTCAAACTGGCGCGTCAATTGGGATGCAAACCATGGATCAATGTTTA
ACCAGTTTAATTAATCGTGGCATAGTCACAACGGATGAGGCGCGGACGAAAGCGCAGGATAAAAGCCAGTTTGGTGGTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.744

99.422

0.763
  pilT Acinetobacter baumannii strain A118

76.744

99.422

0.763
  pilT Acinetobacter nosocomialis M2

76.744

99.422

0.763
  pilT Acinetobacter baylyi ADP1

75

99.422

0.746
  pilT Pseudomonas stutzeri DSM 10701

75

99.422

0.746
  pilT Pseudomonas aeruginosa PAK

74.709

99.422

0.743
  pilT Legionella pneumophila strain Lp02

72.674

99.422

0.723
  pilT Legionella pneumophila strain ERS1305867

72.674

99.422

0.723
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

97.977

0.699
  pilT Vibrio cholerae strain A1552

71.386

97.977

0.699
  pilT Neisseria meningitidis 8013

70.381

98.555

0.694
  pilT Neisseria gonorrhoeae MS11

70.088

98.555

0.691
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.711

0.52
  pilU Vibrio cholerae strain A1552

42.154

93.931

0.396
  pilU Acinetobacter baylyi ADP1

39.118

98.266

0.384
  pilU Pseudomonas stutzeri DSM 10701

39.104

96.821

0.379