Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LG313_RS01320 Genome accession   NZ_CP085273
Coordinates   300802..301932 (-) Length   376 a.a.
NCBI ID   WP_404341031.1    Uniprot ID   -
Organism   Pseudoalteromonas mariniglutinosa strain ABH-469     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 295802..306932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG313_RS01290 (LG313_01290) - 296408..296875 (+) 468 WP_404341024.1 hypothetical protein -
  LG313_RS01295 (LG313_01295) rsmE 296939..297673 (+) 735 WP_404341025.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LG313_RS01300 (LG313_01300) gshB 297731..298684 (+) 954 WP_404341026.1 glutathione synthase -
  LG313_RS01305 (LG313_01305) - 298901..299458 (+) 558 WP_404341028.1 YqgE/AlgH family protein -
  LG313_RS01310 (LG313_01310) ruvX 299479..299928 (+) 450 WP_404341029.1 Holliday junction resolvase RuvX -
  LG313_RS01315 (LG313_01315) - 299925..300800 (-) 876 WP_404341030.1 hypothetical protein -
  LG313_RS01320 (LG313_01320) pilU 300802..301932 (-) 1131 WP_404341031.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG313_RS01325 (LG313_01325) pilT 301953..302993 (-) 1041 WP_404341032.1 type IV pilus twitching motility protein PilT Machinery gene
  LG313_RS01330 (LG313_01330) - 302996..303709 (+) 714 WP_404341034.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG313_RS01335 (LG313_01335) proC 303725..304546 (+) 822 WP_404341035.1 pyrroline-5-carboxylate reductase -
  LG313_RS01340 (LG313_01340) - 304573..305106 (+) 534 WP_404341036.1 YggT family protein -
  LG313_RS01345 (LG313_01345) - 305160..305603 (+) 444 WP_404341037.1 DUF4426 domain-containing protein -
  LG313_RS01350 (LG313_01350) - 305613..306221 (+) 609 WP_404341038.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41835.78 Da        Isoelectric Point: 6.1873

>NTDB_id=616794 LG313_RS01320 WP_404341031.1 300802..301932(-) (pilU) [Pseudoalteromonas mariniglutinosa strain ABH-469]
MSLSLNSFLQILIDKQGSDLFVSSQLPVSAKINGELQPLSDEKLTDEQSLAMVESAMNEKQRAEYHQTKECNFAIATEQG
RFRVSAFWQRDRAGMVIRRIVTQIPDVNELGLPSVLTDVIMSKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIE
DPIEFVHEHRKSIVTQREVGLDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLLPTADGEGRVAAIEILLNSPMVAELIKKGDIGSIKETMAKSKEMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLQNNEQQGAGFLQGVTIDGLDDKGR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=616794 LG313_RS01320 WP_404341031.1 300802..301932(-) (pilU) [Pseudoalteromonas mariniglutinosa strain ABH-469]
ATGTCTTTATCGTTAAATTCTTTTCTACAGATCTTGATTGATAAACAAGGATCTGACTTGTTTGTCTCTAGTCAATTGCC
CGTGAGTGCGAAAATCAATGGTGAATTACAGCCGCTGAGTGACGAAAAGCTGACAGATGAACAATCATTGGCGATGGTTG
AATCGGCAATGAATGAAAAGCAGCGAGCAGAGTATCATCAGACTAAAGAGTGTAATTTCGCAATTGCCACAGAGCAAGGA
CGTTTTCGTGTTTCAGCATTTTGGCAACGTGATCGCGCAGGCATGGTTATTCGTCGAATTGTCACCCAAATTCCCGATGT
TAATGAACTGGGTTTACCTTCTGTTTTAACCGATGTGATCATGTCAAAACGTGGCTTAGTTTTATTTGTTGGCGGAACGG
GAACGGGTAAGTCAACGTCACTGGCTGCTTTGCTGGGCTATCGTAACCGAAACCAGCGCGGCCATATTCTAACCATTGAA
GATCCTATTGAATTTGTTCATGAGCACCGAAAAAGTATTGTGACGCAACGTGAAGTTGGATTAGATACGGATAGTTTTGA
GTCAGCGTTAAAAAGTTCATTACGCCAAGCGCCTGATGTGATCCTGATCGGTGAGATCCGTTCGCAAGAAACCATGGAAT
ATGCTTTGAGTTTTGCTGAAACCGGCCATTTATGTGTTGCGACCCTGCATGCGAATAATGCCAATCAGGCGATTGATCGA
ATTATGCATTTAGTTCCTAAGGAGAAACATGACAAGTTGAAATATGACTTGGCTTTGAATTTAAGGGCAATTATTGCCCA
ACAACTACTGCCTACGGCGGACGGAGAGGGGCGTGTTGCAGCAATTGAAATTTTGCTTAATTCTCCGATGGTCGCTGAAC
TGATCAAAAAAGGCGATATTGGAAGTATCAAAGAAACCATGGCGAAATCGAAAGAAATGGGCATGCAAACCTTTGATCAG
GCACTGTTTGAATTATACAGGCAACAACGTATTAATTATGCAGATGCTCTTCACCATGCTGACTCACCAAACGATTTACG
ATTGATGATTAAACTACAAAATAACGAACAACAAGGAGCCGGTTTTTTACAAGGCGTTACTATTGATGGCCTAGATGATA
AAGGTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.614

100

0.569
  pilU Vibrio cholerae strain A1552

56.676

97.606

0.553
  pilU Acinetobacter baylyi ADP1

55.172

100

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.5

95.745

0.407
  pilT Acinetobacter baylyi ADP1

40.058

90.957

0.364
  pilT Pseudomonas stutzeri DSM 10701

40.719

88.83

0.362
  pilT Pseudomonas aeruginosa PAK

40.719

88.83

0.362