Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG286_RS01825 Genome accession   NZ_CP085265
Coordinates   384453..385496 (+) Length   347 a.a.
NCBI ID   WP_404344647.1    Uniprot ID   -
Organism   Vreelandella venusta strain ABH-461     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 379453..390496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG286_RS01795 (LG286_01790) - 379729..380190 (-) 462 WP_404344642.1 DUF4426 domain-containing protein -
  LG286_RS01800 (LG286_01795) metW 380246..380842 (-) 597 WP_404344643.1 methionine biosynthesis protein MetW -
  LG286_RS01805 (LG286_01800) - 380842..382038 (-) 1197 WP_404344644.1 homoserine O-acetyltransferase -
  LG286_RS01810 (LG286_01805) - 382112..382705 (-) 594 WP_311065758.1 YggT family protein -
  LG286_RS01815 (LG286_01810) proC 382799..383632 (-) 834 WP_404344645.1 pyrroline-5-carboxylate reductase -
  LG286_RS01820 (LG286_01815) - 383721..384419 (-) 699 WP_404344646.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG286_RS01825 (LG286_01820) pilT 384453..385496 (+) 1044 WP_404344647.1 type IV pilus twitching motility protein PilT Machinery gene
  LG286_RS01830 (LG286_01825) pilU 385521..386684 (+) 1164 WP_404344648.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG286_RS01835 (LG286_01830) - 386697..387839 (-) 1143 WP_404344649.1 anhydro-N-acetylmuramic acid kinase -
  LG286_RS01840 (LG286_01835) tyrS 387956..389155 (+) 1200 WP_404344650.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38247.83 Da        Isoelectric Point: 6.5962

>NTDB_id=616712 LG286_RS01825 WP_404344647.1 384453..385496(+) (pilT) [Vreelandella venusta strain ABH-461]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMENSDVRRLIYDIMNDRQRRDYEEHLEADFSFEVPGVAR
FRVNAFNQARGAGAVFRTIPNKVLSMETLGMGEVFQRLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINEQRFEHILTIED
PVEFVHTSKRCLINQREVHRDTHSFSGALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKSMVRSMLSESLQAVVSQTLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQIYSAIQTGGALGMQTLNASL
AKLVKEGVVSMEEAQARAKGTLTLKDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=616712 LG286_RS01825 WP_404344647.1 384453..385496(+) (pilT) [Vreelandella venusta strain ABH-461]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAATGCATCCGACCTGCACCTTTCGGCGGGTCTGCCTCCCAT
GATACGTGTTGATGGCGATATTCGTCGGCTTAATGTGCCCGCCATGGAGAACAGCGACGTTCGGCGGCTGATCTACGACA
TCATGAACGATCGGCAGCGCCGCGACTACGAAGAGCACTTAGAAGCAGACTTCTCGTTTGAGGTGCCGGGGGTGGCGCGC
TTTAGGGTTAATGCCTTCAACCAGGCACGCGGGGCGGGGGCGGTGTTCCGTACCATTCCCAATAAGGTGCTTTCCATGGA
AACTCTGGGCATGGGCGAAGTGTTCCAGCGCCTTTCGATGCTACCCAGGGGATTAGTGCTAGTGACCGGGCCCACGGGGT
CGGGCAAAAGCACCACCTTGGCGGCGATGATCGACTACATCAACGAACAGCGCTTCGAGCATATTCTGACCATCGAAGAT
CCGGTGGAGTTTGTGCACACCAGCAAACGCTGCCTGATCAATCAGCGGGAGGTTCACCGCGACACCCATAGCTTCTCTGG
GGCGCTGCGTAGTGCGCTGCGCGAAGACCCGGACGTGATTTTGGTCGGCGAGCTGCGGGATCTCGAAACGATCCGTTTAG
CGCTCACCGCTGCTGAAACGGGGCATTTGGTATTCGGCACCCTGCACACCACTTCCGCGGCGAAAACCATCGACCGGATT
ATTGATGTGTTCCCCGGCGAAGAGAAATCCATGGTGCGCTCGATGCTGTCTGAATCGCTCCAGGCGGTTGTCTCGCAAAC
GTTGTTGAAGCGCCAGGGTGGCGGGCGCGTGGCGGCCCACGAGATCCTGATTGCCACGTCTGCGGTGCGTAACCTCATTC
GCGAAGATAAGGTGGCGCAGATCTACTCCGCCATCCAGACCGGCGGCGCACTGGGAATGCAGACCCTGAATGCCTCGCTC
GCCAAGCTGGTTAAAGAGGGCGTGGTGAGTATGGAGGAAGCCCAAGCAAGGGCTAAAGGCACGCTGACGTTAAAAGATGA
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

79.646

97.695

0.778
  pilT Pseudomonas stutzeri DSM 10701

79.056

97.695

0.772
  pilT Acinetobacter baumannii D1279779

77.876

97.695

0.761
  pilT Acinetobacter baumannii strain A118

77.876

97.695

0.761
  pilT Acinetobacter nosocomialis M2

77.876

97.695

0.761
  pilT Acinetobacter baylyi ADP1

75.221

97.695

0.735
  pilT Legionella pneumophila strain Lp02

71.893

97.406

0.7
  pilT Legionella pneumophila strain ERS1305867

71.893

97.406

0.7
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.92

97.118

0.689
  pilT Vibrio cholerae strain A1552

70.92

97.118

0.689
  pilT Neisseria meningitidis 8013

67.544

98.559

0.666
  pilT Neisseria gonorrhoeae MS11

67.251

98.559

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.687

97.695

0.524
  pilU Pseudomonas stutzeri DSM 10701

40.751

99.712

0.406
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392
  pilU Acinetobacter baylyi ADP1

39.193

100

0.392