Detailed information NTG0616713

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LG286_RS01830
• Genome accession: NZ_CP085265
• Coordinates: 385521..386684 (+)
• Length: 387 a.a.
• NCBI ID: WP_404344648.1
• Uniprot ID: -
• Organism: Vreelandella venusta strain ABH-461
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 380521..391684

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LG286_RS01805 (LG286_01800)	-	380842..382038 (-)	1197	WP_404344644.1	homoserine O-acetyltransferase	-
LG286_RS01810 (LG286_01805)	-	382112..382705 (-)	594	WP_311065758.1	YggT family protein	-
LG286_RS01815 (LG286_01810)	proC	382799..383632 (-)	834	WP_404344645.1	pyrroline-5-carboxylate reductase	-
LG286_RS01820 (LG286_01815)	-	383721..384419 (-)	699	WP_404344646.1	YggS family pyridoxal phosphate-dependent enzyme	-
LG286_RS01825 (LG286_01820)	pilT	384453..385496 (+)	1044	WP_404344647.1	type IV pilus twitching motility protein PilT	Machinery gene
LG286_RS01830 (LG286_01825)	pilU	385521..386684 (+)	1164	WP_404344648.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LG286_RS01835 (LG286_01830)	-	386697..387839 (-)	1143	WP_404344649.1	anhydro-N-acetylmuramic acid kinase	-
LG286_RS01840 (LG286_01835)	tyrS	387956..389155 (+)	1200	WP_404344650.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 42094.11 Da
• Isoelectric Point: 6.8279

>NTDB_id=616713 LG286_RS01830 WP_404344648.1 385521..386684(+) (pilU) [Vreelandella venusta strain ABH-461]
MSEPASGMTATQWLHQLLGIMVEQQASDLLISVGAPPSLKTPKGLMPLGRQPLSSSQVRSLVLNAVPEKQRERFQEEHEA
NFALSLENAGRFRVSAFQQRNEPAMVVRRIAQTIPTLSALGVPSQLAALANAKRGLVLVVGGTGTGKSTTLAAMIQERNA
SVGGHIITIEDPIEYLHPHQRGIVNQREVGVDTESFEVALKNTLRQAPDVILIGEVRTRETMEHALTFAETGHLCLATLH
ANNANQALERILHFFPHERHEQVLMDLSLNLRAVVAQQLLPTQQGGRCAAIEILLSSPRVVDLIRKGRIDELKQAMAGSR
DAGMQTFDQALYALYQAGQVSQQVALAHADSANDLRLMIKQGDAAVSGKPLDAQVPSHLALRDGDDY

Nucleotide

• Length: 1164 bp

>NTDB_id=616713 LG286_RS01830 WP_404344648.1 385521..386684(+) (pilU) [Vreelandella venusta strain ABH-461]
ATGAGTGAGCCAGCCAGCGGTATGACCGCGACCCAGTGGCTGCATCAGCTGCTGGGTATTATGGTCGAGCAGCAGGCCTC
CGACCTGCTGATTTCGGTGGGCGCGCCGCCCAGCTTGAAAACACCCAAGGGGCTGATGCCGCTAGGGCGTCAGCCGCTCT
CCTCTAGCCAGGTGCGCAGCCTGGTGCTGAATGCGGTTCCCGAGAAGCAGCGGGAGCGATTTCAAGAGGAGCACGAGGCG
AACTTTGCCCTAAGCCTGGAGAACGCGGGGCGGTTTCGGGTAAGCGCCTTCCAACAGCGCAACGAGCCCGCCATGGTGGT
GCGCCGTATTGCGCAGACTATTCCGACCCTCAGCGCGCTGGGCGTGCCTAGCCAGTTGGCGGCGTTGGCCAATGCCAAAC
GCGGGCTGGTGCTGGTGGTGGGCGGCACGGGAACGGGAAAATCCACTACCCTGGCGGCAATGATTCAGGAGCGTAACGCC
TCAGTCGGTGGGCACATCATCACCATTGAGGATCCTATCGAGTACTTGCATCCGCATCAGCGAGGCATCGTTAATCAGCG
CGAAGTGGGGGTGGATACGGAATCCTTCGAGGTGGCGCTGAAAAATACGCTACGCCAAGCGCCGGATGTGATCTTGATTG
GTGAAGTGCGTACCCGCGAGACCATGGAGCACGCCCTCACTTTCGCGGAAACAGGGCATCTCTGTTTGGCGACGCTGCAC
GCCAACAACGCTAACCAGGCGTTGGAGCGCATCCTTCACTTCTTTCCCCATGAGAGGCACGAGCAGGTGCTGATGGATCT
GTCGCTGAATCTGCGTGCCGTGGTCGCGCAGCAGCTTCTGCCTACTCAGCAGGGTGGCCGCTGCGCGGCGATTGAGATTC
TGCTCTCGTCGCCACGGGTAGTGGATCTGATCCGAAAAGGGCGTATTGATGAATTAAAGCAGGCCATGGCAGGATCCCGC
GACGCGGGGATGCAAACTTTCGATCAGGCGCTTTATGCGCTTTATCAAGCGGGCCAAGTCAGCCAGCAAGTGGCGCTGGC
CCATGCGGATTCCGCCAACGACCTGCGGCTGATGATCAAGCAGGGCGACGCCGCCGTCAGCGGTAAGCCGTTAGACGCGC
AGGTGCCTTCTCATCTTGCTCTACGCGACGGTGACGATTATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (39-281)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.695	96.641	0.587
pilU	Vibrio cholerae strain A1552	58.659	92.506	0.543
pilU	Acinetobacter baylyi ADP1	55.956	93.282	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.892	95.607	0.401
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	43.293	84.755	0.367
pilT	Vibrio cholerae strain A1552	43.293	84.755	0.367