Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG323_RS10145 Genome accession   NZ_CP085242
Coordinates   2165510..2166544 (+) Length   344 a.a.
NCBI ID   WP_310695354.1    Uniprot ID   -
Organism   Marinospirillum sp. strain ABH-597     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2160510..2171544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG323_RS10120 (LG323_10130) - 2160968..2161804 (-) 837 WP_404415637.1 peptidoglycan DD-metalloendopeptidase family protein -
  LG323_RS10125 (LG323_10135) xseA 2161842..2163218 (-) 1377 WP_404415640.1 exodeoxyribonuclease VII large subunit -
  LG323_RS10130 (LG323_10140) - 2163225..2163791 (-) 567 WP_404415642.1 YggT family protein -
  LG323_RS10135 (LG323_10145) proC 2163883..2164722 (-) 840 WP_310695356.1 pyrroline-5-carboxylate reductase -
  LG323_RS10140 (LG323_10150) - 2164751..2165446 (-) 696 WP_404415645.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG323_RS10145 (LG323_10155) pilT 2165510..2166544 (+) 1035 WP_310695354.1 type IV pilus twitching motility protein PilT Machinery gene
  LG323_RS10150 (LG323_10160) pilU 2166548..2167675 (+) 1128 WP_404415648.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG323_RS10155 (LG323_10165) - 2167656..2168903 (-) 1248 WP_404415651.1 heme biosynthesis protein HemY -
  LG323_RS10160 (LG323_10170) - 2168900..2170096 (-) 1197 WP_404415654.1 uroporphyrinogen-III C-methyltransferase -
  LG323_RS10165 (LG323_10175) - 2170112..2170873 (-) 762 WP_404415657.1 uroporphyrinogen-III synthase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38348.28 Da        Isoelectric Point: 6.4347

>NTDB_id=616307 LG323_RS10145 WP_310695354.1 2165510..2166544(+) (pilT) [Marinospirillum sp. strain ABH-597]
MDITELLAFCAKQNASDLHLSADLPPMIRVDGDIRRVNVPPMDHKEVHALVYDIMNDKQRRDYEEFMEVDFSFELPGLAR
FRVNAFNHNRGAGAVFRLIPSKVLTLDELGMGDIFRNLSMLPRGLVLITGPTGSGKSTTLAAMIDFVNERKNAHILTIED
PIEFVHESKKCLINQREIHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKSMVRSMLSESLQGVISQMLLKKEGGGRVAAHEIMIGTAAIRNLIREDKIAQMYSAIQTGATHGMQTMDQSL
KQLLTKGLISKEVAREKAKQPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=616307 LG323_RS10145 WP_310695354.1 2165510..2166544(+) (pilT) [Marinospirillum sp. strain ABH-597]
ATGGATATTACCGAATTGCTCGCCTTCTGCGCAAAGCAGAACGCCTCCGACCTGCATCTTTCTGCAGATCTGCCCCCGAT
GATCCGGGTTGATGGCGATATTCGCCGCGTCAATGTGCCGCCCATGGATCACAAGGAAGTTCACGCGCTGGTTTATGACA
TCATGAATGACAAGCAGCGCCGTGACTACGAAGAGTTCATGGAAGTGGATTTTTCCTTTGAGCTTCCGGGTCTGGCCCGC
TTCCGGGTCAATGCCTTCAATCACAACCGCGGTGCCGGTGCCGTTTTTCGCCTGATTCCTTCCAAGGTTTTGACTTTGGA
CGAGCTGGGCATGGGCGATATTTTTCGCAACCTCTCCATGCTGCCCCGTGGCCTGGTACTGATCACAGGGCCCACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCAATGATTGATTTTGTGAACGAGCGCAAAAATGCCCACATCCTCACCATTGAAGAC
CCCATTGAATTTGTCCACGAATCCAAGAAATGCCTGATCAACCAGCGTGAGATTCACCGTGACACCCTGGGCTTTTCCGA
AGCCTTGCGCTCGGCCCTGCGCGAAGACCCGGACATCATTCTGGTCGGTGAGATGCGGGATCTGGAAACCATCCGTCTGG
CACTGACAGCCGCAGAAACCGGCCACCTGGTATTTGGCACCCTGCACACCACCAGTGCCGCCAAAACCATTGACCGGATC
GTGGACGTCTTCCCGGCAGAGGAAAAATCCATGGTGCGCTCCATGCTTTCTGAATCCCTGCAGGGGGTAATTTCCCAGAT
GCTGCTCAAGAAGGAGGGCGGGGGTCGTGTTGCCGCCCATGAAATCATGATCGGCACTGCAGCTATTCGTAACCTGATTC
GTGAAGACAAGATTGCCCAGATGTACTCAGCCATCCAGACAGGGGCCACTCACGGCATGCAGACCATGGATCAGTCTCTG
AAACAGCTGCTGACCAAGGGTTTGATCAGCAAAGAAGTTGCGCGTGAAAAAGCCAAACAACCGGAAAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

83.14

100

0.831
  pilT Pseudomonas stutzeri DSM 10701

83.14

100

0.831
  pilT Acinetobacter baylyi ADP1

80.814

100

0.808
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Legionella pneumophila strain Lp02

71.302

98.256

0.701
  pilT Legionella pneumophila strain ERS1305867

71.302

98.256

0.701
  pilT Neisseria meningitidis 8013

68.696

100

0.689
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.656

97.965

0.663
  pilT Vibrio cholerae strain A1552

67.656

97.965

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39
  pilU Vibrio cholerae strain A1552

39.233

98.547

0.387