Detailed information NTG0616308

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LG323_RS10150
• Genome accession: NZ_CP085242
• Coordinates: 2166548..2167675 (+)
• Length: 375 a.a.
• NCBI ID: WP_404415648.1
• Uniprot ID: -
• Organism: Marinospirillum sp. strain ABH-597
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2161548..2172675

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LG323_RS10125 (LG323_10135)	xseA	2161842..2163218 (-)	1377	WP_404415640.1	exodeoxyribonuclease VII large subunit	-
LG323_RS10130 (LG323_10140)	-	2163225..2163791 (-)	567	WP_404415642.1	YggT family protein	-
LG323_RS10135 (LG323_10145)	proC	2163883..2164722 (-)	840	WP_310695356.1	pyrroline-5-carboxylate reductase	-
LG323_RS10140 (LG323_10150)	-	2164751..2165446 (-)	696	WP_404415645.1	YggS family pyridoxal phosphate-dependent enzyme	-
LG323_RS10145 (LG323_10155)	pilT	2165510..2166544 (+)	1035	WP_310695354.1	type IV pilus twitching motility protein PilT	Machinery gene
LG323_RS10150 (LG323_10160)	pilU	2166548..2167675 (+)	1128	WP_404415648.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LG323_RS10155 (LG323_10165)	-	2167656..2168903 (-)	1248	WP_404415651.1	heme biosynthesis protein HemY	-
LG323_RS10160 (LG323_10170)	-	2168900..2170096 (-)	1197	WP_404415654.1	uroporphyrinogen-III C-methyltransferase	-
LG323_RS10165 (LG323_10175)	-	2170112..2170873 (-)	762	WP_404415657.1	uroporphyrinogen-III synthase	-
LG323_RS10170 (LG323_10180)	hemC	2170873..2171805 (-)	933	WP_404415660.1	hydroxymethylbilane synthase	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 42011.04 Da
• Isoelectric Point: 6.1178

>NTDB_id=616308 LG323_RS10150 WP_404415648.1 2166548..2167675(+) (pilU) [Marinospirillum sp. strain ABH-597]
MKFTDFLKQMAERKGSDLFITTGMMPSMKVDGQLTPLTEQKLSPDQARELVFGVMNQEQKDEFRSNKECNFAISAPGIGR
FRVSAFYQRNQVGMVLRRIEFDIPQLDTLRLPEIIKDLAMTKRGLILFVGATGTGKSTSLASMIQYRNENARGHIISIED
PIEFIHPHKQSIVTQREVGIDTESFEVALKNTLRQAPDVIMIGEVRNRQTMEYALAFAETGHLCLATLHANNADQALERV
INFFPQESHNQVFMDLSLNLRGIVAQQLLPTVDGKGRRAAIEVLLNTPLVKDMVRKGDVHQLKSLMKKSRESGMQTFDQA
LFDLYNQGEISEEDALQHADSPNDLRLMIKLGSAEAGSSSAAFDKAASKFSLEDD

Nucleotide

• Length: 1128 bp

>NTDB_id=616308 LG323_RS10150 WP_404415648.1 2166548..2167675(+) (pilU) [Marinospirillum sp. strain ABH-597]
ATGAAATTTACTGACTTCCTCAAACAGATGGCCGAACGCAAGGGTTCGGACTTGTTCATCACCACCGGCATGATGCCTTC
CATGAAGGTGGACGGACAGCTGACACCTCTCACCGAGCAAAAACTCTCACCCGACCAGGCTCGGGAACTGGTGTTTGGCG
TCATGAACCAGGAGCAGAAGGATGAATTCCGTAGCAACAAGGAGTGCAACTTTGCCATCAGCGCACCCGGCATAGGCCGT
TTCCGGGTCAGTGCTTTTTATCAGCGCAACCAGGTGGGCATGGTACTGCGCCGTATCGAGTTTGATATTCCCCAACTGGA
CACTCTGCGCCTGCCGGAAATCATCAAGGATCTGGCCATGACCAAGCGTGGCCTCATCCTGTTTGTGGGGGCCACGGGAA
CCGGTAAATCCACCTCCCTGGCATCCATGATTCAGTACCGCAATGAAAACGCCCGGGGTCACATCATCAGCATTGAAGAC
CCTATTGAATTTATTCACCCCCACAAGCAAAGCATCGTCACCCAGCGTGAAGTGGGTATTGATACCGAAAGCTTTGAGGT
GGCCCTGAAAAACACCCTGCGCCAAGCACCGGATGTGATCATGATCGGGGAGGTGCGTAACCGCCAGACCATGGAATACG
CCCTGGCTTTTGCGGAAACCGGCCACCTTTGTTTGGCCACACTGCATGCCAATAACGCAGACCAGGCCCTGGAGCGGGTG
ATTAATTTTTTCCCCCAGGAAAGCCACAACCAGGTATTCATGGATCTCTCCCTGAATCTGCGTGGCATCGTTGCCCAGCA
ACTCTTGCCCACAGTGGATGGCAAGGGGCGCAGAGCAGCCATTGAGGTTCTGCTGAATACGCCACTGGTCAAGGATATGG
TTCGCAAGGGGGATGTGCATCAACTCAAATCCCTGATGAAGAAATCCCGGGAATCCGGCATGCAGACTTTTGATCAGGCT
TTGTTTGATCTCTACAACCAGGGTGAAATCAGTGAGGAGGATGCTCTGCAGCATGCCGACTCACCCAATGACCTGCGCCT
GATGATCAAGCTGGGCAGTGCCGAAGCGGGCAGTAGCTCGGCAGCCTTTGACAAGGCCGCCAGTAAGTTCAGCCTGGAGG
ATGACTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	68.8	100	0.688
pilU	Acinetobacter baylyi ADP1	62.849	95.467	0.6
pilU	Vibrio cholerae strain A1552	56	93.333	0.523
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.278	96	0.387