Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG301_RS14420 Genome accession   NZ_CP085241
Coordinates   3100069..3101112 (-) Length   347 a.a.
NCBI ID   WP_404471846.1    Uniprot ID   -
Organism   Vreelandella venusta strain ABH-602     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3095069..3106112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG301_RS14405 (LG301_14390) tyrS 3096431..3097630 (-) 1200 WP_404471844.1 tyrosine--tRNA ligase -
  LG301_RS14410 (LG301_14395) - 3097747..3098889 (+) 1143 WP_404475280.1 anhydro-N-acetylmuramic acid kinase -
  LG301_RS14415 (LG301_14400) pilU 3098902..3100065 (-) 1164 WP_404471845.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG301_RS14420 (LG301_14405) pilT 3100069..3101112 (-) 1044 WP_404471846.1 type IV pilus twitching motility protein PilT Machinery gene
  LG301_RS14425 (LG301_14410) - 3101146..3101841 (+) 696 WP_404471847.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG301_RS14430 (LG301_14415) proC 3101928..3102752 (+) 825 WP_404471848.1 pyrroline-5-carboxylate reductase -
  LG301_RS14435 (LG301_14420) - 3102782..3103375 (+) 594 WP_404471849.1 YggT family protein -
  LG301_RS14440 (LG301_14425) - 3103457..3104665 (+) 1209 WP_404471850.1 homoserine O-acetyltransferase -
  LG301_RS14445 (LG301_14430) metW 3104665..3105258 (+) 594 WP_263067274.1 methionine biosynthesis protein MetW -
  LG301_RS14450 (LG301_14435) - 3105290..3105739 (+) 450 WP_404471851.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38152.58 Da        Isoelectric Point: 6.0549

>NTDB_id=616292 LG301_RS14420 WP_404471846.1 3100069..3101112(-) (pilT) [Vreelandella venusta strain ABH-602]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMDNNEVRRLIYDIMNDQQRRDYEEHLETDFSFDVPGVAR
FRVNAFNQARGVGAVFRTIPNDVLSMQALGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRFEHILTIED
PVEFVHASKRCLINQREVHRDTRSFNSALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKSMVRSMLSESLQAVVSQALLKRQGGGRVAAHEILIATAAVRNLIREDKVAQIYSAIQTGGNLGMQTLNASL
AKLVKEGVVSMEDAQARAKGTLSLGDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=616292 LG301_RS14420 WP_404471846.1 3100069..3101112(-) (pilT) [Vreelandella venusta strain ABH-602]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAATGCATCCGACCTGCACCTTTCGGCGGGTCTCCCCCCCAT
GATACGTGTTGATGGCGATATTCGTCGGCTTAATGTGCCGGCCATGGATAACAACGAAGTCAGGCGGTTGATCTACGACA
TCATGAACGATCAGCAGCGCCGCGACTATGAGGAGCACTTGGAAACCGATTTCTCATTTGACGTGCCGGGGGTGGCGCGT
TTTAGGGTCAATGCGTTTAACCAAGCGCGAGGCGTGGGAGCCGTTTTTCGTACTATTCCAAATGACGTGCTTTCTATGCA
GGCGCTGGGCCTTGGTGAGGTGTTCGAGCGTTTGGCGATGCTGCCCCGTGGACTAGTGCTTGTGACGGGGCCAACAGGGT
CAGGTAAAAGCACGACACTCGCCGCGATGATTGATTACATTAATGACCATCGCTTTGAGCACATTCTGACCATCGAAGAT
CCGGTGGAATTTGTGCATGCCAGTAAGCGCTGTTTAATCAATCAACGCGAAGTGCATCGCGATACGCGCAGCTTCAACAG
CGCATTACGCAGTGCCTTACGTGAAGACCCGGACGTTATCTTAGTGGGCGAGCTGCGCGATTTGGAAACGATTCGCCTAG
CGCTGACCGCGGCAGAAACGGGGCACCTAGTGTTTGGCACGCTGCATACAACCTCCGCTGCCAAAACCATCGATCGGATT
ATTGACGTGTTTCCCGGTGAGGAGAAATCCATGGTGCGCTCGATGCTGTCGGAGTCGCTTCAAGCGGTTGTGTCGCAAGC
GCTATTAAAGCGCCAAGGGGGTGGGCGCGTCGCGGCTCACGAGATATTAATTGCGACCGCTGCCGTTCGAAATCTCATCC
GTGAGGATAAAGTGGCGCAGATTTACTCGGCTATTCAAACCGGGGGTAATCTGGGCATGCAAACCTTAAACGCCTCGCTG
GCTAAACTGGTCAAAGAGGGGGTGGTGAGCATGGAGGATGCTCAAGCACGTGCAAAAGGGACGCTTTCGTTAGGGGACGA
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.581

97.695

0.758
  pilT Pseudomonas aeruginosa PAK

77.286

97.695

0.755
  pilT Acinetobacter nosocomialis M2

76.401

97.695

0.746
  pilT Acinetobacter baumannii D1279779

76.401

97.695

0.746
  pilT Acinetobacter baumannii strain A118

76.401

97.695

0.746
  pilT Acinetobacter baylyi ADP1

74.709

99.135

0.741
  pilT Legionella pneumophila strain Lp02

71.006

97.406

0.692
  pilT Legionella pneumophila strain ERS1305867

71.006

97.406

0.692
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.118

0.674
  pilT Vibrio cholerae strain A1552

69.436

97.118

0.674
  pilT Neisseria meningitidis 8013

66.377

99.424

0.66
  pilT Neisseria gonorrhoeae MS11

66.087

99.424

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.687

97.695

0.524
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

40.882

97.983

0.401
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392