Detailed information NTG0616291

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LG301_RS14415
• Genome accession: NZ_CP085241
• Coordinates: 3098902..3100065 (-)
• Length: 387 a.a.
• NCBI ID: WP_404471845.1
• Uniprot ID: -
• Organism: Vreelandella venusta strain ABH-602
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3093902..3105065

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LG301_RS14405 (LG301_14390)	tyrS	3096431..3097630 (-)	1200	WP_404471844.1	tyrosine--tRNA ligase	-
LG301_RS14410 (LG301_14395)	-	3097747..3098889 (+)	1143	WP_404475280.1	anhydro-N-acetylmuramic acid kinase	-
LG301_RS14415 (LG301_14400)	pilU	3098902..3100065 (-)	1164	WP_404471845.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LG301_RS14420 (LG301_14405)	pilT	3100069..3101112 (-)	1044	WP_404471846.1	type IV pilus twitching motility protein PilT	Machinery gene
LG301_RS14425 (LG301_14410)	-	3101146..3101841 (+)	696	WP_404471847.1	YggS family pyridoxal phosphate-dependent enzyme	-
LG301_RS14430 (LG301_14415)	proC	3101928..3102752 (+)	825	WP_404471848.1	pyrroline-5-carboxylate reductase	-
LG301_RS14435 (LG301_14420)	-	3102782..3103375 (+)	594	WP_404471849.1	YggT family protein	-
LG301_RS14440 (LG301_14425)	-	3103457..3104665 (+)	1209	WP_404471850.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 42257.26 Da
• Isoelectric Point: 6.6200

>NTDB_id=616291 LG301_RS14415 WP_404471845.1 3098902..3100065(-) (pilU) [Vreelandella venusta strain ABH-602]
MSEPTNGMTASQWLHQLLAIMIEQQASDLLISVGAPPSLKTSQGLVPLGRQPLSPSQVRSLVISAIPDMQRERFQLEHEA
NFALSLEQKGRFRVSAFQQRNEPAMVVRRIAQDIPTLSALGVPSQLAELANAKRGLVLVVGGTGTGKSTTLAAMIQERNA
SVGGHIITIEDPIEYLHPHQRGIVNQREVGVDTESFEVALKNTLRQAPDVILIGEVRTRETMEHALTFAETGHLCLATLH
ANNANQALERILHFFPHERHEQIRMDLSLNLRAVVAQQLLPTLQGGRCAAIEIMLSSPRVVDLIRKGRIDELKSAMAGSR
DAGMQTFDQALYALYQAEQISQQVALAHADSANDLRLMIKHGDAAASGTSLASQVSPHLALRDGDDY

Nucleotide

• Length: 1164 bp

>NTDB_id=616291 LG301_RS14415 WP_404471845.1 3098902..3100065(-) (pilU) [Vreelandella venusta strain ABH-602]
ATGAGTGAGCCAACAAACGGTATGACAGCCTCCCAGTGGTTGCACCAACTGCTGGCCATTATGATTGAGCAGCAGGCGTC
GGACCTGCTTATTTCGGTGGGGGCGCCGCCAAGTTTGAAAACCTCCCAGGGGCTGGTGCCGTTGGGGCGGCAACCGCTTT
CGCCCAGCCAAGTTCGCAGTCTGGTGATAAGCGCAATTCCTGACATGCAGCGGGAGCGGTTTCAGCTAGAGCATGAAGCA
AACTTTGCGCTTAGTCTTGAGCAGAAAGGCCGTTTTCGGGTGAGTGCCTTTCAGCAGCGTAATGAACCGGCCATGGTGGT
GCGTCGTATTGCTCAAGATATCCCTACGCTCAGCGCCTTGGGCGTGCCCAGCCAGCTGGCCGAACTCGCCAACGCCAAGC
GTGGGTTGGTGTTGGTGGTCGGGGGTACCGGAACCGGTAAGTCCACTACCCTTGCTGCGATGATCCAAGAGCGTAATGCC
TCCGTGGGTGGACACATCATCACCATCGAAGATCCCATCGAGTATTTGCACCCTCACCAGCGCGGCATTGTTAATCAGCG
TGAAGTGGGGGTGGATACAGAGTCCTTCGAAGTGGCGCTGAAAAACACGCTTCGCCAAGCGCCGGATGTGATCCTGATTG
GCGAAGTGCGTACCCGGGAAACCATGGAGCATGCCCTGACGTTTGCGGAAACCGGCCATCTCTGTTTGGCGACGCTGCAT
GCCAACAATGCGAACCAAGCGCTGGAGCGTATCCTCCACTTTTTCCCCCATGAGCGGCATGAACAAATACGCATGGACTT
ATCGCTCAACCTTCGTGCGGTGGTCGCGCAGCAGCTGTTGCCCACTCTGCAGGGCGGCCGCTGTGCAGCGATTGAAATTA
TGCTCTCCTCCCCTCGAGTGGTGGATCTAATACGAAAAGGCCGTATCGATGAGCTGAAGTCAGCTATGGCGGGCTCTCGT
GATGCGGGTATGCAAACGTTTGATCAAGCGCTTTACGCGCTTTACCAAGCGGAGCAAATCAGTCAGCAGGTCGCGCTGGC
GCATGCGGACTCTGCCAATGATCTTCGGCTGATGATTAAGCATGGTGATGCGGCGGCCAGCGGTACATCGCTGGCCTCGC
AGGTATCCCCCCATTTGGCGTTGCGCGACGGCGACGATTATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (22-281)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.16	96.641	0.581
pilU	Vibrio cholerae strain A1552	58.496	92.765	0.543
pilU	Acinetobacter baylyi ADP1	56.215	91.473	0.514
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.857	95.866	0.411
pilT	Legionella pneumophila strain Lp02	43.03	85.271	0.367
pilT	Legionella pneumophila strain ERS1305867	43.03	85.271	0.367
pilT	Neisseria meningitidis 8013	40.936	88.372	0.362