Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LG272_RS05560 Genome accession   NZ_CP085233
Coordinates   1173374..1174489 (+) Length   371 a.a.
NCBI ID   WP_404409944.1    Uniprot ID   -
Organism   Pseudidiomarina marina strain ADH-25     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1168374..1179489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG272_RS05535 (LG272_05530) - 1169757..1170194 (-) 438 WP_404409941.1 DUF4426 domain-containing protein -
  LG272_RS05540 (LG272_05535) - 1170191..1170742 (-) 552 WP_404409942.1 YggT family protein -
  LG272_RS05545 (LG272_05540) proC 1170765..1171592 (-) 828 WP_126758386.1 pyrroline-5-carboxylate reductase -
  LG272_RS05550 (LG272_05545) - 1171589..1172302 (-) 714 WP_404409943.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG272_RS05555 (LG272_05550) pilT 1172337..1173371 (+) 1035 WP_126758388.1 type IV pilus twitching motility protein PilT Machinery gene
  LG272_RS05560 (LG272_05555) pilU 1173374..1174489 (+) 1116 WP_404409944.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG272_RS05565 (LG272_05560) tsaD 1174552..1175580 (+) 1029 WP_126758390.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  LG272_RS05570 (LG272_05565) plsY 1175589..1176179 (-) 591 WP_404409945.1 glycerol-3-phosphate 1-O-acyltransferase PlsY -
  LG272_RS05575 (LG272_05570) folB 1176329..1176679 (+) 351 WP_404409946.1 dihydroneopterin aldolase -
  LG272_RS05580 (LG272_05575) folK 1176679..1177209 (+) 531 WP_404409947.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase -
  LG272_RS05585 (LG272_05580) - 1177225..1178019 (+) 795 WP_404409948.1 undecaprenyl-diphosphate phosphatase -
  LG272_RS05590 - 1178012..1179127 (-) 1116 WP_404409949.1 CCA tRNA nucleotidyltransferase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40898.13 Da        Isoelectric Point: 5.8688

>NTDB_id=616174 LG272_RS05560 WP_404409944.1 1173374..1174489(+) (pilU) [Pseudidiomarina marina strain ADH-25]
MVLDKLLKIMVERNSSDLFISAGLPPSAKINGELTPLTEQRLSDNAALAIVKEALGVKHLNEFMDEKEANFAIFREGIGR
FRVSAFWQRQRAGMVVRRIVTEIPTVEELKLPEVLTKLIMNKRGLMLVVGATGSGKSTTLAALIGYRNQHSKGHILTIED
PVEFVHEHAKSMITQREVGSDTESFEAALKSSLRQAPDVILVGEIRSQETMEYALTFAETGHLCVATLHANNANQAIERI
MHMVPKEKIGKLMFDLSLNLRGIVAQQLIPTIDGGRVAAIEILLNSPIVAEKIATNDMGEIKEIMAKSRELGMQTFDQAL
FDLYKAGRISYDEALRHADAPNDLRLRIKLSGDSNASDDDSALQGATVDLD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=616174 LG272_RS05560 WP_404409944.1 1173374..1174489(+) (pilU) [Pseudidiomarina marina strain ADH-25]
ATGGTTCTAGATAAACTTTTAAAAATAATGGTGGAACGCAATAGCTCGGATTTATTTATCTCCGCGGGCTTACCGCCAAG
TGCAAAAATTAATGGTGAATTGACACCGTTGACCGAACAAAGACTCAGTGACAATGCGGCGCTAGCTATTGTCAAAGAAG
CGTTGGGTGTTAAACATCTGAATGAGTTCATGGATGAAAAGGAAGCCAACTTTGCGATATTTCGTGAAGGTATCGGCCGC
TTTCGGGTGAGTGCATTTTGGCAACGCCAACGTGCGGGAATGGTGGTCCGTCGTATTGTTACCGAAATTCCAACAGTGGA
AGAGCTGAAGCTACCAGAAGTACTGACTAAGCTAATTATGAATAAACGTGGATTAATGCTGGTAGTCGGTGCCACAGGCT
CCGGTAAATCGACCACGTTGGCAGCATTGATCGGTTATCGAAACCAGCATTCGAAAGGTCACATTTTAACAATTGAAGAT
CCCGTCGAATTTGTTCATGAACACGCGAAGTCAATGATCACGCAACGGGAAGTCGGCAGTGATACGGAATCGTTTGAAGC
CGCGTTGAAAAGCTCATTGCGTCAAGCGCCTGACGTCATCTTGGTGGGTGAAATTCGCTCGCAAGAAACTATGGAGTACG
CACTTACCTTCGCGGAAACCGGTCATTTATGTGTGGCCACACTGCACGCTAACAACGCCAACCAAGCGATTGAACGCATC
ATGCACATGGTACCTAAGGAAAAAATCGGCAAGTTGATGTTTGACTTGTCTTTGAACCTCCGCGGTATCGTCGCGCAACA
GTTAATTCCAACCATTGACGGTGGGCGTGTCGCAGCAATTGAAATCTTATTAAATAGCCCGATTGTTGCGGAAAAAATTG
CAACCAATGATATGGGTGAAATTAAAGAGATAATGGCGAAATCGCGCGAGCTTGGTATGCAAACCTTTGACCAAGCACTA
TTCGACCTCTACAAAGCTGGTCGTATTAGTTATGACGAAGCCTTGCGTCATGCTGACGCGCCAAACGATTTGCGCTTGCG
TATTAAACTGAGTGGCGATAGTAATGCAAGTGATGATGACAGCGCTTTGCAGGGAGCAACGGTTGATCTCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

56.568

100

0.569
  pilU Pseudomonas stutzeri DSM 10701

56.604

100

0.566
  pilU Acinetobacter baylyi ADP1

56.906

97.574

0.555
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.477

94.879

0.394
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.643

89.757

0.383
  pilT Vibrio cholerae strain A1552

42.643

89.757

0.383
  pilT Pseudomonas aeruginosa PAK

40.988

92.722

0.38
  pilT Legionella pneumophila strain Lp02

41.617

90.027

0.375
  pilT Legionella pneumophila strain ERS1305867

41.617

90.027

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.116

92.722

0.372
  pilT Neisseria meningitidis 8013

39.528

91.375

0.361
  pilT Neisseria gonorrhoeae MS11

39.528

91.375

0.361
  pilT Acinetobacter baylyi ADP1

39.296

91.914

0.361
  pilT Acinetobacter baumannii strain A118

39.296

91.914

0.361
  pilT Acinetobacter nosocomialis M2

39.296

91.914

0.361
  pilT Acinetobacter baumannii D1279779

39.296

91.914

0.361