Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG272_RS05555 Genome accession   NZ_CP085233
Coordinates   1172337..1173371 (+) Length   344 a.a.
NCBI ID   WP_126758388.1    Uniprot ID   -
Organism   Pseudidiomarina marina strain ADH-25     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1167337..1178371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG272_RS05530 (LG272_05525) - 1167987..1169726 (+) 1740 WP_276567241.1 potassium/proton antiporter -
  LG272_RS05535 (LG272_05530) - 1169757..1170194 (-) 438 WP_404409941.1 DUF4426 domain-containing protein -
  LG272_RS05540 (LG272_05535) - 1170191..1170742 (-) 552 WP_404409942.1 YggT family protein -
  LG272_RS05545 (LG272_05540) proC 1170765..1171592 (-) 828 WP_126758386.1 pyrroline-5-carboxylate reductase -
  LG272_RS05550 (LG272_05545) - 1171589..1172302 (-) 714 WP_404409943.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG272_RS05555 (LG272_05550) pilT 1172337..1173371 (+) 1035 WP_126758388.1 type IV pilus twitching motility protein PilT Machinery gene
  LG272_RS05560 (LG272_05555) pilU 1173374..1174489 (+) 1116 WP_404409944.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG272_RS05565 (LG272_05560) tsaD 1174552..1175580 (+) 1029 WP_126758390.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  LG272_RS05570 (LG272_05565) plsY 1175589..1176179 (-) 591 WP_404409945.1 glycerol-3-phosphate 1-O-acyltransferase PlsY -
  LG272_RS05575 (LG272_05570) folB 1176329..1176679 (+) 351 WP_404409946.1 dihydroneopterin aldolase -
  LG272_RS05580 (LG272_05575) folK 1176679..1177209 (+) 531 WP_404409947.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase -
  LG272_RS05585 (LG272_05580) - 1177225..1178019 (+) 795 WP_404409948.1 undecaprenyl-diphosphate phosphatase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38250.74 Da        Isoelectric Point: 6.9354

>NTDB_id=616173 LG272_RS05555 WP_126758388.1 1172337..1173371(+) (pilT) [Pseudidiomarina marina strain ADH-25]
MDITELLSFAVKHKSSDLHLSADSPPMIRVDGDVRKINIPALSHKQVQELVYDIMNDRQRKEYEQNLECDFSFEVPDLAR
FRVNAFVQNRGAGAVFRTIPSEVLTLEQLGAPEIFKTISDNPRGLVLVTGPTGSGKSTTLAAMIDYVNENKYHHILTIED
PIEFVHKNKRSLINQREVHRDTHSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGDEKPMIRSMLSESLRAVISQTLLKKVGGGRVAAHEIMIATSAIKNLIREDKIAQMYSSIQTGAAHGMQTLDQCL
QNLLNQGLVSRADAKAKAANKDSF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=616173 LG272_RS05555 WP_126758388.1 1172337..1173371(+) (pilT) [Pseudidiomarina marina strain ADH-25]
ATGGATATCACTGAGTTACTCAGTTTCGCTGTTAAACATAAATCGTCAGACTTACATTTATCAGCTGATTCGCCTCCTAT
GATACGGGTAGACGGTGACGTTCGTAAAATAAATATCCCAGCGCTAAGCCACAAGCAGGTGCAAGAGCTGGTATACGACA
TCATGAACGACCGCCAGCGTAAAGAATACGAACAAAACTTAGAATGCGACTTCTCGTTTGAAGTTCCTGATTTAGCCCGT
TTTCGTGTTAACGCATTCGTGCAGAATCGTGGTGCTGGCGCTGTATTTCGAACCATTCCAAGTGAGGTGCTCACCTTGGA
ACAGTTGGGTGCGCCAGAGATATTCAAGACTATTTCAGATAATCCGCGTGGTTTAGTGTTGGTTACAGGGCCAACAGGTT
CAGGTAAGTCGACAACATTAGCGGCCATGATCGATTACGTTAATGAAAATAAATATCATCATATTTTAACCATCGAAGAT
CCGATTGAATTTGTGCACAAAAACAAGCGTAGCCTGATTAACCAGCGGGAAGTGCACCGTGATACACACAGTTTTAATAA
CGCACTGCGCTCTGCATTACGTGAAGACCCTGATGTGATACTTGTGGGTGAGATGCGTGATCTTGAAACAATCCGCCTAG
CAATGACCGCCGCAGAAACCGGTCACTTGGTGTTTGGTACCCTACACACCACGTCAGCGCCGAAAACCATTGACCGTATT
GTTGATGTGTTTCCTGGCGACGAGAAACCGATGATTCGCTCAATGTTGTCCGAATCATTACGCGCAGTTATTTCGCAAAC
GCTACTGAAGAAAGTCGGTGGCGGTCGTGTTGCTGCGCACGAAATCATGATTGCGACTTCAGCCATTAAAAACCTCATTC
GTGAAGATAAAATTGCGCAAATGTATTCGTCGATTCAAACCGGTGCGGCGCATGGTATGCAAACATTAGACCAATGCTTG
CAGAATCTGTTGAACCAAGGCCTCGTTTCTCGTGCCGATGCGAAGGCCAAAGCCGCGAACAAGGACTCATTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.326

100

0.773
  pilT Pseudomonas aeruginosa PAK

76.163

100

0.762
  pilT Acinetobacter nosocomialis M2

75.872

100

0.759
  pilT Acinetobacter baumannii D1279779

75.872

100

0.759
  pilT Acinetobacter baumannii strain A118

75.872

100

0.759
  pilT Acinetobacter baylyi ADP1

75

100

0.75
  pilT Legionella pneumophila strain Lp02

72.965

100

0.73
  pilT Legionella pneumophila strain ERS1305867

72.965

100

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.513

97.965

0.701
  pilT Vibrio cholerae strain A1552

71.513

97.965

0.701
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

41.983

99.709

0.419
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

39.881

97.674

0.39