Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG300_RS14025 Genome accession   NZ_CP085232
Coordinates   3020573..3021616 (-) Length   347 a.a.
NCBI ID   WP_404375929.1    Uniprot ID   -
Organism   Vreelandella aquamarina strain ADH-27     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3015573..3026616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG300_RS14010 (LG300_14015) tyrS 3016914..3018113 (-) 1200 WP_404375926.1 tyrosine--tRNA ligase -
  LG300_RS14015 (LG300_14020) - 3018230..3019372 (+) 1143 WP_404375927.1 anhydro-N-acetylmuramic acid kinase -
  LG300_RS14020 (LG300_14025) pilU 3019385..3020548 (-) 1164 WP_404375928.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG300_RS14025 (LG300_14030) pilT 3020573..3021616 (-) 1044 WP_404375929.1 type IV pilus twitching motility protein PilT Machinery gene
  LG300_RS14030 (LG300_14035) - 3021650..3022348 (+) 699 WP_404375930.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG300_RS14035 (LG300_14040) proC 3022437..3023267 (+) 831 WP_404375931.1 pyrroline-5-carboxylate reductase -
  LG300_RS14040 (LG300_14045) - 3023400..3023993 (+) 594 WP_404375932.1 YggT family protein -
  LG300_RS14045 (LG300_14050) - 3024067..3025263 (+) 1197 WP_404375933.1 homoserine O-acetyltransferase -
  LG300_RS14050 (LG300_14055) metW 3025263..3025859 (+) 597 WP_404375935.1 methionine biosynthesis protein MetW -
  LG300_RS14055 (LG300_14060) - 3025896..3026357 (+) 462 WP_404375936.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38225.74 Da        Isoelectric Point: 6.5071

>NTDB_id=616159 LG300_RS14025 WP_404375929.1 3020573..3021616(-) (pilT) [Vreelandella aquamarina strain ADH-27]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMENNEVRRLIYDIMNDRQRRDYEEHLEADFSFEVPGVAR
FRVNAFNQARGAGAVFRTIPNKVLSMDTLGMGEVFQRLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRFEHILTIED
PVEFVHTSKRCLINQREVHRDTHSFSGALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKSMVRSMLSESLQAVISQTLLKRQGGGRVAAHEILIATAAVRNLIREDKVAQIYSAIQTGGALGMQTLNASL
AKLVKEGVVSMDDAQARAKGTLTLNDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=616159 LG300_RS14025 WP_404375929.1 3020573..3021616(-) (pilT) [Vreelandella aquamarina strain ADH-27]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAATGCATCCGACCTGCACCTTTCGGCGGGTCTTCCCCCCAT
GATACGTGTTGATGGCGATATTCGTCGGCTTAATGTGCCTGCCATGGAGAATAACGAGGTTCGGCGGCTGATCTACGACA
TCATGAACGATCGGCAGCGCCGCGACTACGAAGAGCACTTGGAAGCCGACTTCTCGTTTGAAGTGCCGGGGGTGGCGCGC
TTTAGGGTCAATGCCTTTAACCAGGCGCGCGGGGCGGGGGCAGTGTTCCGAACCATTCCGAATAAGGTGCTCTCCATGGA
TACCTTGGGAATGGGCGAGGTGTTCCAGCGCCTTTCGATGCTGCCAAGGGGGTTGGTGCTAGTGACCGGGCCGACGGGGT
CGGGGAAAAGCACGACGTTGGCGGCGATGATTGACTACATCAACGACCACCGCTTTGAGCATATCCTGACCATTGAAGAC
CCGGTGGAGTTTGTACACACCAGCAAACGCTGCTTGATCAACCAGCGGGAGGTCCACCGCGATACCCATAGTTTCTCGGG
GGCACTGAGAAGCGCGCTGCGCGAAGACCCAGACGTGATTTTGGTGGGGGAGCTTCGCGATCTCGAAACGATTCGCCTGG
CGCTCACCGCGGCTGAAACGGGGCATTTGGTATTCGGCACCCTGCACACCACTTCCGCAGCGAAAACCATCGACCGGATT
ATTGATGTCTTCCCCGGCGAAGAGAAATCTATGGTGCGCTCGATGCTCTCTGAATCGCTTCAGGCGGTTATTTCGCAAAC
GCTGTTGAAGCGCCAGGGCGGCGGACGCGTGGCGGCTCACGAGATCCTGATCGCCACGGCAGCGGTACGTAACTTGATTC
GCGAGGATAAGGTGGCGCAGATCTACTCAGCCATTCAGACCGGCGGCGCGTTGGGGATGCAAACCCTCAATGCTTCGCTC
GCCAAACTGGTCAAAGAGGGGGTGGTGAGTATGGACGACGCCCAGGCGCGGGCCAAAGGCACGCTTACGCTAAACGATGA
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.351

97.695

0.775
  pilT Pseudomonas aeruginosa PAK

79.351

97.695

0.775
  pilT Acinetobacter nosocomialis M2

77.286

97.695

0.755
  pilT Acinetobacter baumannii D1279779

77.286

97.695

0.755
  pilT Acinetobacter baumannii strain A118

77.286

97.695

0.755
  pilT Acinetobacter baylyi ADP1

75.516

97.695

0.738
  pilT Legionella pneumophila strain Lp02

71.006

97.406

0.692
  pilT Legionella pneumophila strain ERS1305867

71.006

97.406

0.692
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

97.118

0.683
  pilT Vibrio cholerae strain A1552

70.326

97.118

0.683
  pilT Neisseria meningitidis 8013

66.474

99.712

0.663
  pilT Neisseria gonorrhoeae MS11

66.185

99.712

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.392

97.695

0.522
  pilU Acinetobacter baylyi ADP1

40.882

97.983

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395
  pilU Vibrio cholerae strain A1552

40.896

96.542

0.395