Detailed information NTG0616158

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LG300_RS14020
• Genome accession: NZ_CP085232
• Coordinates: 3019385..3020548 (-)
• Length: 387 a.a.
• NCBI ID: WP_404375928.1
• Uniprot ID: -
• Organism: Vreelandella aquamarina strain ADH-27
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3014385..3025548

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LG300_RS14010 (LG300_14015)	tyrS	3016914..3018113 (-)	1200	WP_404375926.1	tyrosine--tRNA ligase	-
LG300_RS14015 (LG300_14020)	-	3018230..3019372 (+)	1143	WP_404375927.1	anhydro-N-acetylmuramic acid kinase	-
LG300_RS14020 (LG300_14025)	pilU	3019385..3020548 (-)	1164	WP_404375928.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LG300_RS14025 (LG300_14030)	pilT	3020573..3021616 (-)	1044	WP_404375929.1	type IV pilus twitching motility protein PilT	Machinery gene
LG300_RS14030 (LG300_14035)	-	3021650..3022348 (+)	699	WP_404375930.1	YggS family pyridoxal phosphate-dependent enzyme	-
LG300_RS14035 (LG300_14040)	proC	3022437..3023267 (+)	831	WP_404375931.1	pyrroline-5-carboxylate reductase	-
LG300_RS14040 (LG300_14045)	-	3023400..3023993 (+)	594	WP_404375932.1	YggT family protein	-
LG300_RS14045 (LG300_14050)	-	3024067..3025263 (+)	1197	WP_404375933.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 42000.97 Da
• Isoelectric Point: 7.0151

>NTDB_id=616158 LG300_RS14020 WP_404375928.1 3019385..3020548(-) (pilU) [Vreelandella aquamarina strain ADH-27]
MSEPASGMTATQWLHQLLGIMVEQQASDLLISVGAPPSLKTPQGLVPLGRQPLSASQVRSLVLNAVPDKQRERFQAEHEA
NFALSLESAGRFRVSAFQQRNEPAMVVRRIAQQIPTLSALGVPSQLSTLANAKRGLVLVVGGTGTGKSTTLAAMIQERNA
SVGGHIITIEDPIEYLHPHQRGIVNQREVGVDTESFEVALKNTLRQAPDVILIGEVRTRETMEHALTFAETGHLCLATLH
ANNANQALERILHFFPHERHEQVLMDLSLNLRAVVAQQLLPTQQGGRCAAIEILLSSPRVVDLIRKGRIDELKQAMAGSR
DAGMQTFDQALYALYQAGKVSQQVALAHADSANDLRLMIKHGDAAASGKPLDAQVPSHLALRDGDDY

Nucleotide

• Length: 1164 bp

>NTDB_id=616158 LG300_RS14020 WP_404375928.1 3019385..3020548(-) (pilU) [Vreelandella aquamarina strain ADH-27]
ATGAGTGAGCCAGCCAGCGGTATGACCGCGACCCAGTGGCTGCATCAACTGCTGGGGATCATGGTCGAGCAGCAGGCCTC
TGACCTGCTTATTTCAGTGGGCGCGCCGCCCAGCCTTAAAACGCCCCAAGGGCTGGTGCCGCTGGGGCGTCAGCCGCTCT
CTGCTAGCCAGGTGCGTAGTTTGGTGCTGAATGCGGTTCCCGATAAGCAGCGGGAGCGATTTCAAGCTGAGCACGAGGCG
AATTTTGCCCTAAGCCTGGAAAGCGCGGGACGGTTTCGGGTGAGCGCCTTCCAACAGCGCAACGAACCCGCCATGGTGGT
GCGCCGCATTGCCCAGCAAATTCCCACGCTGAGCGCGCTGGGGGTGCCTAGCCAGTTGTCGACGCTGGCCAATGCCAAGC
GCGGGCTGGTGCTGGTCGTGGGTGGCACGGGAACGGGGAAATCCACCACGCTGGCGGCGATGATTCAGGAGCGTAACGCC
TCGGTGGGCGGGCATATCATCACCATTGAGGACCCCATCGAGTATTTACATCCGCACCAGCGGGGCATCGTTAATCAGCG
CGAAGTGGGGGTGGATACGGAGTCCTTCGAGGTGGCGCTGAAAAATACGCTACGCCAAGCGCCGGATGTGATCTTGATTG
GCGAAGTGCGTACCCGCGAGACCATGGAGCACGCCCTGACCTTCGCTGAAACGGGGCATCTTTGCCTGGCGACGCTGCAC
GCCAACAACGCTAACCAGGCGCTGGAACGTATCCTGCACTTCTTTCCCCACGAACGGCACGAGCAGGTGCTGATGGACCT
ATCGCTGAATCTGCGCGCCGTGGTGGCTCAGCAGCTGCTGCCTACCCAACAGGGTGGCCGCTGCGCGGCGATTGAGATTC
TGCTCTCATCGCCGCGGGTGGTGGATCTGATTCGTAAAGGGCGTATTGATGAATTGAAACAGGCCATGGCAGGCTCTCGC
GATGCGGGGATGCAAACCTTTGATCAGGCGCTTTACGCGCTCTATCAAGCGGGTAAAGTGAGCCAACAGGTAGCGCTGGC
CCATGCGGACTCCGCCAACGACCTGCGGCTGATGATCAAACACGGTGATGCCGCCGCCAGCGGTAAGCCGTTAGACGCAC
AGGTGCCGTCCCATCTGGCCCTGCGCGACGGTGACGATTACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-281)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.16	96.641	0.581
pilU	Vibrio cholerae strain A1552	59.497	92.506	0.55
pilU	Acinetobacter baylyi ADP1	55.679	93.282	0.519
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.351	95.607	0.395
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	43.293	84.755	0.367
pilT	Vibrio cholerae strain A1552	43.293	84.755	0.367