Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG331_RS01745 Genome accession   NZ_CP085222
Coordinates   356191..357234 (+) Length   347 a.a.
NCBI ID   WP_253019159.1    Uniprot ID   -
Organism   Vreelandella aquamarina strain ADH2-948     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 351191..362234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG331_RS01715 (LG331_01710) - 351466..351927 (-) 462 WP_404344642.1 DUF4426 domain-containing protein -
  LG331_RS01720 (LG331_01715) metW 351983..352579 (-) 597 WP_404344643.1 methionine biosynthesis protein MetW -
  LG331_RS01725 (LG331_01720) - 352579..353775 (-) 1197 WP_404464935.1 homoserine O-acetyltransferase -
  LG331_RS01730 (LG331_01725) - 353849..354442 (-) 594 WP_311065758.1 YggT family protein -
  LG331_RS01735 (LG331_01730) proC 354543..355370 (-) 828 WP_399844009.1 pyrroline-5-carboxylate reductase -
  LG331_RS01740 (LG331_01735) - 355459..356157 (-) 699 WP_404464937.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG331_RS01745 (LG331_01740) pilT 356191..357234 (+) 1044 WP_253019159.1 type IV pilus twitching motility protein PilT Machinery gene
  LG331_RS01750 (LG331_01745) pilU 357259..358419 (+) 1161 WP_404464939.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG331_RS01755 (LG331_01750) - 358432..359574 (-) 1143 WP_404466372.1 anhydro-N-acetylmuramic acid kinase -
  LG331_RS01760 (LG331_01755) tyrS 359691..360890 (+) 1200 WP_399844014.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38233.80 Da        Isoelectric Point: 6.5960

>NTDB_id=615994 LG331_RS01745 WP_253019159.1 356191..357234(+) (pilT) [Vreelandella aquamarina strain ADH2-948]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMENSDVRRLIYDIMNDRQRRDYEEHLEADFSFEVPGVAR
FRVNAFNQARGAGAVFRTIPNKVLSMDTLGMGEVFQRLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINEQRFEHILTIED
PVEFVHTSKRCLINQREVHRDTHSFSGALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKSMVRSMLSESLQAVVSQTLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQIYSAIQTGGALGMQTLNASL
AKLVKEGVVSMEEAQARAKGTLTLKDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=615994 LG331_RS01745 WP_253019159.1 356191..357234(+) (pilT) [Vreelandella aquamarina strain ADH2-948]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAATGCATCCGACCTGCACCTTTCGGCGGGTCTGCCTCCCAT
GATACGTGTTGATGGCGATATTCGTCGGCTTAATGTGCCCGCCATGGAGAACAGCGACGTTCGGCGGCTGATCTACGACA
TCATGAACGATCGGCAGCGCCGCGACTATGAAGAGCACTTAGAAGCGGACTTCTCGTTTGAAGTGCCGGGGGTGGCGCGC
TTTAGGGTTAATGCCTTCAATCAGGCACGCGGGGCGGGGGCGGTATTCCGTACCATTCCCAATAAGGTGCTTTCCATGGA
CACCCTGGGCATGGGCGAAGTGTTCCAACGCCTTTCGATGCTACCCAGGGGATTAGTGCTGGTGACCGGGCCCACGGGGT
CGGGCAAAAGCACGACCTTGGCGGCGATGATCGACTACATCAACGAACAGCGCTTCGAGCATATTCTGACCATCGAAGAC
CCGGTGGAGTTTGTGCACACTAGCAAACGCTGCCTGATCAATCAGCGGGAAGTTCACCGCGATACCCATAGCTTCTCTGG
GGCGCTGCGAAGCGCGCTGCGCGAAGACCCGGACGTGATTTTGGTCGGCGAGCTTCGTGATCTCGAAACAATCCGCTTAG
CGCTCACCGCTGCTGAAACGGGGCATTTGGTATTCGGCACCTTGCACACCACTTCCGCGGCGAAAACCATCGATCGGATC
ATCGATGTATTCCCCGGCGAAGAGAAATCCATGGTGCGCTCGATGCTGTCTGAATCGCTCCAGGCGGTTGTCTCGCAAAC
ACTATTGAAGCGTCAGGGTGGCGGACGCGTGGCGGCCCACGAGATCCTGATTGCCACGTCTGCGGTGCGTAACCTCATTC
GCGAAGATAAAGTGGCGCAGATCTACTCCGCCATCCAGACCGGCGGTGCACTGGGAATGCAGACCCTGAATGCCTCGCTC
GCCAAGCTGGTTAAAGAGGGCGTGGTGAGTATGGAGGAAGCCCAAGCAAGGGCTAAAGGCACGCTGACGTTAAAAGATGA
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

79.351

97.695

0.775
  pilT Pseudomonas stutzeri DSM 10701

78.761

97.695

0.769
  pilT Acinetobacter nosocomialis M2

77.581

97.695

0.758
  pilT Acinetobacter baumannii D1279779

77.581

97.695

0.758
  pilT Acinetobacter baumannii strain A118

77.581

97.695

0.758
  pilT Acinetobacter baylyi ADP1

74.926

97.695

0.732
  pilT Legionella pneumophila strain Lp02

71.598

97.406

0.697
  pilT Legionella pneumophila strain ERS1305867

71.598

97.406

0.697
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.623

97.118

0.686
  pilT Vibrio cholerae strain A1552

70.623

97.118

0.686
  pilT Neisseria meningitidis 8013

67.251

98.559

0.663
  pilT Neisseria gonorrhoeae MS11

66.959

98.559

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.687

97.695

0.524
  pilU Pseudomonas stutzeri DSM 10701

40.462

99.712

0.403
  pilU Acinetobacter baylyi ADP1

39.481

100

0.395
  pilU Vibrio cholerae strain A1552

40.299

96.542

0.389