Detailed information NTG0615995

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LG331_RS01750
• Genome accession: NZ_CP085222
• Coordinates: 357259..358419 (+)
• Length: 386 a.a.
• NCBI ID: WP_404464939.1
• Uniprot ID: -
• Organism: Vreelandella aquamarina strain ADH2-948
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 352259..363419

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LG331_RS01725 (LG331_01720)	-	352579..353775 (-)	1197	WP_404464935.1	homoserine O-acetyltransferase	-
LG331_RS01730 (LG331_01725)	-	353849..354442 (-)	594	WP_311065758.1	YggT family protein	-
LG331_RS01735 (LG331_01730)	proC	354543..355370 (-)	828	WP_399844009.1	pyrroline-5-carboxylate reductase	-
LG331_RS01740 (LG331_01735)	-	355459..356157 (-)	699	WP_404464937.1	YggS family pyridoxal phosphate-dependent enzyme	-
LG331_RS01745 (LG331_01740)	pilT	356191..357234 (+)	1044	WP_253019159.1	type IV pilus twitching motility protein PilT	Machinery gene
LG331_RS01750 (LG331_01745)	pilU	357259..358419 (+)	1161	WP_404464939.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LG331_RS01755 (LG331_01750)	-	358432..359574 (-)	1143	WP_404466372.1	anhydro-N-acetylmuramic acid kinase	-
LG331_RS01760 (LG331_01755)	tyrS	359691..360890 (+)	1200	WP_399844014.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 42012.01 Da
• Isoelectric Point: 6.8279

>NTDB_id=615995 LG331_RS01750 WP_404464939.1 357259..358419(+) (pilU) [Vreelandella aquamarina strain ADH2-948]
MSEPASGMTATQWLHQLLGIMVEQQASDLLISVGAPPSLKTPKGLMPLGRQPLSSSQVRSLVLNAVPEKQRERFQEEHEA
NFALSLESAGRFRVSAFQQRNEPAMVVRRIAQTIPTLSALGVPSQLATLANAKRGLVLVVGGTGTGKSTTLAAMIQERNA
SVGGHIITIEDPIEYLHPHQRGIVNQREVGVDTESFEVALKNTLRQAPDVILIGEVRTRETMEHALTFAETGHLCLATLH
ANNANQALERILHFFPHERHEQVLMDLSLNLRAVVAQQLLPTQQGGRCAAIEILLSSPRVVDLIRKGRIDELKQAMAGSR
DAGMQTFDQALYALYQAGQISQQVALAHADSANDLRLMIKQGDAASGKPLDAQVPSHLALRDGDDY

Nucleotide

• Length: 1161 bp

>NTDB_id=615995 LG331_RS01750 WP_404464939.1 357259..358419(+) (pilU) [Vreelandella aquamarina strain ADH2-948]
ATGAGTGAGCCAGCCAGCGGTATGACCGCGACCCAGTGGCTGCACCAACTGCTGGGCATCATGGTCGAGCAGCAGGCCTC
CGACCTGCTGATTTCGGTGGGCGCGCCGCCCAGCTTGAAAACGCCCAAGGGGCTGATGCCGCTGGGGCGTCAGCCGCTCT
CCTCTAGCCAGGTGCGCAGCCTGGTGCTGAATGCGGTTCCAGAGAAGCAGCGGGAGCGATTTCAAGAGGAGCACGAGGCG
AATTTTGCCCTAAGCCTGGAGAGCGCGGGGCGGTTTCGGGTAAGCGCCTTTCAACAGCGTAACGAGCCCGCCATGGTGGT
GCGCCGTATTGCGCAGACCATTCCAACCCTCAGCGCGCTGGGCGTGCCTAGCCAGTTGGCGACGCTGGCCAATGCCAAGC
GCGGGCTGGTGCTGGTGGTGGGCGGCACGGGAACGGGGAAATCCACCACCCTGGCGGCAATGATTCAGGAGCGTAACGCC
TCAGTCGGCGGGCATATCATCACCATTGAGGACCCCATCGAGTACTTGCATCCGCACCAGCGTGGCATCGTTAATCAGCG
CGAAGTGGGGGTTGATACGGAGTCCTTCGAGGTGGCGCTGAAAAATACGCTACGCCAAGCGCCGGATGTGATCTTGATTG
GCGAAGTGCGTACCCGCGAGACCATGGAGCACGCCCTCACTTTCGCGGAAACCGGGCATCTTTGTCTGGCGACATTGCAC
GCCAACAACGCCAACCAAGCCTTAGAGCGCATCCTTCACTTCTTTCCCCATGAGCGGCACGAGCAGGTGCTGATGGATCT
ATCGCTGAATCTGCGTGCCGTAGTGGCGCAGCAATTGCTGCCTACTCAGCAGGGTGGCCGCTGCGCGGCGATCGAGATTT
TGCTCTCATCGCCGCGGGTGGTGGATCTGATCCGTAAAGGGCGTATTGATGAATTGAAACAGGCCATGGCAGGCTCTCGC
GATGCGGGGATGCAAACTTTCGATCAGGCGCTTTATGCGCTCTATCAAGCGGGCCAAATCAGCCAGCAAGTGGCGCTGGC
CCATGCGGATTCCGCCAACGACCTGCGGCTGATGATCAAGCAGGGCGACGCCGCCAGCGGTAAGCCGTTAGACGCGCAGG
TGCCTTCTCATCTTGCTCTACGCGACGGTGACGATTATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (50-281)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.126	96.632	0.591
pilU	Vibrio cholerae strain A1552	59.49	91.451	0.544
pilU	Acinetobacter baylyi ADP1	56.389	93.264	0.526
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.4	97.15	0.412
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	42.683	84.974	0.363
pilT	Vibrio cholerae strain A1552	42.683	84.974	0.363
pilT	Legionella pneumophila strain Lp02	42.121	85.492	0.36
pilT	Legionella pneumophila strain ERS1305867	42.121	85.492	0.36
pilT	Neisseria meningitidis 8013	40.643	88.601	0.36