Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG312_RS10460 Genome accession   NZ_CP085221
Coordinates   2278780..2279814 (+) Length   344 a.a.
NCBI ID   WP_379760453.1    Uniprot ID   -
Organism   Halomonas sp. strain DBC-59     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2273780..2284814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG312_RS10430 (LG312_10450) - 2274149..2274604 (-) 456 WP_379760463.1 DUF4426 domain-containing protein -
  LG312_RS10435 (LG312_10455) metW 2274658..2275254 (-) 597 WP_379760559.1 methionine biosynthesis protein MetW -
  LG312_RS10440 (LG312_10460) - 2275254..2276411 (-) 1158 WP_404295693.1 homoserine O-acetyltransferase -
  LG312_RS10445 (LG312_10465) - 2276454..2277035 (-) 582 WP_379760459.1 YggT family protein -
  LG312_RS10450 (LG312_10470) proC 2277099..2277923 (-) 825 WP_379760457.1 pyrroline-5-carboxylate reductase -
  LG312_RS10455 (LG312_10475) - 2277994..2278713 (-) 720 WP_379760455.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG312_RS10460 (LG312_10480) pilT 2278780..2279814 (+) 1035 WP_379760453.1 type IV pilus twitching motility protein PilT Machinery gene
  LG312_RS10465 (LG312_10485) pilU 2279866..2281008 (+) 1143 WP_379760451.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG312_RS10470 (LG312_10490) - 2281027..2282166 (-) 1140 WP_404295696.1 anhydro-N-acetylmuramic acid kinase -
  LG312_RS10475 (LG312_10495) tyrS 2282281..2283480 (+) 1200 WP_379760447.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37961.57 Da        Isoelectric Point: 6.5957

>NTDB_id=615987 LG312_RS10460 WP_379760453.1 2278780..2279814(+) (pilT) [Halomonas sp. strain DBC-59]
MDITELLAFSAKQKASDLHLSAGLPPMIRVDGDIRRLNVPAMEDREVRKLIYDIMADRQRRDYEEFFETDFSFEVPGVSR
FRVNVFNHARGSGAVFRTIPTDVLTMEDLGLGTVFRQLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINENRYEHILTIED
PVEFVHRSKRCLINQREVHRDTHGFAPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGDEKSMVRSMLSESLQAVISQALLKRMGGGRVAAHEILIATAAVRNLVREDKVAQIYSAIQTGGNLGMQTLDASL
AKLVADNVVARDEAQAKAKGVLAN

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=615987 LG312_RS10460 WP_379760453.1 2278780..2279814(+) (pilT) [Halomonas sp. strain DBC-59]
ATGGATATTACCGAACTGCTGGCGTTCTCGGCAAAACAGAAGGCCTCTGACCTGCATCTTTCGGCAGGCCTGCCGCCGAT
GATCCGGGTCGACGGCGACATTCGTCGTCTGAACGTACCGGCCATGGAGGACCGCGAGGTCCGAAAGTTGATCTATGACA
TCATGGCTGACCGCCAGCGCCGTGACTACGAGGAGTTTTTCGAGACTGACTTCTCCTTCGAGGTGCCTGGGGTGTCGCGT
TTCCGTGTCAATGTTTTCAACCATGCCCGTGGCTCCGGGGCGGTATTCCGGACCATCCCCACCGATGTGCTGACCATGGA
GGACCTCGGTCTTGGTACCGTCTTCCGCCAGCTCTCCATGTTGCCCCGTGGTCTGGTGCTGGTGACCGGCCCCACCGGTT
CAGGCAAGAGCACTACTTTGGCGGCGATGATCGATTACATCAACGAAAACCGCTACGAGCATATCCTCACCATCGAGGAT
CCAGTGGAATTCGTCCACCGCAGCAAGCGCTGTCTGATCAACCAGCGCGAGGTGCACCGAGATACCCACGGCTTCGCTCC
GGCACTGCGCAGCGCCCTGCGCGAGGATCCGGACGTGATCCTGGTAGGCGAGCTTCGCGATCTGGAAACCATCCGGCTGG
CGCTCACCGCAGCCGAAACAGGGCACCTGGTGTTCGGTACCCTGCACACCACCTCGGCGGCCAAGACCATTGACCGGATC
ATCGACGTCTTCCCCGGGGATGAAAAGTCGATGGTGCGCTCGATGCTTTCGGAGTCGCTCCAGGCGGTGATCTCCCAGGC
GCTGCTCAAACGCATGGGTGGCGGCCGTGTGGCGGCCCACGAGATCCTTATCGCCACCGCGGCGGTGCGTAACCTGGTCC
GCGAAGACAAGGTGGCGCAGATCTACTCAGCCATTCAGACCGGCGGCAACCTGGGCATGCAGACCTTGGATGCCTCTCTG
GCCAAGCTGGTGGCAGATAATGTGGTCGCGCGCGACGAGGCGCAGGCCAAGGCCAAGGGCGTGCTGGCGAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.466

98.547

0.773
  pilT Pseudomonas aeruginosa PAK

77.581

98.547

0.765
  pilT Acinetobacter baumannii D1279779

76.991

98.547

0.759
  pilT Acinetobacter baumannii strain A118

76.991

98.547

0.759
  pilT Acinetobacter nosocomialis M2

76.696

98.547

0.756
  pilT Acinetobacter baylyi ADP1

75.516

98.547

0.744
  pilT Legionella pneumophila strain Lp02

71.006

98.256

0.698
  pilT Legionella pneumophila strain ERS1305867

71.006

98.256

0.698
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.176

98.837

0.654
  pilT Neisseria gonorrhoeae MS11

65.882

98.837

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.507

98.547

0.517
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

40.923

94.477

0.387
  pilU Vibrio cholerae strain A1552

39.701

97.384

0.387