Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LG312_RS10465 Genome accession   NZ_CP085221
Coordinates   2279866..2281008 (+) Length   380 a.a.
NCBI ID   WP_379760451.1    Uniprot ID   -
Organism   Halomonas sp. strain DBC-59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2274866..2286008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG312_RS10440 (LG312_10460) - 2275254..2276411 (-) 1158 WP_404295693.1 homoserine O-acetyltransferase -
  LG312_RS10445 (LG312_10465) - 2276454..2277035 (-) 582 WP_379760459.1 YggT family protein -
  LG312_RS10450 (LG312_10470) proC 2277099..2277923 (-) 825 WP_379760457.1 pyrroline-5-carboxylate reductase -
  LG312_RS10455 (LG312_10475) - 2277994..2278713 (-) 720 WP_379760455.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG312_RS10460 (LG312_10480) pilT 2278780..2279814 (+) 1035 WP_379760453.1 type IV pilus twitching motility protein PilT Machinery gene
  LG312_RS10465 (LG312_10485) pilU 2279866..2281008 (+) 1143 WP_379760451.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG312_RS10470 (LG312_10490) - 2281027..2282166 (-) 1140 WP_404295696.1 anhydro-N-acetylmuramic acid kinase -
  LG312_RS10475 (LG312_10495) tyrS 2282281..2283480 (+) 1200 WP_379760447.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42230.57 Da        Isoelectric Point: 5.9736

>NTDB_id=615988 LG312_RS10465 WP_379760451.1 2279866..2281008(+) (pilU) [Halomonas sp. strain DBC-59]
MTAKEWLYQLLDIMVQKEASDLLISVNAPPTLKMAGKLTPLGDHNLSVEQVTELVGTSIPDVLRERFRVEREANFALSLK
GKGRFRVSAFQQRNQMAMVIRRIGFEIPHLEELSLPSTLGELVNNKRGLVFVVGGTGTGKSTTLASMIQYRNETIGGHII
SVEDPIEYVHPHKKAIINQREVGIDTESFEVALKNTLRQAPDVILIGEVRTQETMEHALTFAETGHLCLATLHANNANQA
LDRIINFFPIERHAQVWLDLSLNLRAIVAQQLLPTVDGGRCPAIEIMLKSPLIGDLIRKGDVSEIKNVMTRSRDLGMQTF
DQALFDLFKEGKITEEVALVHADSANDLRMMIKYGDAGSDGMTQAVEAASHLSLKRDDDF

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=615988 LG312_RS10465 WP_379760451.1 2279866..2281008(+) (pilU) [Halomonas sp. strain DBC-59]
ATGACGGCGAAAGAGTGGCTCTATCAGCTGCTGGATATCATGGTGCAGAAGGAAGCATCGGATTTGCTGATTTCGGTGAA
TGCACCGCCAACGCTGAAAATGGCCGGTAAGCTCACCCCCCTCGGAGATCATAATCTCAGCGTGGAGCAGGTCACCGAAC
TGGTCGGCACATCGATTCCCGACGTCTTGCGGGAACGCTTCCGGGTCGAACGCGAAGCCAATTTTGCGCTGAGCCTCAAG
GGCAAGGGTCGCTTTCGCGTCAGCGCCTTCCAACAGCGCAACCAGATGGCGATGGTGATCCGGCGTATCGGCTTTGAGAT
TCCGCATCTGGAGGAGCTGTCGCTGCCTTCCACGCTTGGCGAGCTGGTCAATAACAAGCGCGGTCTCGTGTTCGTGGTCG
GGGGCACCGGCACCGGCAAGTCGACCACCCTGGCGTCGATGATCCAGTATCGCAACGAGACGATAGGTGGCCATATTATC
AGTGTCGAGGACCCCATCGAGTACGTGCATCCGCACAAGAAAGCGATCATCAATCAGCGCGAAGTCGGGATCGACACTGA
ATCCTTCGAGGTGGCGCTCAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGGGAAGTGCGTACCCAGGAGA
CTATGGAGCACGCTCTGACCTTTGCCGAGACAGGCCACCTCTGCCTGGCCACGCTTCACGCCAACAATGCCAACCAGGCG
CTGGACCGCATCATCAACTTCTTTCCCATCGAGCGTCACGCACAGGTCTGGCTCGACCTCTCGCTCAACCTGCGGGCCAT
TGTCGCTCAGCAACTGCTGCCCACGGTGGACGGTGGACGCTGCCCGGCCATCGAGATCATGCTCAAGTCGCCCCTGATCG
GTGACCTGATACGCAAGGGCGACGTCAGCGAGATCAAGAATGTCATGACCCGTTCGCGCGACCTGGGCATGCAGACCTTC
GATCAGGCGCTTTTCGATCTCTTCAAGGAGGGTAAGATCACCGAGGAGGTGGCATTGGTGCATGCCGACTCGGCCAACGA
TCTGCGCATGATGATCAAGTACGGCGACGCGGGCAGCGACGGGATGACCCAGGCTGTCGAGGCCGCCAGCCACCTCTCGC
TGAAGCGCGATGATGACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(51-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.753

97.895

0.595
  pilU Vibrio cholerae strain A1552

56.593

95.789

0.542
  pilU Acinetobacter baylyi ADP1

54.261

92.632

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.388

100

0.424
  pilT Vibrio cholerae strain A1552

42.813

86.053

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.813

86.053

0.368
  pilT Neisseria meningitidis 8013

41.176

89.474

0.368
  pilT Neisseria gonorrhoeae MS11

40.882

89.474

0.366
  pilT Legionella pneumophila strain Lp02

41.566

87.368

0.363
  pilT Legionella pneumophila strain ERS1305867

41.566

87.368

0.363
  pilT Acinetobacter baylyi ADP1

40.828

88.947

0.363