Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG277_RS01940 Genome accession   NZ_CP085210
Coordinates   420146..421180 (+) Length   344 a.a.
NCBI ID   WP_290823233.1    Uniprot ID   -
Organism   Vreelandella aquamarina strain QJJ-865     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415146..426180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG277_RS01910 (LG277_01915) - 415550..416047 (-) 498 WP_290823238.1 DUF4426 domain-containing protein -
  LG277_RS01915 (LG277_01920) metW 416067..416663 (-) 597 WP_404417843.1 methionine biosynthesis protein MetW -
  LG277_RS01920 (LG277_01925) - 416663..417856 (-) 1194 WP_290823237.1 homoserine O-acetyltransferase -
  LG277_RS01925 (LG277_01930) - 417899..418492 (-) 594 WP_290823236.1 YggT family protein -
  LG277_RS01930 (LG277_01935) proC 418531..419355 (-) 825 WP_404415751.1 pyrroline-5-carboxylate reductase -
  LG277_RS01935 (LG277_01940) - 419409..420113 (-) 705 WP_404415754.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG277_RS01940 (LG277_01945) pilT 420146..421180 (+) 1035 WP_290823233.1 type IV pilus twitching motility protein PilT Machinery gene
  LG277_RS01945 (LG277_01950) pilU 421234..422391 (+) 1158 WP_404415757.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG277_RS01950 (LG277_01955) - 422412..423566 (-) 1155 WP_404415760.1 anhydro-N-acetylmuramic acid kinase -
  LG277_RS01955 (LG277_01960) tyrS 423756..424955 (+) 1200 WP_404415763.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37996.44 Da        Isoelectric Point: 6.1363

>NTDB_id=615891 LG277_RS01940 WP_290823233.1 420146..421180(+) (pilT) [Vreelandella aquamarina strain QJJ-865]
MDITELLAFSAKQNASDLHLSSGLPPMIRVDGDIRRLNVPAMENKEVRALIYDIMGDRQRRDYEEHLEVDFSFEVPGIAR
FRVNAFNHARGAGAVFRTIPSDVLSMETLGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYVNNHRYEHILTIED
PVEFVHTSKRCLINQREVHRDTHSFADALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPGEEKSMVRSMLSESLQAVVSQTLLKREGGGRVAAHEILIATSAIRNLIREDKIAQIYSAMQTSGGLGMQTLDASL
KQLVKEGLVTREDAQSRAKGELSL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=615891 LG277_RS01940 WP_290823233.1 420146..421180(+) (pilT) [Vreelandella aquamarina strain QJJ-865]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAACAGAATGCCTCTGACCTGCATCTGTCGTCTGGTCTTCCGCCCAT
GATACGCGTTGACGGCGATATTCGCCGGCTCAATGTGCCTGCCATGGAAAACAAGGAAGTCCGCGCCCTGATCTACGACA
TTATGGGCGATCGCCAGCGGCGTGACTACGAAGAGCACCTGGAAGTGGATTTTTCATTCGAAGTGCCGGGGATTGCGCGC
TTCAGGGTTAACGCCTTTAATCACGCCAGGGGGGCTGGTGCCGTGTTCCGCACCATTCCAAGCGACGTATTGTCCATGGA
AACGCTGGGACTGGGCGAAGTCTTTGAACGCCTGGCCATGCTGCCACGTGGGCTGGTGCTGGTGACAGGCCCCACTGGCT
CGGGCAAAAGCACCACACTGGCAGCGATGATTGACTACGTTAACAATCACCGATACGAGCATATCCTGACGATCGAGGAT
CCGGTGGAGTTTGTGCATACCAGCAAGCGTTGCCTGATCAATCAGCGTGAAGTCCACCGTGACACCCATAGCTTTGCAGA
TGCCTTGCGAAGTGCCTTGCGCGAAGACCCGGATGTCATCCTGGTCGGCGAGTTGCGCGATCTTGAAACGATTCGTCTGG
CCCTGACGGCCGCTGAAACCGGGCATCTCGTTTTTGGTACCCTGCATACCACGTCGGCGGCGAAGACGATTGACCGTATA
GTCGATGTCTTCCCCGGCGAGGAAAAATCCATGGTGCGTTCAATGCTCTCCGAATCCCTTCAGGCGGTAGTCTCTCAGAC
GCTGCTCAAGCGAGAGGGAGGCGGGCGTGTGGCCGCTCATGAAATCCTGATCGCCACCTCGGCTATTCGTAATCTCATCC
GCGAAGACAAGATTGCGCAGATCTACTCGGCCATGCAAACCAGCGGGGGGTTGGGAATGCAAACTCTGGATGCCTCACTA
AAACAGTTGGTGAAAGAAGGTCTGGTCACTCGGGAAGATGCCCAGTCGCGTGCCAAGGGCGAGCTTTCCCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.646

98.547

0.785
  pilT Pseudomonas aeruginosa PAK

79.351

98.547

0.782
  pilT Acinetobacter baylyi ADP1

74.709

100

0.747
  pilT Acinetobacter baumannii strain A118

75.516

98.547

0.744
  pilT Acinetobacter nosocomialis M2

75.516

98.547

0.744
  pilT Acinetobacter baumannii D1279779

75.516

98.547

0.744
  pilT Legionella pneumophila strain Lp02

71.302

98.256

0.701
  pilT Legionella pneumophila strain ERS1305867

71.302

98.256

0.701
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.421

99.419

0.68
  pilT Vibrio cholerae strain A1552

68.421

99.419

0.68
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.392

98.547

0.526
  pilU Pseudomonas stutzeri DSM 10701

42.388

97.384

0.413
  pilU Vibrio cholerae strain A1552

42.09

97.384

0.41
  pilU Acinetobacter baylyi ADP1

40.588

98.837

0.401