Detailed information NTG0615892

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LG277_RS01945
• Genome accession: NZ_CP085210
• Coordinates: 421234..422391 (+)
• Length: 385 a.a.
• NCBI ID: WP_404415757.1
• Uniprot ID: -
• Organism: Vreelandella aquamarina strain QJJ-865
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 416234..427391

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LG277_RS01920 (LG277_01925)	-	416663..417856 (-)	1194	WP_290823237.1	homoserine O-acetyltransferase	-
LG277_RS01925 (LG277_01930)	-	417899..418492 (-)	594	WP_290823236.1	YggT family protein	-
LG277_RS01930 (LG277_01935)	proC	418531..419355 (-)	825	WP_404415751.1	pyrroline-5-carboxylate reductase	-
LG277_RS01935 (LG277_01940)	-	419409..420113 (-)	705	WP_404415754.1	YggS family pyridoxal phosphate-dependent enzyme	-
LG277_RS01940 (LG277_01945)	pilT	420146..421180 (+)	1035	WP_290823233.1	type IV pilus twitching motility protein PilT	Machinery gene
LG277_RS01945 (LG277_01950)	pilU	421234..422391 (+)	1158	WP_404415757.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LG277_RS01950 (LG277_01955)	-	422412..423566 (-)	1155	WP_404415760.1	anhydro-N-acetylmuramic acid kinase	-
LG277_RS01955 (LG277_01960)	tyrS	423756..424955 (+)	1200	WP_404415763.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 42391.34 Da
• Isoelectric Point: 5.8723

>NTDB_id=615892 LG277_RS01945 WP_404415757.1 421234..422391(+) (pilU) [Vreelandella aquamarina strain QJJ-865]
MSQSVTPTEWLHQLLDIMVEQQASDLLVSVGAPPSLKTPDGLVPLGQQGLNDHQVRELVMHTLPEGLRSRFQQEREANFA
LSIEDKGRFRVSAFQQRNQLAMVVRRISHEIPKLEQLGMPAMLASLAQENRGLVLVVGGTGTGKSTTLAAMIQQRNETLG
GHIISVEDPIEYLHPHKRAIINQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHLCLATLHANN
ANQALERIIHFFPHERHEQIWMDLSLNLKAVVAQQLLPDQAGGRSAAVEIMLRSPRIADLISKGDVGEIKSAMAKSRDDG
MQTFDQALFDLFNAGKVSADVALAHADSANDLRMMMKFGGADSKAATGLESRVPSGLALRDEDDY

Nucleotide

• Length: 1158 bp

>NTDB_id=615892 LG277_RS01945 WP_404415757.1 421234..422391(+) (pilU) [Vreelandella aquamarina strain QJJ-865]
ATGAGCCAAAGCGTGACGCCCACAGAATGGTTACACCAGCTGCTGGATATCATGGTCGAACAGCAGGCGTCGGACCTCCT
GGTGTCCGTGGGTGCGCCACCCAGCCTGAAAACGCCTGACGGTCTGGTGCCGCTTGGCCAGCAGGGCCTGAATGACCATC
AGGTAAGGGAGTTGGTGATGCATACCCTTCCTGAAGGCCTGCGCTCGCGTTTTCAGCAGGAGCGTGAAGCCAATTTTGCG
CTGAGCATTGAAGATAAAGGCCGCTTCAGGGTCAGCGCCTTCCAGCAGCGCAACCAGTTGGCAATGGTGGTGCGGCGCAT
CAGCCATGAAATTCCCAAGCTTGAACAGTTGGGCATGCCAGCAATGCTGGCCTCCCTGGCACAGGAGAATCGCGGCCTGG
TACTGGTGGTCGGCGGGACCGGTACGGGCAAGTCGACGACCCTGGCGGCCATGATCCAGCAGCGCAATGAAACACTGGGT
GGACACATAATCAGCGTTGAAGATCCGATCGAGTATCTCCACCCCCACAAGCGGGCGATTATCAATCAGCGCGAAGTCGG
GATTGATACTGAATCCTTTGAAGTTGCCCTGAAGAACACCTTGCGTCAGGCGCCTGATGTGATTTTGATTGGTGAGATTC
GCACGCGAGAAACCATGGAGCATGCGCTGACGTTTGCCGAGACAGGTCATTTATGCCTGGCGACCTTGCACGCTAACAAT
GCTAACCAGGCGCTGGAGCGGATTATCCACTTTTTCCCTCATGAACGTCACGAACAGATCTGGATGGACTTGTCGCTTAA
TCTGAAGGCGGTGGTGGCACAGCAGTTGCTGCCTGATCAAGCTGGCGGTCGCTCGGCCGCTGTAGAGATCATGCTGCGCT
CGCCACGCATTGCCGACCTGATCAGTAAAGGTGACGTGGGCGAGATCAAGTCTGCTATGGCGAAATCCCGTGATGATGGT
ATGCAGACGTTTGATCAGGCACTTTTTGATCTTTTCAATGCGGGTAAGGTCAGTGCTGACGTTGCGCTGGCGCATGCCGA
TTCTGCCAATGACTTACGCATGATGATGAAATTCGGTGGCGCCGATAGTAAAGCGGCCACTGGCCTGGAAAGCAGGGTGC
CCAGTGGATTGGCATTGCGTGATGAAGACGATTATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (74-277)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.181	97.662	0.558
pilU	Vibrio cholerae strain A1552	56.906	94.026	0.535
pilU	Acinetobacter baylyi ADP1	53.933	92.468	0.499
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.257	95.065	0.392