Detailed information NTG0615841

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: IUX27_RS04215
• Genome accession: NZ_CP085202
• Coordinates: 881871..882851 (+)
• Length: 326 a.a.
• NCBI ID: WP_001055626.1
• Uniprot ID: -
• Organism: Shigella flexneri strain P099
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 876871..887851

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IUX27_RS04185 (IUX27_04185)	-	877271..878234 (-)	964	Protein_800	TRAP transporter substrate-binding protein	-
IUX27_RS04190 (IUX27_04190)	hemW	878548..879684 (-)	1137	WP_000239924.1	radical SAM family heme chaperone HemW	-
IUX27_RS04195 (IUX27_04195)	-	879677..880270 (-)	594	WP_001174735.1	XTP/dITP diphosphatase	-
IUX27_RS04200 (IUX27_04200)	yggU	880278..880568 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
IUX27_RS04205 (IUX27_04205)	yggT	880565..881131 (-)	567	WP_001094817.1	osmotic shock tolerance protein YggT	-
IUX27_RS04210 (IUX27_04210)	yggS	881149..881853 (-)	705	WP_000997800.1	pyridoxal phosphate homeostasis protein	-
IUX27_RS04215 (IUX27_04215)	pilT	881871..882851 (+)	981	WP_001055626.1	type IV pilus twitching motility protein PilT	Machinery gene
IUX27_RS04220 (IUX27_04220)	ruvX	883027..883443 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
IUX27_RS04225 (IUX27_04225)	-	883443..884006 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
IUX27_RS04230 (IUX27_04230)	gshB	884115..885065 (-)	951	WP_000593267.1	glutathione synthase	-
IUX27_RS04235 (IUX27_04235)	rsmE	885078..885809 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
IUX27_RS04240 (IUX27_04240)	endA	885889..886596 (-)	708	WP_000286517.1	deoxyribonuclease I	-
IUX27_RS04245 (IUX27_04245)	-	886691..887188 (-)	498	WP_005051770.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35942.17 Da
• Isoelectric Point: 5.9975

>NTDB_id=615841 IUX27_RS04215 WP_001055626.1 881871..882851(+) (pilT) [Shigella flexneri strain P099]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=615841 IUX27_RS04215 WP_001055626.1 881871..882851(+) (pilT) [Shigella flexneri strain P099]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCTACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTTGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGAAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	45.26	100	0.454
pilT	Legionella pneumophila strain Lp02	45.26	100	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	39.514	100	0.399
pilU	Pseudomonas stutzeri DSM 10701	37.576	100	0.38
pilU	Acinetobacter baylyi ADP1	36.957	98.773	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362