Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG291_RS19805 Genome accession   NZ_CP084990
Coordinates   3887568..3888623 (-) Length   351 a.a.
NCBI ID   WP_404355665.1    Uniprot ID   -
Organism   Cytobacillus firmus strain WLG-456     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3882568..3893623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG291_RS19780 (LG291_19615) - 3883341..3884000 (-) 660 WP_404355656.1 fimbrial assembly protein -
  LG291_RS19785 (LG291_19620) pilM 3884004..3884999 (-) 996 WP_404355657.1 type IV pilus biogenesis protein PilM -
  LG291_RS19790 (LG291_19625) comC 3885088..3885840 (-) 753 WP_404355659.1 prepilin peptidase Machinery gene
  LG291_RS19795 (LG291_19630) - 3885851..3886270 (-) 420 WP_404355661.1 type II secretion system protein -
  LG291_RS19800 (LG291_19635) - 3886376..3887581 (-) 1206 WP_404355663.1 type II secretion system F family protein -
  LG291_RS19805 (LG291_19640) pilT 3887568..3888623 (-) 1056 WP_404355665.1 type IV pilus twitching motility protein PilT Machinery gene
  LG291_RS19810 (LG291_19645) - 3888633..3890294 (-) 1662 WP_394580162.1 GspE/PulE family protein -
  LG291_RS19815 (LG291_19650) - 3890316..3891689 (-) 1374 WP_404355667.1 VanW family protein -
  LG291_RS19820 (LG291_19655) - 3891709..3892443 (-) 735 WP_135147936.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38903.11 Da        Isoelectric Point: 9.1537

>NTDB_id=614227 LG291_RS19805 WP_404355665.1 3887568..3888623(-) (pilT) [Cytobacillus firmus strain WLG-456]
MKTKIDYLLRAAYELKASDLHLTVGVPPVIRINGDLKKYGKESLRPEDTEGMAKVIIPANIWKKFEENGELDFSYGIPGL
SRFRVNAYFQRSCISLAIRVVPTRIPDLEELGLPAVLKKVAEKPQGLVLVTGPTGSGKSTTLASMIHYMNETMRKHIITL
EDPIEYLHKHGNCIIDQREVGFDTKHFANGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTSSAPATIN
RIIDVFPPSQQAQIRIQLASVLVSIISQRLFLTADRKGRHAATEVLMNNSAVANLIRNEKIYQIINVMQTSRAQGMHTLE
TSITSLLESGKIAKESALPYIKEKVTDHAPL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=614227 LG291_RS19805 WP_404355665.1 3887568..3888623(-) (pilT) [Cytobacillus firmus strain WLG-456]
ATGAAGACAAAAATAGACTATCTGTTGAGGGCTGCGTATGAGCTGAAGGCTTCGGATTTGCATTTGACCGTTGGGGTTCC
TCCTGTCATACGGATTAATGGTGATTTAAAAAAATACGGGAAAGAAAGTTTGCGTCCGGAAGATACCGAGGGAATGGCTA
AAGTCATTATTCCTGCGAACATATGGAAAAAGTTTGAGGAGAATGGCGAGCTGGATTTCTCTTATGGAATACCCGGGCTA
TCCCGTTTCAGAGTCAATGCTTATTTTCAGCGGTCATGCATTTCACTGGCCATCCGTGTTGTTCCGACTCGAATTCCTGA
CCTGGAAGAACTTGGGCTTCCAGCTGTCTTAAAGAAAGTGGCGGAAAAGCCTCAGGGACTGGTCCTGGTGACAGGGCCAA
CGGGAAGCGGAAAATCAACGACATTAGCTTCGATGATTCATTACATGAACGAGACAATGAGGAAGCATATTATTACGCTT
GAGGACCCGATTGAATATCTTCATAAGCATGGAAATTGCATTATTGACCAGCGGGAAGTTGGGTTTGATACAAAGCATTT
TGCAAATGGATTAAGAGCTGCTTTGCGTCAGGATCCTGATGTGATACTTGTAGGGGAAATGCGTGACCTGGAAACCATCC
AAACAGCCATAACAGCAGCCGAAACTGGCCATTTAGTGCTTGGCACTCTGCATACCTCAAGTGCTCCAGCTACGATTAAC
CGAATTATCGATGTATTTCCGCCATCCCAGCAGGCGCAAATCAGAATACAGCTTGCCTCTGTATTAGTGTCGATTATATC
GCAGCGCCTTTTTCTCACAGCAGACAGGAAGGGCCGACATGCAGCTACAGAAGTGCTTATGAATAATTCTGCTGTGGCGA
ACCTGATCAGAAATGAAAAGATCTATCAGATTATTAATGTGATGCAAACGTCCCGCGCCCAGGGAATGCATACACTCGAG
ACAAGTATTACTTCTCTATTGGAGAGCGGAAAAATCGCCAAAGAATCAGCATTGCCGTACATAAAGGAAAAGGTGACTGA
TCATGCCCCGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.294

96.866

0.487
  pilT Neisseria meningitidis 8013

49.401

95.157

0.47
  pilT Neisseria gonorrhoeae MS11

49.102

95.157

0.467
  pilT Legionella pneumophila strain Lp02

48.649

94.872

0.462
  pilT Legionella pneumophila strain ERS1305867

48.649

94.872

0.462
  pilT Vibrio cholerae strain A1552

49.085

93.447

0.459
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.085

93.447

0.459
  pilT Pseudomonas aeruginosa PAK

48.048

94.872

0.456
  pilT Pseudomonas stutzeri DSM 10701

47.147

94.872

0.447
  pilT Acinetobacter baumannii D1279779

46.018

96.581

0.444
  pilT Acinetobacter baumannii strain A118

46.018

96.581

0.444
  pilT Acinetobacter nosocomialis M2

46.018

96.581

0.444
  pilT Acinetobacter baylyi ADP1

47.256

93.447

0.442
  pilU Pseudomonas stutzeri DSM 10701

43.284

95.442

0.413
  pilU Acinetobacter baylyi ADP1

40.719

95.157

0.387
  pilU Vibrio cholerae strain A1552

41.796

92.023

0.385