Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LF844_RS01415 Genome accession   NZ_CP084625
Coordinates   295348..296382 (+) Length   344 a.a.
NCBI ID   WP_151131312.1    Uniprot ID   A0A5J6QFS6
Organism   Metapseudomonas lalkuanensis strain ACYW.190     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 290348..301382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LF844_RS01390 (LF844_01390) - 290561..292549 (-) 1989 WP_226283277.1 dynamin-like GTPase family protein -
  LF844_RS01395 (LF844_01395) - 292759..293055 (-) 297 WP_151131309.1 DUF167 domain-containing protein -
  LF844_RS01400 (LF844_01400) - 293055..293648 (-) 594 WP_120653589.1 YggT family protein -
  LF844_RS01405 (LF844_01405) proC 293659..294477 (-) 819 WP_226283278.1 pyrroline-5-carboxylate reductase -
  LF844_RS01410 (LF844_01410) - 294492..295184 (-) 693 WP_151131311.1 YggS family pyridoxal phosphate-dependent enzyme -
  LF844_RS01415 (LF844_01415) pilT 295348..296382 (+) 1035 WP_151131312.1 type IV pilus twitching motility protein PilT Machinery gene
  LF844_RS01420 (LF844_01420) pilU 296422..297567 (+) 1146 WP_151131313.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LF844_RS01425 (LF844_01425) - 297644..298039 (+) 396 WP_151131314.1 TM2 domain-containing protein -
  LF844_RS01430 (LF844_01430) - 298277..299656 (+) 1380 WP_151131315.1 pyridoxal-phosphate dependent enzyme -
  LF844_RS01435 (LF844_01435) - 299653..300837 (+) 1185 WP_151131316.1 PLP-dependent aspartate aminotransferase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37885.68 Da        Isoelectric Point: 6.8361

>NTDB_id=612043 LF844_RS01415 WP_151131312.1 295348..296382(+) (pilT) [Metapseudomonas lalkuanensis strain ACYW.190]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEIFKKISDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSTKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQSVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLVAKGLVSRESAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=612043 LF844_RS01415 WP_151131312.1 295348..296382(+) (pilT) [Metapseudomonas lalkuanensis strain ACYW.190]
ATGGATATTACTGAGTTGCTTGCCTTCAGCGCCAAGCAGGGCGCTTCGGACCTGCATCTCTCGGCCGGCCTGCCACCTAT
GATTCGCGTCGATGGCGACGTGCGCCGGATCAACCTGCCGGCCCTGGACCACAAGCAGGTCCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCCTCGAGACCGACTTCTCCTTCGAAGTCCCTGGCGTCGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGTACCATTCCCTCCAAGGTCCTGAGCATGGA
AGACCTGGGGATGGGCGAGATCTTCAAGAAGATTTCCGATGTTCCGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCCATGCTCGATTACCTGAACAGCACCAAGTACCACCACATCCTCACCGTGGAAGAC
CCGATCGAATTCGTCCACGAGTCGAAAAAGTGCCTGATCAACCAGCGGGAAGTGCACCGCGACACCCTCGGTTTCAACGA
GGCCCTGCGTTCGGCCCTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CACTGACCGCAGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCCGCCGAGGAAAAGGCCATGGTCCGCTCCATGCTTTCCGAATCCCTCCAGTCGGTGATCTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCCATCCGTAACCTGATTC
GCGAAGACAAGGTGGCGCAGATGTACTCCGCCATCCAGACGGGCGGCTCGCTCGGCATGCAGACCCTCGACATGTGCCTC
AAGGGCCTGGTCGCCAAGGGCCTGGTCAGCCGCGAGAGCGCGCGGGAAAAGGCCAAGAGCCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6QFS6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

95.64

100

0.956
  pilT Pseudomonas stutzeri DSM 10701

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

85.756

100

0.858
  pilT Acinetobacter baumannii strain A118

85.756

100

0.858
  pilT Acinetobacter nosocomialis M2

85.465

100

0.855
  pilT Acinetobacter baylyi ADP1

83.14

100

0.831
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392