Detailed information NTG0612044

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LF844_RS01420
• Genome accession: NZ_CP084625
• Coordinates: 296422..297567 (+)
• Length: 381 a.a.
• NCBI ID: WP_151131313.1
• Uniprot ID: A0A5J6QIW8
• Organism: Metapseudomonas lalkuanensis strain ACYW.190
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 291422..302567

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LF844_RS01395 (LF844_01395)	-	292759..293055 (-)	297	WP_151131309.1	DUF167 domain-containing protein	-
LF844_RS01400 (LF844_01400)	-	293055..293648 (-)	594	WP_120653589.1	YggT family protein	-
LF844_RS01405 (LF844_01405)	proC	293659..294477 (-)	819	WP_226283278.1	pyrroline-5-carboxylate reductase	-
LF844_RS01410 (LF844_01410)	-	294492..295184 (-)	693	WP_151131311.1	YggS family pyridoxal phosphate-dependent enzyme	-
LF844_RS01415 (LF844_01415)	pilT	295348..296382 (+)	1035	WP_151131312.1	type IV pilus twitching motility protein PilT	Machinery gene
LF844_RS01420 (LF844_01420)	pilU	296422..297567 (+)	1146	WP_151131313.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LF844_RS01425 (LF844_01425)	-	297644..298039 (+)	396	WP_151131314.1	TM2 domain-containing protein	-
LF844_RS01430 (LF844_01430)	-	298277..299656 (+)	1380	WP_151131315.1	pyridoxal-phosphate dependent enzyme	-
LF844_RS01435 (LF844_01435)	-	299653..300837 (+)	1185	WP_151131316.1	PLP-dependent aspartate aminotransferase family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42623.03 Da
• Isoelectric Point: 6.8267

>NTDB_id=612044 LF844_RS01420 WP_151131313.1 296422..297567(+) (pilU) [Metapseudomonas lalkuanensis strain ACYW.190]
MEFEKLMRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNPMSPEMTRETVLGVMNEQQRRDFAEHHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETTIPTIDELKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGHRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAIAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKALMKRSSEHGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGEHLQHMSDGLSLQVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=612044 LF844_RS01420 WP_151131313.1 296422..297567(+) (pilU) [Metapseudomonas lalkuanensis strain ACYW.190]
ATGGAATTCGAGAAACTGATGCGCCTGATGGTGGAAAAAGGCGGTTCCGACCTCTTCATCACGGCGGGTGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGACCAAGAACCCCATGTCGCCGGAAATGACGCGGGAAACCGTGCTGGGCG
TGATGAACGAGCAGCAGCGCCGTGACTTCGCCGAGCACCACGAATGCAACTTCGCCATCAGCGCGCGGGGCATCGGGCGT
TTCCGTGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGGATCGAGACCACCATCCCTACCATCGA
TGAGCTGAAACTGCCGGAAATCCTCAAGAAGCTGGCACTGACCAAGCGCGGCCTGGTGCTCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCCCTGGCGGCGATGATCGGCCACCGCAACAAGAACAGCAGCGGCCACATCATTTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAGCTGCATCGTCACCCAGCGTGAAGTGGGCATCGACACCGAGTCCTTCGAAGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGGTGCGGACCCGCGAGACCATGGACCACG
CCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGCCGACCGGCAGCAGCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACTCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAAGTGCTGATCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAACTCAAGGCCCTGATGAAGCGCTCCTCCGAGCACGGCATGCAGACCTTCGACCAGGCG
CTCTACAACCTCTATTCCCAGGGCGAGATCACCTACGAAGACGCCCTGCTCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTCGGCTCGGAAACCGATGGCGAGCACTTGCAGCACATGAGCGACGGCCTGTCGCTGCAGGTGTCGGAAG
AAGACCCGGGGCGCCGCTTCCGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A5J6QIW8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	90.551	100	0.906
pilU	Acinetobacter baylyi ADP1	64.706	93.701	0.606
pilU	Vibrio cholerae strain A1552	58.571	91.864	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.345	88.189	0.391