Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LCW13_RS00980 Genome accession   NZ_CP084115
Coordinates   215264..216295 (+) Length   343 a.a.
NCBI ID   WP_043333445.1    Uniprot ID   A0AAP4TYL5
Organism   Cobetia amphilecti strain N-80     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 210264..221295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCW13_RS00955 (LCW13_00955) metW 210585..211199 (-) 615 WP_043333435.1 methionine biosynthesis protein MetW -
  LCW13_RS00960 (LCW13_00960) - 211192..212385 (-) 1194 WP_043333508.1 homoserine O-acetyltransferase -
  LCW13_RS00965 (LCW13_00965) - 212654..213235 (-) 582 WP_043333437.1 YggT family protein -
  LCW13_RS00970 (LCW13_00970) proC 213385..214227 (-) 843 WP_043333440.1 pyrroline-5-carboxylate reductase -
  LCW13_RS00975 (LCW13_00975) - 214316..215068 (-) 753 WP_043333442.1 YggS family pyridoxal phosphate-dependent enzyme -
  LCW13_RS00980 (LCW13_00980) pilT 215264..216295 (+) 1032 WP_043333445.1 type IV pilus twitching motility protein PilT Machinery gene
  LCW13_RS00985 (LCW13_00985) pilU 216435..217589 (+) 1155 WP_043333447.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LCW13_RS00990 (LCW13_00990) - 217696..218844 (-) 1149 WP_043333449.1 anhydro-N-acetylmuramic acid kinase -
  LCW13_RS00995 (LCW13_00995) tyrS 219000..220208 (+) 1209 WP_043333452.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37712.34 Da        Isoelectric Point: 6.0550

>NTDB_id=609654 LCW13_RS00980 WP_043333445.1 215264..216295(+) (pilT) [Cobetia amphilecti strain N-80]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINVPSMDDKQVRALIYEIMNDKQRRDFEEFLETDFSFEVPGVSR
FRVNAFHQGRGAGAVFRTIPSEVLTMEDLGLGEAFRKMCMLPRGLILVTGPTGSGKSTTLAAMVDYINENKYDHILTIED
PVEFVHQSKRCLVNQREVHRDTHGFAPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSATKTVDRI
IDVFPGEEKSMVRSMLSESLQGVIAQTLLKKIGGGRVAAHEIMIATPAIRNLIREDKVAQMYSSIQTGGALGMQTLDAAL
TRLLAEGLVTNEDAQAKAKGLLS

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=609654 LCW13_RS00980 WP_043333445.1 215264..216295(+) (pilT) [Cobetia amphilecti strain N-80]
ATGGATATTACCGAACTGCTGGCTTTTTCGGCAAAACAGAACGCGTCGGATCTTCACCTGTCCGCTGGTTTGCCACCGAT
GATTCGTGTCGACGGTGATGTGCGGCGCATCAATGTGCCGTCAATGGATGACAAGCAGGTGCGCGCACTGATCTACGAGA
TCATGAACGACAAGCAGCGCCGTGACTTCGAGGAATTCCTCGAGACCGATTTCTCCTTCGAGGTGCCGGGCGTCTCGCGC
TTTCGTGTCAACGCCTTCCATCAGGGGCGTGGCGCGGGGGCCGTCTTCCGTACCATCCCCTCGGAAGTGCTGACGATGGA
GGATCTGGGGCTCGGTGAAGCCTTCCGCAAGATGTGCATGCTGCCGCGCGGCCTGATTCTTGTCACCGGGCCGACCGGCT
CCGGTAAATCCACCACGCTGGCGGCGATGGTCGATTACATCAACGAGAACAAGTACGACCACATCCTCACCATCGAGGAC
CCGGTGGAATTCGTGCACCAGTCCAAGCGGTGTCTGGTCAACCAGCGTGAAGTGCACCGTGATACCCACGGCTTCGCGCC
GGCGCTGCGCTCGGCGCTGCGTGAAGACCCGGATGTCATTCTGGTCGGCGAGCTGCGAGATCTCGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACGGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCGACCAAGACCGTCGACCGTATC
ATCGACGTCTTCCCCGGCGAGGAGAAGTCGATGGTGCGCTCGATGCTGTCCGAGTCCCTGCAGGGCGTCATCGCCCAGAC
ACTGCTCAAGAAGATCGGCGGTGGCCGGGTGGCGGCTCACGAGATCATGATCGCGACGCCTGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCCATCCAGACCGGTGGCGCCCTGGGCATGCAGACACTGGATGCAGCGCTG
ACGCGTCTGTTGGCGGAAGGGCTGGTGACCAATGAAGATGCTCAGGCGAAAGCCAAGGGCTTGTTGAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

84.071

98.834

0.831
  pilT Pseudomonas aeruginosa PAK

82.301

98.834

0.813
  pilT Acinetobacter baumannii D1279779

78.466

98.834

0.776
  pilT Acinetobacter baumannii strain A118

78.466

98.834

0.776
  pilT Acinetobacter nosocomialis M2

78.171

98.834

0.773
  pilT Acinetobacter baylyi ADP1

77.876

98.834

0.77
  pilT Legionella pneumophila strain Lp02

72.781

98.542

0.717
  pilT Legionella pneumophila strain ERS1305867

72.781

98.542

0.717
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

98.251

0.691
  pilT Vibrio cholerae strain A1552

70.326

98.251

0.691
  pilT Neisseria meningitidis 8013

65.689

99.417

0.653
  pilT Neisseria gonorrhoeae MS11

65.396

99.417

0.65
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.327

98.834

0.507
  pilU Pseudomonas stutzeri DSM 10701

42.985

97.668

0.42
  pilU Vibrio cholerae strain A1552

41.194

97.668

0.402
  pilU Acinetobacter baylyi ADP1

39.412

99.125

0.391