Detailed information NTG0609655

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LCW13_RS00985
• Genome accession: NZ_CP084115
• Coordinates: 216435..217589 (+)
• Length: 384 a.a.
• NCBI ID: WP_043333447.1
• Uniprot ID: A0AAP4TZ60
• Organism: Cobetia amphilecti strain N-80
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 211435..222589

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LCW13_RS00965 (LCW13_00965)	-	212654..213235 (-)	582	WP_043333437.1	YggT family protein	-
LCW13_RS00970 (LCW13_00970)	proC	213385..214227 (-)	843	WP_043333440.1	pyrroline-5-carboxylate reductase	-
LCW13_RS00975 (LCW13_00975)	-	214316..215068 (-)	753	WP_043333442.1	YggS family pyridoxal phosphate-dependent enzyme	-
LCW13_RS00980 (LCW13_00980)	pilT	215264..216295 (+)	1032	WP_043333445.1	type IV pilus twitching motility protein PilT	Machinery gene
LCW13_RS00985 (LCW13_00985)	pilU	216435..217589 (+)	1155	WP_043333447.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LCW13_RS00990 (LCW13_00990)	-	217696..218844 (-)	1149	WP_043333449.1	anhydro-N-acetylmuramic acid kinase	-
LCW13_RS00995 (LCW13_00995)	tyrS	219000..220208 (+)	1209	WP_043333452.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 43054.35 Da
• Isoelectric Point: 6.3429

>NTDB_id=609655 LCW13_RS00985 WP_043333447.1 216435..217589(+) (pilU) [Cobetia amphilecti strain N-80]
MTPHEWLHELLQLMVSKGSSDLFISTGTPPQMKVNGRMVALGDKKLSVDQVRELVLAPMSDMQRERFEEEREANFAHSLP
GVGRFRISAFYQRSQMGMVIRRIQLSIPSLEELRLPEIIKGLSETKRGLVIFVGGTGAGKSTSLAAMIQHRNQTSSGHII
CIEDPIEYIHPHQRSIVTQREVGIDTESFEVALRNTLRQAPDVIMIGEIRSRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPEERHEQVWMDLSLNLKGIVAQQLLPHKSGNTTQRVPAIEVMLRSPLIVDLIRKGAVVEIKDVMKRSQQQGM
MTFDQSLYALHQQGLITEEVALAHADSANDLRLMIKFGDSDSAQEAQLDVLNAASRFSLQGDDD

Nucleotide

• Length: 1155 bp

>NTDB_id=609655 LCW13_RS00985 WP_043333447.1 216435..217589(+) (pilU) [Cobetia amphilecti strain N-80]
ATGACTCCTCATGAATGGCTGCATGAACTTCTGCAGTTGATGGTCAGCAAGGGCAGCTCGGACCTGTTCATCTCCACCGG
CACGCCGCCGCAGATGAAGGTCAATGGCCGCATGGTGGCGCTGGGTGACAAGAAGCTGAGTGTCGATCAGGTCAGAGAGC
TGGTGCTCGCGCCGATGAGTGACATGCAGCGCGAGCGCTTCGAGGAAGAGCGCGAGGCCAACTTCGCGCATAGCTTGCCG
GGGGTCGGGCGCTTCAGGATCAGCGCCTTCTACCAGCGTAGCCAGATGGGCATGGTGATTCGCCGCATCCAGCTGTCGAT
TCCCAGCCTGGAAGAGTTGCGGCTGCCGGAGATCATCAAGGGGCTTTCCGAGACCAAGCGTGGTCTGGTGATCTTCGTCG
GCGGCACCGGCGCCGGCAAGTCGACCTCGCTGGCGGCGATGATCCAGCACCGCAACCAGACATCGAGCGGGCATATCATC
TGCATCGAAGACCCGATCGAGTATATCCACCCTCACCAGCGCTCCATCGTGACGCAGCGTGAAGTGGGGATCGATACCGA
GTCCTTCGAGGTGGCGCTGCGCAATACGCTGCGTCAGGCGCCGGACGTGATAATGATCGGCGAGATCCGCTCGCGTGAGA
CCATGGAGCACGCGCTGACCTTCGCCGAGACCGGTCACCTGTGTCTTGCGACGTTGCATGCCAACAATGCCAACCAGGCG
CTGGACCGCATCATCCACTTCTTCCCGGAGGAGCGTCATGAACAGGTGTGGATGGATCTGTCGCTGAACCTCAAGGGCAT
CGTGGCCCAGCAGCTGTTGCCTCACAAGAGTGGCAACACCACCCAGCGCGTGCCGGCCATTGAGGTGATGCTGCGTTCGC
CGCTGATCGTCGACCTGATCCGCAAGGGCGCGGTGGTCGAGATCAAGGACGTCATGAAGCGCTCGCAGCAGCAGGGCATG
ATGACTTTCGATCAATCGCTGTACGCCTTGCATCAGCAGGGCTTGATCACCGAGGAAGTGGCATTGGCGCATGCCGATTC
CGCCAATGACCTGCGCCTGATGATCAAGTTCGGTGATTCCGACAGCGCCCAGGAAGCCCAGCTGGATGTCCTGAATGCCG
CCAGTCGCTTCTCGCTGCAAGGGGATGACGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (84-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	65.625	91.667	0.602
pilU	Vibrio cholerae strain A1552	56.64	96.094	0.544
pilU	Acinetobacter baylyi ADP1	55.932	92.188	0.516
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.348	89.844	0.398
pilT	Neisseria meningitidis 8013	40.816	89.323	0.365
pilT	Pseudomonas aeruginosa PAK	41.742	86.719	0.362