Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CYCME_RS00375 Genome accession   NC_021917
Coordinates   81022..82059 (-) Length   345 a.a.
NCBI ID   WP_020931789.1    Uniprot ID   S5TTN0
Organism   Cycloclasticus zancles 78-ME     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 76022..87059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYCME_RS00350 (CYCME_0069) - 77184..77708 (-) 525 WP_041932847.1 pilus assembly PilX family protein -
  CYCME_RS00355 (CYCME_0070) - 77711..78793 (-) 1083 WP_020931785.1 PilW family protein -
  CYCME_RS00360 (CYCME_0071) pilV 78790..79230 (-) 441 WP_020931786.1 type IV pilus modification protein PilV -
  CYCME_RS00365 (CYCME_0072) - 79221..79799 (-) 579 WP_020931787.1 GspH/FimT family pseudopilin -
  CYCME_RS00370 (CYCME_0073) pilU 79880..80992 (-) 1113 WP_020931788.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CYCME_RS00375 (CYCME_0074) pilT 81022..82059 (-) 1038 WP_020931789.1 type IV pilus twitching motility protein PilT Machinery gene
  CYCME_RS00380 (CYCME_0075) - 82184..82873 (+) 690 WP_020931790.1 YggS family pyridoxal phosphate-dependent enzyme -
  CYCME_RS00385 (CYCME_0076) proC 82907..83734 (+) 828 WP_020931791.1 pyrroline-5-carboxylate reductase -
  CYCME_RS00390 (CYCME_0077) - 83734..84288 (+) 555 WP_020931792.1 YggT family protein -
  CYCME_RS00395 (CYCME_0078) - 84516..86447 (+) 1932 WP_020931793.1 dynamin family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38703.38 Da        Isoelectric Point: 6.4341

>NTDB_id=60886 CYCME_RS00375 WP_020931789.1 81022..82059(-) (pilT) [Cycloclasticus zancles 78-ME]
MDISDLLTFSTKNNASDLHLSAGLPPMIRVNGDIRRINMPELEHKEVHSMIYDIMNDKQRRDYEEFLEVDFSFELPGTAR
FRVNAFNQDRGAAAVFRTIPVDILTLEDLNCPQSFKEIINVPRGLVLVTGPTGSGKSTTLAALMDYKNDNVYEHILTIED
PIEFVHKSKKCLINQREVHKHTHGFSEALRVALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPEGEKGMVRSMLSESLRAVIAQTLMKKIGGGRIAAWEIMTGTPAIRNLIREDKIAQMYSTIQTSRKDGMQTLDQHL
TELVQAGIVSRQDARMKAADKSTFN

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=60886 CYCME_RS00375 WP_020931789.1 81022..82059(-) (pilT) [Cycloclasticus zancles 78-ME]
ATGGATATTTCAGATTTACTAACCTTTAGCACAAAAAATAACGCATCGGACTTACATCTTTCAGCCGGCTTACCACCAAT
GATTCGTGTTAATGGTGATATTCGTCGAATCAATATGCCTGAACTAGAGCATAAAGAAGTTCACTCGATGATTTACGATA
TCATGAACGATAAACAGCGACGTGATTATGAAGAGTTCCTAGAAGTTGATTTTTCATTTGAATTGCCTGGTACGGCCCGT
TTTCGTGTTAATGCATTTAACCAGGATCGTGGAGCAGCTGCTGTATTTAGAACTATTCCCGTTGATATTCTTACCCTAGA
AGACTTAAATTGCCCGCAATCTTTTAAGGAGATCATCAATGTTCCACGCGGTCTTGTGCTAGTAACCGGCCCAACTGGGT
CAGGTAAATCCACCACGCTAGCAGCGTTGATGGATTATAAAAATGACAATGTATACGAACATATTCTCACTATCGAAGAT
CCAATTGAATTCGTTCATAAAAGCAAAAAGTGCCTTATTAATCAACGTGAAGTACACAAACATACCCATGGGTTCAGTGA
AGCCCTAAGAGTTGCGCTACGTGAAGACCCTGACATTATTCTTGTAGGTGAAATGCGAGACCTTGAGACCATTCGACTTG
CGTTAACCGCCGCAGAAACGGGGCACTTAGTTTTCGGTACACTACACACCAGCTCCGCCGCTAAAACCGTTGACCGGATT
ATTGATGTATTCCCAGAAGGCGAAAAGGGCATGGTGCGTTCCATGTTATCTGAATCACTTCGAGCTGTTATTGCCCAAAC
CTTGATGAAAAAAATTGGTGGTGGACGAATCGCCGCCTGGGAAATAATGACCGGCACCCCTGCCATACGTAATCTCATTA
GAGAGGATAAAATTGCACAAATGTACTCCACAATACAAACCAGTCGTAAAGATGGTATGCAAACACTTGATCAACACTTA
ACTGAACTCGTTCAAGCTGGGATTGTCTCGAGACAAGACGCCCGCATGAAAGCCGCTGATAAAAGCACGTTCAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S5TTN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.872

99.71

0.757
  pilT Acinetobacter nosocomialis M2

75

99.71

0.748
  pilT Acinetobacter baumannii D1279779

75

99.71

0.748
  pilT Acinetobacter baumannii strain A118

75

99.71

0.748
  pilT Pseudomonas stutzeri DSM 10701

74.709

99.71

0.745
  pilT Legionella pneumophila strain Lp02

72.965

99.71

0.728
  pilT Legionella pneumophila strain ERS1305867

72.965

99.71

0.728
  pilT Acinetobacter baylyi ADP1

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.681

0.667
  pilT Vibrio cholerae strain A1552

68.249

97.681

0.667
  pilT Neisseria meningitidis 8013

65.029

100

0.652
  pilT Neisseria gonorrhoeae MS11

64.74

100

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

100

0.487
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

39.766

99.13

0.394
  pilU Vibrio cholerae strain A1552

39.359

99.42

0.391

Multiple sequence alignment