Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CYCME_RS00370 Genome accession   NC_021917
Coordinates   79880..80992 (-) Length   370 a.a.
NCBI ID   WP_020931788.1    Uniprot ID   S5TC81
Organism   Cycloclasticus zancles 78-ME     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 74880..85992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYCME_RS00350 (CYCME_0069) - 77184..77708 (-) 525 WP_041932847.1 pilus assembly PilX family protein -
  CYCME_RS00355 (CYCME_0070) - 77711..78793 (-) 1083 WP_020931785.1 PilW family protein -
  CYCME_RS00360 (CYCME_0071) pilV 78790..79230 (-) 441 WP_020931786.1 type IV pilus modification protein PilV -
  CYCME_RS00365 (CYCME_0072) - 79221..79799 (-) 579 WP_020931787.1 GspH/FimT family pseudopilin -
  CYCME_RS00370 (CYCME_0073) pilU 79880..80992 (-) 1113 WP_020931788.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CYCME_RS00375 (CYCME_0074) pilT 81022..82059 (-) 1038 WP_020931789.1 type IV pilus twitching motility protein PilT Machinery gene
  CYCME_RS00380 (CYCME_0075) - 82184..82873 (+) 690 WP_020931790.1 YggS family pyridoxal phosphate-dependent enzyme -
  CYCME_RS00385 (CYCME_0076) proC 82907..83734 (+) 828 WP_020931791.1 pyrroline-5-carboxylate reductase -
  CYCME_RS00390 (CYCME_0077) - 83734..84288 (+) 555 WP_020931792.1 YggT family protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41267.22 Da        Isoelectric Point: 6.1484

>NTDB_id=60885 CYCME_RS00370 WP_020931788.1 79880..80992(-) (pilU) [Cycloclasticus zancles 78-ME]
MDFDALLQLVVHKKASDLFITTGWAPSLKINGELQAISKSRLSNSQTLEIVLSIMTPEQKEEFKTTNECQFAIQRHDIGR
FRVSAFTQRSSAGMVLRRIESKIPTAEELGIPPILNELAMTKRGLIIFVGATGTGKSTSLAAMVGHRNNNSTGHIITIED
PIEYLHSHAGCVVTQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRETMDHAIAFAETGHLCLSTLHANNANQALDRI
IHFFPEEAHNQLFMDLSLNLKAVIAQQLIPTIDGKGRKAVIEILINTPLASDYIRKGEIHKLKDLMKKSVEHGMQTFDQA
LYELYKKGEISYENALMYADSQNEVRLMIKLGSEASSDFNDESIHLIDTE

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=60885 CYCME_RS00370 WP_020931788.1 79880..80992(-) (pilU) [Cycloclasticus zancles 78-ME]
ATGGATTTTGATGCCCTATTACAACTCGTTGTTCATAAAAAAGCATCTGACTTGTTTATTACAACTGGCTGGGCGCCCTC
TTTGAAAATAAATGGCGAATTACAAGCCATTTCTAAAAGTCGATTAAGCAATTCTCAAACTCTTGAAATAGTCCTAAGCA
TAATGACCCCCGAACAAAAAGAAGAGTTTAAGACCACTAACGAATGTCAATTTGCGATTCAAAGACATGACATTGGTCGC
TTTCGTGTGAGCGCGTTCACCCAAAGAAGCAGTGCGGGCATGGTATTACGACGAATTGAAAGTAAAATACCAACAGCAGA
AGAACTGGGCATACCCCCTATATTAAATGAGCTAGCGATGACGAAACGCGGTCTCATTATTTTTGTTGGTGCAACAGGAA
CCGGCAAGTCCACATCATTAGCGGCCATGGTCGGCCACCGTAACAACAATAGCACCGGCCATATTATTACTATAGAAGAC
CCTATTGAATATTTACACTCTCATGCTGGCTGTGTCGTTACGCAACGGGAAGTAGGCATTGATACTGAATCCTATGAAGT
AGCTTTAAAAAACACCCTAAGACAAGCGCCTGATGTGATTCTAATTGGTGAAATTCGAACACGAGAAACAATGGATCATG
CTATCGCGTTTGCCGAAACGGGACATTTATGCTTATCAACCTTGCATGCAAACAATGCGAACCAAGCACTTGATCGCATT
ATTCATTTTTTCCCCGAAGAAGCACATAACCAATTATTCATGGATTTATCACTCAATCTCAAAGCTGTAATAGCTCAACA
ATTGATACCCACAATCGATGGCAAAGGCCGAAAAGCTGTTATTGAAATACTCATAAACACGCCTCTTGCCTCAGATTACA
TTAGAAAAGGCGAAATACATAAGCTAAAAGATTTAATGAAAAAATCAGTTGAACACGGAATGCAAACCTTTGATCAAGCA
TTATATGAATTGTATAAAAAAGGTGAGATTAGCTATGAAAATGCTCTGATGTATGCTGATTCACAAAATGAAGTAAGGCT
GATGATTAAACTTGGCTCTGAAGCTTCTTCAGACTTTAATGACGAATCAATCCATTTAATAGATACGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S5TC81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.715

96.757

0.665
  pilU Acinetobacter baylyi ADP1

65.47

97.838

0.641
  pilU Vibrio cholerae strain A1552

55.616

98.649

0.549
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.835

98.378

0.392
  pilT Legionella pneumophila strain Lp02

41.194

90.541

0.373
  pilT Legionella pneumophila strain ERS1305867

41.194

90.541

0.373
  pilT Pseudomonas aeruginosa PAK

40.597

90.541

0.368
  pilT Acinetobacter baylyi ADP1

39.296

92.162

0.362

Multiple sequence alignment