Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LCH18_RS13600 Genome accession   NZ_CP083947
Coordinates   2797044..2798081 (+) Length   345 a.a.
NCBI ID   WP_224467305.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain CSUSB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2792044..2803081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH18_RS13590 (LCH18_13590) - 2795308..2796042 (-) 735 WP_224467302.1 hypothetical protein -
  LCH18_RS13595 (LCH18_13595) - 2796232..2796921 (-) 690 WP_224467303.1 YggS family pyridoxal phosphate-dependent enzyme -
  LCH18_RS13600 (LCH18_13600) pilT 2797044..2798081 (+) 1038 WP_224467305.1 type IV pilus twitching motility protein PilT Machinery gene
  LCH18_RS13605 (LCH18_13605) pilU 2798108..2799229 (+) 1122 WP_224467307.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LCH18_RS13610 (LCH18_13610) fur 2799289..2799726 (-) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  LCH18_RS13615 (LCH18_13615) - 2799839..2800237 (+) 399 WP_087528300.1 outer membrane protein assembly factor BamE -
  LCH18_RS13620 (LCH18_13620) - 2800284..2800607 (-) 324 WP_224467308.1 RnfH family protein -
  LCH18_RS13625 (LCH18_13625) - 2800607..2801689 (-) 1083 WP_224467310.1 hypothetical protein -
  LCH18_RS13630 (LCH18_13630) - 2801832..2802287 (-) 456 WP_078389448.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38278.93 Da        Isoelectric Point: 6.6658

>NTDB_id=608764 LCH18_RS13600 WP_224467305.1 2797044..2798081(+) (pilT) [Acinetobacter johnsonii strain CSUSB1]
MDITELLAFSAKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDFEEKLETDFSFEVPSIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQTFKELCELPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMSTLDQSL
KNLVTKGVISPQTARTAAKQPETFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=608764 LCH18_RS13600 WP_224467305.1 2797044..2798081(+) (pilT) [Acinetobacter johnsonii strain CSUSB1]
ATGGATATCACAGAATTACTAGCCTTTTCGGCTAAGAACGGGGCATCAGATTTACACTTATCTGCAGGTATGCCACCGAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATCAATTTACCAGCGTTAGAACACAAAGAAGTGCATAAACTGGTCTATGACA
TCATGAATGACAAACAACGTCGTGATTTTGAAGAGAAATTAGAAACAGACTTTTCCTTTGAAGTTCCAAGTATTGCTCGT
TTTCGTGTGAACGCATTCAACCAAAACCGCGGTGCAGGTGCAGTATTCCGTACCATTCCGTCTAAAGTCCTGACCATGGA
AGACTTAGGTTTAGGACAAACCTTCAAAGAGTTATGTGAATTGCCACGTGGTTTAGTTTTAGTGACGGGGCCTACTGGTT
CTGGTAAATCTACCACCTTGGCGGCAATGATCGATTATATTAACGATAACCGCTATGACCATATCTTAACGGTTGAAGAC
CCAATCGAATTTGTACACCAGTCAAAAAAATGCTTGATCAACCAACGTGAAGTACACCGTGATACACATGGTTTTAACGA
AGCGCTGCGTTCTGCACTCCGTGAAGACCCCGATATTATCCTTGTGGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CACTCACTGCCGCTGAAACGGGTCACTTGGTCTTCGGTACACTACATACGACCTCTGCGGCAAAAACTATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTTCGAGCCATGTTATCTGAATCGCTACAAGCCGTGATTTCTCAAAC
CTTACTCAAGAAAAATGGCGGTGGCCGTGTTGCTGCGCATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAACAAAGTGGCTCAGATGTATTCTGCTATTCAGACTGGTGCAAACTACGGTATGTCCACATTGGATCAAAGTCTG
AAAAACTTGGTCACCAAAGGGGTGATCAGTCCGCAGACCGCGCGTACAGCTGCCAAGCAACCTGAAACCTTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.203

100

0.942
  pilT Acinetobacter baumannii D1279779

94.203

100

0.942
  pilT Acinetobacter baumannii strain A118

94.203

100

0.942
  pilT Acinetobacter baylyi ADP1

86.047

99.71

0.858
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

67.246

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.452

97.391

0.667
  pilT Vibrio cholerae strain A1552

68.452

97.391

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.525
  pilU Vibrio cholerae strain A1552

42.183

98.261

0.414
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Acinetobacter baylyi ADP1

39.942

99.42

0.397